【摘要】：目的探討原發(fā)性腎病綜合征(PNS)患兒腎組織中瞬時(shí)受體電位陽(yáng)離子通道蛋白6(TRPC6)的表達(dá)與足細(xì)胞損傷的關(guān)系及其臨床意義。方法獲取18例PNS患兒的腎組織,常規(guī)切片染色光鏡觀察腎臟組織病理改變,電鏡觀察足細(xì)胞的結(jié)構(gòu)變化,分別用q PCR和免疫組化測(cè)定組織中TRPC6 m RNA和蛋白的表達(dá),并將TRPC6 m RNA表達(dá)分別與血清白蛋白(Alb)、肌酐(Cr)、三酰甘油(TG)、膽固醇(Tch)、補(bǔ)體C3水平及24 h尿蛋白定量和評(píng)估的腎小球?yàn)V過(guò)率(e GFR)進(jìn)行相關(guān)性分析。結(jié)果 PNS患兒腎組織中TRPC6蛋白表達(dá)較對(duì)照組上調(diào),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。PNS患兒腎組織中TRPC6 m RNA的相對(duì)表達(dá)量與腎組織TRPC6蛋白表達(dá)呈顯著正相關(guān)(r=0.508,P0.05),與血清Alb、Cr、TG、Tch、補(bǔ)體C3、e GFR水平以及24 h尿蛋白定量無(wú)相關(guān)性(P0.05)。結(jié)論 PNS患兒的病理類型仍以足細(xì)胞病變?yōu)橹?其腎小球足細(xì)胞中TRPC6蛋白表達(dá)升高。TRPC6檢測(cè)可能有助于足細(xì)胞病變?cè)\斷。
[Abstract]:Objective to investigate the relationship between the expression of transient receptor potential cationic channel protein 6 (TRPC6) and podocyte injury in children with primary nephrotic syndrome (PNS) and its clinical significance. Methods the renal tissues of 18 children with PNS were obtained. The pathological changes of renal tissues were observed by light microscopy, and the structural changes of podocytes were observed by electron microscope. The expression of TRPC6 m RNA and protein in the tissues were detected by Q PCR and immunohistochemistry, respectively. The expression of TRPC6 m RNA was correlated with serum albumin (Alb), creatinine (Cr), triacylglycerol (TG), cholesterol (Tch), complement C3 and 24 h urinary protein quantification and glomerular filtration rate (e GFR). Results the expression of TRPC6 protein in renal tissue of PNS children was significantly higher than that of control group (P0.05). The relative expression of TRPC6 m RNA was positively correlated with the expression of TRPC6 protein in renal tissue of). PNS children (r = 0.508 P 0.05). There was no correlation with the level of serum Alb,Cr,TG,Tch, complement C _ 3e GFR and 24 h urine protein (P0.05). Conclusion the pathological types of PNS are still mainly podocytes, and the expression of TRPC6 protein in glomerular podocytes is increased. TRPC6 detection may be helpful in the diagnosis of podocytes.
【作者單位】： 上海市兒童醫(yī)院上海交通大學(xué)附屬兒童醫(yī)院腎臟風(fēng)濕科;
【基金】：上海市衛(wèi)生局級(jí)青年課題(No.2009Y048);上海市衛(wèi)生局級(jí)課題(No.2009094) 上海市兒童醫(yī)院優(yōu)秀青年培養(yǎng)計(jì)劃(2013)
【分類號(hào)】：R726.9

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