呼吸道合胞病毒毛細支氣管炎患兒外周血T細胞亞群、IL-4、IFN-γ、IL-17的水平及意義
發(fā)布時間：2019-05-07 07:35
【摘要】：目的察探究外周血中Treg細胞、T細胞群炎性細胞因子IL-4 IFN-γIL-17在RSV感染的毛細支氣管炎患兒發(fā)病病程展中的意義方法選2015年7~2016年6期間臨沂人民院收治的毛細支氣管炎院患兒1038例作臨床研究對象,使用免疫熒法對患兒咽部分泌物行7種道病毒檢選RSV檢性的患兒88例,按照疾病展程度將患兒輕癥組45例和伴明顯喘息癥狀的癥組43例選時期因非感染疾病入院的患兒作對照組45例用流式細胞儀檢患兒外周血中Treg細胞T細胞群情況用酶聯(lián)免疫試驗ELISA抗夾心法檢患兒血清中IL-4 IFN-γIL-17等炎性細胞因子表達水平結果1 1038例毛細支氣管炎患兒中7種道病毒總檢率是42.29%,中RSV 65.83%2癥組和輕癥組RSV感染的毛細支氣管炎患兒外周血Treg細胞均明顯于常對照組兒童P0.05癥組RSV感染的毛細支氣管炎患兒外周血Treg細胞于輕癥組患兒P0.05,組間差明顯統(tǒng)計學意義3癥組和輕癥組RSV感染的毛細支氣管炎患兒常對照組兒童較,外周血中CD8~+T細胞均于常對照組兒童P0.05,CD4~+T細胞和CD4~+/CD8~+T細胞值均高于常對照組兒童P0.05癥組患兒外周血CD8~+T細胞于輕癥組患兒P0.05,CD4~+T細胞和CD4~+/CD8~+T細胞值高于輕癥組患兒P0.05,差統(tǒng)計學意義4癥組和輕癥組RSV感染的毛細支氣管炎患兒外周血IL-4 IL-17水平均明顯高于常對照組兒童P0.05,IFN-γ水平明顯于常對照組兒童P0.05癥組患兒外周血IL-4 IL-17水平高于輕癥組患兒P0.05,IFN-γ水平于輕癥組患兒P0.05,組間差統(tǒng)計學意義。結論1患兒外周血Treg細胞RSV感染的毛細支氣管炎發(fā)展呈負相關2患兒外周血CD8~+T細胞RSV感染的毛細支氣管炎發(fā)展呈負相關,外周血CD4~+T細胞和CD4~+/CD8~+T細胞值RSV感染發(fā)展呈相關3患兒外周血中IL-4 IL-17水平RSV感染展程度呈相關,IFN-γ水平疾病展呈負相關。
[Abstract]:Objective to investigate the Treg cells in peripheral blood. Significance of T-cell inflammatory cytokine IL-4 IFN- 緯-IL-17 in the pathogenesis of RSV-infected bronchiolitis methods 1038 children with bronchiolitis admitted to Linyi people's Hospital from July 2015 to June 2016 were selected as clinical subjects. Using immunofluorescence method, 88 children with RSV were tested for 7 kinds of viruses in pharyngeal secretion. According to the degree of disease spread, 45 cases of mild group and 43 cases of group with obvious wheezing symptoms were selected as control group, 45 cases of non-infectious diseases were selected as control group. Flow cytometry was used to detect the T-cell population of Treg cells in peripheral blood of children with mild disease and 43 cases with obvious wheezing symptoms. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-4 IFN- 緯-IL-17 and other inflammatory cytokines in serum of children with bronchiolitis. The total detection rate of 7 kinds of viruses in 1 1038 children with bronchiolitis was 42.29%. Treg cells in peripheral blood of patients with bronchiolitis infected with RSV in RSV 65.83% 2 group and mild group were significantly higher than those in children with bronchiolitis infected with RSV in the normal control group (P0.05) and in the mild group (P0.05). The difference between the three groups was statistically significant. The CD8~ T cells in the peripheral blood of children with bronchiolitis infected by RSV in the three groups were significantly higher than those in the control group (P0.05, P 0.05, P < 0.05). The values of CD4~ T cells and CD4~ / CD8~ T cells were higher than those of the normal control group in the peripheral blood CD8~ T cells, CD4 ~ T cells and CD4~ / CD8~ T cells in the mild group were higher than those in the mild group (P 0.05, P < 0.05, P < 0.01, P < 0.01). The IL-4 IL-17 level in peripheral blood of bronchiolitis patients with RSV infection was significantly higher than that in normal controls (P 0.05, P < 0.01), but there was no significant difference between the two groups (P < 0.05). The level of IFN- 緯 in peripheral blood was significantly higher in P05 group than that in mild group (P0.05), and the level of IFN-緯 in mild group was significantly higher than that in mild group (P0.05), and the difference between the two groups was statistically significant. Conclusion (1) the development of bronchiolitis infected with RSV of peripheral blood Treg cells is negatively correlated with the development of bronchiolitis infected with RSV of peripheral blood CD8~ T cells. The peripheral blood CD4~ T cells and CD4~ / CD8~ T cell values were correlated with the development of RSV infection. 3. The degree of RSV infection in peripheral blood was correlated with the degree of RSV infection in the peripheral blood, and there was a negative correlation between the disease spread of IFN- 緯 level and the peripheral blood IL-4 IL-17 T cells.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R725.6
