【摘要】：目的目前仍不了解非酒精性脂肪性肝病(NAFLD)患兒人尾肢同源蛋白(Pygo2)血清水平在疾病進(jìn)展過程中作用,以及可否評(píng)估肝纖維化程度。文中旨在檢測(cè)肥胖兒童外周血中的Pygo2水平,并分析其與傳統(tǒng)肝纖維化血清學(xué)指標(biāo)之間的關(guān)系,從而評(píng)估NAFLD患兒Pygo2檢測(cè)的實(shí)際臨床價(jià)值。方法選擇2014年9月至2016年2月在安徽醫(yī)科大學(xué)附屬省立醫(yī)院和南京醫(yī)科大學(xué)第一附屬醫(yī)院就診的住院和門診肥胖患兒120例。參考NAFLD診療指南將120例肥胖患兒分為單純性肥胖組(n=44例,肝B超無彌漫性脂肪肝的肥胖兒童)、單純性非酒精性脂肪肝(NAFL)組(n=35例,肝B超提示有彌漫性脂肪肝但肝功能正常)、非酒精性脂肪肝炎(NASH)組(n=41例,肝B超提示有彌漫性脂肪肝且肝功能異常)。另選取18例健康志愿者為對(duì)照組。對(duì)照組及所有患者均收集外周血清,并分別采用酶聯(lián)免疫吸附方法測(cè)定血清Pygo2水平,放射免疫分析法檢測(cè)透明質(zhì)酸(HA)、Ⅲ型前膠原(PCⅢ)、Ⅳ型膠原(CⅣ)和層粘連蛋白(LN)和全自動(dòng)酶法測(cè)定血清丙氨酸氨基轉(zhuǎn)移酶(ALT)和γ-谷氨酰轉(zhuǎn)肽酶(γ-GT)表達(dá)量。結(jié)果 NAFL組、NASH組Pygo2表達(dá)量[52.1(12.3)、78.3(50.0)μg/L]較單純性肥胖組[43.2(18.7)μg/L]明顯升高(P0.05);NASH組Pygo2表達(dá)量較對(duì)照組[41.7(16.8)μg/L]、NAFL組明顯升高(P0.001)。NAFH組HA、CⅣ、LN、ALT、γ-GT表達(dá)量較對(duì)照組、單純性肥胖組、NAFL組明顯升高(P0.001)。血清Pygo2與HA、PCⅢ、CⅣ、ALT、γ-GT、LN呈顯著正相關(guān)(r=0.708、0.356、0.589、0.454、0.674,0.169,P0.05)。結(jié)論隨著Pygo2表達(dá)量水平升高,肝纖維化程度加重。Pygo2與HA、CⅣ、γ-GT等指標(biāo)顯著相關(guān),表明Pygo2亦可作為檢測(cè)NAFLD患兒肝纖維化的臨床評(píng)估指標(biāo)。
[Abstract]:Objective to investigate the role of human tail limb homologue protein (Pygo2) in the progression of (NAFLD) in children with non-alcoholic fatty liver disease and to evaluate the degree of hepatic fibrosis. The purpose of this study was to detect the level of Pygo2 in the peripheral blood of obese children and to analyze the relationship between the level of Pygo2 and the traditional serum markers of hepatic fibrosis, so as to evaluate the practical clinical value of Pygo2 detection in children with NAFLD. Methods from September 2014 to February 2016, 120 hospitalized and outpatient obese children were selected from Provincial Hospital affiliated to Anhui Medical University and the first affiliated Hospital of Nanjing Medical University. According to the NAFLD guidelines, 120 obese children were divided into simple obesity group (n = 44), obese children without diffuse fatty liver by liver ultrasound (n = 44) and simple non-alcoholic fatty liver (NAFL) group (n = 35). Liver B ultrasound showed diffuse fatty liver but normal liver function, and (NASH) group (n = 41) showed diffuse fatty liver with abnormal liver function. Another 18 healthy volunteers were selected as control group. Peripheral blood samples were collected from the control group and all the patients. Serum Pygo2 levels were measured by enzyme-linked immunosorbent assay (Elisa), and (HA), 鈪，

本文編號(hào)：2323415