【摘要】：目的檢測金屬蛋白酶家族中金屬蛋白酶7(MMP-7)和金屬蛋白酶19(MMP-19)在幼鼠膽道閉鎖動物模型中的表達(dá)情況,探討其在膽道閉鎖肝纖維化中的作用及機(jī)制。方法選取出生3 d的Wistar幼鼠132只作為實(shí)驗(yàn)組,采用肝外膽管結(jié)扎的方法制作膽道閉鎖動物模型,62只幼鼠作為對照組,僅行開腹探查術(shù)。于術(shù)后3 d、7 d、14 d、21 d、28 d取肝臟組織,應(yīng)用免疫組化、Western blotting及實(shí)時PCR等方法分別檢測MMP-7及MMP-19在2組肝臟組織中的分布和表達(dá)情況。結(jié)果實(shí)驗(yàn)組幼鼠在術(shù)后24h即表現(xiàn)出梗阻性黃疸的臨床癥狀;HE染色及Masson染色均發(fā)現(xiàn)肝臟組織纖維化逐漸加重;免疫組化、Western blotting、實(shí)時PCR等均發(fā)現(xiàn)實(shí)驗(yàn)組MMP-7的表達(dá)明顯高于對照組,且逐漸升高;MMP-19在各時間段表達(dá)逐漸增強(qiáng),但均低于對照組。結(jié)論 MMP-7和MMP-19在膽道閉鎖肝纖維化中可能發(fā)揮重要作用。
[Abstract]:Objective to investigate the expression of metalloproteinase 7 (MMP-7) and metalloproteinase 19 (MMP-19) in the biliary atresia model of young rats and to explore the role and mechanism of metalloproteinase 7 (MMP-7) and metalloproteinase 19 (MMP-19) in hepatic fibrosis of biliary atresia. Methods 132 young Wistar rats were selected as experimental group, and 62 young rats were used as control group to make biliary atresia model by ligation of extrahepatic bile duct. Liver tissues were taken at 3 days, 7 days, 14 days, 21 days and 28 days after operation. The distribution and expression of MMP-7 and MMP-19 in the liver tissues of the two groups were detected by immunohistochemical, Western blotting and real-time PCR methods. Results the young rats in the experimental group showed the clinical symptoms of obstructive jaundice 24 hours after operation, and liver fibrosis was gradually aggravated by HE staining and Masson staining. The expression of MMP-7 in the experimental group was significantly higher than that in the control group, and the expression of MMP-19 was gradually increased, but lower than that in the control group. Conclusion MMP-7 and MMP-19 may play an important role in biliary atresia liver fibrosis.
【作者單位】： 中國醫(yī)科大學(xué)附屬盛京醫(yī)院小兒外科;
【基金】：國家自然科學(xué)基金(81270437)
【分類號】：R-332;R725.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 葛軍濤;李龍;魏延棟;王海斌;喬國梁;張震;劉W，

本文編號：2416206