本文編號：2470882
[Abstract]:Objective to investigate the Treg cells in peripheral blood. Significance of T-cell inflammatory cytokine IL-4 IFN- 緯-IL-17 in the pathogenesis of RSV-infected bronchiolitis methods 1038 children with bronchiolitis admitted to Linyi people's Hospital from July 2015 to June 2016 were selected as clinical subjects. Using immunofluorescence method, 88 children with RSV were tested for 7 kinds of viruses in pharyngeal secretion. According to the degree of disease spread, 45 cases of mild group and 43 cases of group with obvious wheezing symptoms were selected as control group, 45 cases of non-infectious diseases were selected as control group. Flow cytometry was used to detect the T-cell population of Treg cells in peripheral blood of children with mild disease and 43 cases with obvious wheezing symptoms. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-4 IFN- 緯-IL-17 and other inflammatory cytokines in serum of children with bronchiolitis. The total detection rate of 7 kinds of viruses in 1 1038 children with bronchiolitis was 42.29%. Treg cells in peripheral blood of patients with bronchiolitis infected with RSV in RSV 65.83% 2 group and mild group were significantly higher than those in children with bronchiolitis infected with RSV in the normal control group (P0.05) and in the mild group (P0.05). The difference between the three groups was statistically significant. The CD8~ T cells in the peripheral blood of children with bronchiolitis infected by RSV in the three groups were significantly higher than those in the control group (P0.05, P 0.05, P < 0.05). The values of CD4~ T cells and CD4~ / CD8~ T cells were higher than those of the normal control group in the peripheral blood CD8~ T cells, CD4 ~ T cells and CD4~ / CD8~ T cells in the mild group were higher than those in the mild group (P 0.05, P < 0.05, P < 0.01, P < 0.01). The IL-4 IL-17 level in peripheral blood of bronchiolitis patients with RSV infection was significantly higher than that in normal controls (P 0.05, P < 0.01), but there was no significant difference between the two groups (P < 0.05). The level of IFN- 緯 in peripheral blood was significantly higher in P05 group than that in mild group (P0.05), and the level of IFN-緯 in mild group was significantly higher than that in mild group (P0.05), and the difference between the two groups was statistically significant. Conclusion (1) the development of bronchiolitis infected with RSV of peripheral blood Treg cells is negatively correlated with the development of bronchiolitis infected with RSV of peripheral blood CD8~ T cells. The peripheral blood CD4~ T cells and CD4~ / CD8~ T cell values were correlated with the development of RSV infection. 3. The degree of RSV infection in peripheral blood was correlated with the degree of RSV infection in the peripheral blood, and there was a negative correlation between the disease spread of IFN- 緯 level and the peripheral blood IL-4 IL-17 T cells.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R725.6
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