【摘要】：目的研究急性期毛細支氣管炎的免疫病理狀態(tài)。 方法選擇46例急性期毛細支氣管炎患兒(其中輕癥組24例,重癥組22例)、20例普通病毒性肺炎患兒和15例健康嬰兒為研究對象,酶聯(lián)免疫吸附法測定血清IL-17、IFN-γ水平,化學(xué)發(fā)光法測定血清IgE水平。 結(jié)果(1)各組間患兒血清IL-17水平的比較：急性期毛細支氣管炎患兒血清IL-17水平為(26.91±7.13)ng/L,較普通病毒性肺炎組[(21.96±5.62)ng/L]和對照組[(13.56±5.18)ng/L]均顯著升高,差異有顯著意義(q=4.04、9.81,P0.01)；普通病毒性肺炎組患兒血清IL-17水平較對照組也顯著升高,差異有顯著意義(q=5.37,P0.01)；急性期毛細支氣管炎輕癥組患兒血清IL-17水平[(26.54±7.93)ng/L]與重癥組[(27.32±6.31)ng/L]比較差異無顯著意義(t=0.37,P=0.71)。 (2)各組間患兒血清IFN-γ水平的比較：急性期毛細支氣管炎患兒血清IFN-γ水平為(7.07±1.86)ng/L,較普通病毒性肺炎組[(9.12±1.89)ng/L]和對照組[(8.95±1.64)ng/L]顯著降低,差異有極顯著意義(q=5.91、4.89,P0.01)；而普通病毒性肺炎組血清IFN-γ水平較對照組差異無顯著意義(q=0.38,P0.05)。急性期毛細支氣管炎重癥組患兒血清IFN-γ水平[(6.13±1.56)ng/L]較輕癥組[(7.93±1.72)ng/L]明顯降低,差異有顯著意義(t=3.7,P0.01)。 (3)各組間患兒血清IgE水平的比較：急性期毛細支氣管炎患兒血清IgE水平[(171.77±58.19)IU/ml]較普通病毒性肺炎組[(51.38±12.44)IU/ml]及對照組[(43.95±12.81)IU/ml]均顯著升高,差異有顯著意義(q=18.08、18.65,P0.01)；急性期毛細支氣管炎重癥組患兒血清1gE水平[(189.27±48.49)IU/m]]較輕癥組[(155.73±62.56)IU/ml]明顯減低,差異有顯著意義(t=2.02,P0.05)。 結(jié)論毛細支氣管炎患兒急性期Thl細胞活性受抑、Th17細胞功能顯著增強,在其發(fā)病機制中發(fā)揮重要作用。
[Abstract]:Objective to study the immunopathologic status of acute bronchiolitis. Methods 46 children with acute bronchiolitis (24 cases in mild group and 22 cases in severe group), 20 children with common viral pneumonia and 15 healthy infants were selected as subjects. Serum IL-17, was measured by enzyme linked immunosorbent assay (Elisa). The level of IFN- 緯 was measured by chemiluminescence method. The serum IgE level was determined by chemiluminescence. Results (1) the serum IL-17 levels in children with acute bronchiolitis were (26.91 鹵7.13) ng/L, and (26.91 鹵7.13) ng/L, respectively. Compared with the common viral pneumonia group [(21.96 鹵5.62) ng/L] and the control group [(13.56 鹵5.18) ng/L], the difference was significant (Q = 4.04, 9.81, P0.01). The level of serum IL-17 in the common viral pneumonia group was significantly higher than that in the control group (Q = 5.37, P0.01). There was no significant difference in serum IL-17 level between acute bronchitis group [(26.54 鹵7.93) ng/L] and severe group [(27.32 鹵6.31) ng/L] (t = 0.37, P = 0.71). (2) comparison of serum IFN- 緯 levels in children with acute bronchiolitis: the serum IFN- 緯 levels in children with acute bronchiolitis were (7.07 鹵1.86) ng/L,. Compared with the normal viral pneumonia group [(9.12 鹵1.89) ng/L] and the control group [(8.95 鹵1.64) ng/L], the difference was very significant (Q = 5.91, 4.89, P0.01). There was no significant difference in the level of serum IFN- 緯 between the common viral pneumonia group and the control group (Q = 0.38, P0.05). The serum IFN- 緯 level in acute bronchitis group [(6.13 鹵1.56) ng/L] was significantly lower than that in mild group [(7.93 鹵1.72) ng/L], the difference was significant (t = 3.7, P0.01). (3) comparison of serum IgE levels in children with acute bronchiolitis [(171.77 鹵58.19) IU/ml] compared with common viral pneumonia group [(51.38 鹵12.44) IU/ml] and the control group [(51.38 鹵12.44) IU/ml]. [(43.95 鹵12.81) IU/ml] were significantly increased. The difference was significant (Q = 18.08, 18.65, P0.01); The serum 1gE level in acute bronchiolitis group [(189.27 鹵48.49) IU/m] was significantly lower than that in mild group [(155.73 鹵62.56) IU/ml], the difference was significant (t = 2.02, P0.05). Conclusion in the acute stage of bronchiolitis, the activity of Thl cells is inhibited and the function of Th17 cells is significantly enhanced, which plays an important role in the pathogenesis of bronchiolitis.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R725.6

【參考文獻】

相關(guān)期刊論文 前5條

1 吳學(xué)忠;;Th17細胞的研究進展[J];國際免疫學(xué)雜志;2007年06期

2 余嫻;程翔;廖玉華;丁英俊;付卿卿;謝江嬌;姚瑞;陳勇;唐婷婷;;急性冠狀動脈綜合征患者Th17細胞檢測及意義[J];臨床心血管病雜志;2008年02期

3 陳旭娥;劉志香;吳艷;劉厚君;李家文;;尋常型銀屑病皮損中Th17細胞相關(guān)因子的表達[J];中國麻風(fēng)皮膚病雜志;2008年03期

4 全國兒童哮喘防治協(xié)作組;中國城區(qū)兒童哮喘患病率調(diào)查[J];中華兒科雜志;2003年02期

5 羅家明;秦新月;陳黎燕;尹紅蕾;;甲基強的松龍抑制實驗性自身免疫性腦脊髓炎IL-23/IL-17軸機制研究[J];中國神經(jīng)精神疾病雜志;2008年06期

，

本文編號：2442863