發(fā)布時間：2018-12-12 01:49

【摘要】：[目的]分析支氣管哮喘患兒與正常兒童呼吸道菌群的差異。[方法]6-14歲兒童為研究對象,分為三組,每組20人:A、哮喘急性發(fā)作期組(A組);B、上述患兒經(jīng)對癥治療后一周癥狀緩解為癥狀緩解期組(B組);C、對照組是正常兒童組(C組)。被檢兒童均取咽拭子,提取樣本細(xì)菌總DNA,進(jìn)一步對細(xì)菌16S rDNA V3可變區(qū)進(jìn)行PCR擴(kuò)增和DGGE電泳,獲得呼吸道中細(xì)菌分子指紋圖譜,利用Quantity One軟件分別對呼吸道菌群進(jìn)行多樣性分析,利用SPSS21.0軟件進(jìn)行統(tǒng)計學(xué)分析。[結(jié)果]各組呼吸道菌群比較:1、A組呼吸道菌群的條帶數(shù)S'、多樣性指數(shù)H'、和優(yōu)勢度指數(shù)D'顯著高于C組(P0.05)。2、A組與B組呼吸道菌群相比較,細(xì)菌的條帶數(shù)S'、多樣性指數(shù)H'均有統(tǒng)計學(xué)意義,均勻度指數(shù)E、優(yōu)勢度指數(shù)D'無統(tǒng)計學(xué)意義。3、B組與C組相比在條帶數(shù)S'、多樣性指數(shù)H'、均勻度指數(shù)E和豐富度指數(shù)D'均無明顯差異。[結(jié)論]1、哮喘急性發(fā)作期組兒童菌群總體數(shù)量更豐富,種類更多,優(yōu)勢菌群更為突出。2、哮喘急性發(fā)作期組與癥狀緩解期組患兒相比,其呼吸道菌群條帶數(shù)及多樣性指數(shù)均較高,而均勻度指數(shù)及優(yōu)勢度無明顯差異。3、哮喘癥狀緩解期兒童呼吸道菌群與正常兒童相比多樣性分析均無統(tǒng)計學(xué)差異。
[Abstract]:[objective] to analyze the difference of respiratory tract flora between children with bronchial asthma and normal children. [methods] Children aged 6-14 years were divided into three groups with 20 persons in each group: group A, group A (group A, group); B,), the symptoms were relieved to remission group (group B) after one week of symptomatic treatment; C, the control group was normal children group (group C). The throat swabs were taken from all the children tested, and the total DNA, was extracted from the sample. The 16s rDNA V3 variable region was amplified by PCR and DGGE electrophoresis, and the molecular fingerprint of the bacteria in the respiratory tract was obtained. The diversity of respiratory tract flora was analyzed by Quantity One software and statistical analysis by SPSS21.0 software. [results] Respiratory tract flora of group A was significantly higher than that of group C (P 0.05). 2Respiratory bacterial flora of group A was higher than that of group B (P 0.05), and the number of bands, diversity index (H) and dominance index (D') of group A were significantly higher than those of group C (P 0.05). The number of bands and the diversity index (H') of bacteria were statistically significant, but the evenness index (E) and dominance index (D') were not statistically significant. There was no significant difference in evenness index E and richness index D'. [conclusion] 1. The total number, species and dominant flora of children in acute asthma attack group were more abundant, more and more prominent. 2. Compared with symptom remission group, the children in acute asthma attack group were more abundant than those in symptom remission group. The number of bacterial bands and diversity index of respiratory tract were higher, but there was no significant difference in evenness index and dominance. 3. There was no significant difference in diversity analysis of respiratory tract bacteria between children with asthma symptom remission and normal children.
【學(xué)位授予單位】：東南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R725.6

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