【摘要】：目的:通過研究川崎病(Kawasaki disease,KD)患兒各期血漿中人軟骨糖蛋白39(Human Cartilage glycoprotein 39,HC gp-39)的表達水平及其與冠狀動脈損傷的關(guān)系,探討HC gp-39對KD診斷及病情評估的臨床意義。方法:于2015年3月至2016年2月選取在山西省兒童醫(yī)院心臟與風(fēng)濕免疫科確診為KD的患兒50例作為KD組,每例根據(jù)疾病的演變過程分為急性期和亞急性期,同時將KD組的急性期根據(jù)彩色多普勒超聲心動圖結(jié)果分為冠狀動脈損傷組(CAL)17例和無冠狀動脈損傷組(NCAL)33例,將同時段來其門診體檢的健康兒童20例作為健康對照組。采用雙抗體夾心固相酶聯(lián)免疫吸附法檢測所有究對象血漿中HC gp-39的表達水平,通過各組間的比較及相關(guān)性分析來探究川崎病患兒血漿中HC gp-39的表達水平及其與冠狀動脈損傷的關(guān)系。結(jié)果:定量檢測表明KD組各期血漿HC gp-39的表達水平均高于健康對照(F=94.42P0.05);KD組的急性期血漿HC gp-39的表達水平高于亞急性期(P0.05);KD組在急性期CAL組血漿HC gp-39的表達水平明顯高于NCAL組(t=4.39 P0.05);相關(guān)性分析顯示,患兒血漿中HC gp-39與血沉(r=0.78,P0.05)、血小板(r=0.80,P0.05)、C反應(yīng)蛋白(r=0.71,P0.05),均呈正相關(guān)。結(jié)論:人軟骨糖蛋白39在川崎病的發(fā)生發(fā)展中可能起到一定的作用,動態(tài)檢測血漿中HC gp-39可能對KD的早期診斷及病情評估有重要的臨床意義。
[Abstract]:Objective: to investigate the expression of human chondroglycoprotein 39 (Human Cartilage glycoprotein 39 HC gp-39 in plasma of children with Kawasaki disease (Kawasaki disease,KD) and its relationship with coronary artery injury. To explore the clinical significance of HC gp-39 in the diagnosis and evaluation of KD. Methods: from March 2015 to February 2016, 50 children with KD diagnosed in the Department of Cardiac and Rheumatological Immunology, Shanxi Children's Hospital, were selected as KD group. Each patient was divided into acute stage and subacute stage according to the evolution of the disease. At the same time, according to the results of color Doppler echocardiography, KD group was divided into coronary artery injury group (n = 17) and coronary artery injury group (n = 33). 20 healthy children who were examined at the same time were used as healthy control group. The expression of HC gp-39 in plasma was detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Elisa). To explore the expression level of HC gp-39 and its relationship with coronary artery injury in children with Kawasaki disease (Kawasaki disease). Results: quantitative analysis showed that the level of plasma HC gp-39 expression in KD group was higher than that in healthy control group (F=94.42P0.05); KD group was higher than that in subacute phase (P0.05). The expression of plasma HC gp-39 in KD group was significantly higher than that in NCAL group in acute phase CAL group (tr 4.39 P0.05). Correlation analysis showed that there was a positive correlation between plasma HC gp-39 and erythrocyte sedimentation rate (r = 0.78P), platelet (r = 0.80), C reactive protein (P 0.05). Conclusion: human cartilage glycoprotein 39 may play a role in the occurrence and development of Kawasaki disease. Dynamic detection of HC gp-39 in plasma may play an important role in the early diagnosis and evaluation of KD.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R725.4

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