【摘要】：【目的】 建立新生鼠腦缺氧模型,模擬臨床新生兒缺氧缺血性腦�。℉IE），通過對模型鼠電針刺激處理后，研究針刺對缺氧新生鼠腦中神經(jīng)源性分化因子(NeuroD)表達(dá)及細(xì)胞增殖的影響，為針刺治療HIE疾病提供實(shí)驗(yàn)依據(jù)。 【方法】 1.按照Grojean的方法：將出生10-24h內(nèi)SD新生大鼠置于100%氮?dú)獾臒o氧環(huán)境中，建立HIE模型，并對所做的動物模型進(jìn)行評價(jià)。 2.將動物隨機(jī)分為三組：對照組、缺氧組、缺氧+電針刺組，電針組采用臨床所用電子治療儀器，，選取大鼠相應(yīng)的穴位，每天持續(xù)30min，連續(xù)針刺10d后，通過RT-PCR、western blot及免疫熒光法檢測NeuroD，免疫熒光法檢測5-溴脫氧尿嘧啶核苷（BrdU）表達(dá)的變化。 【結(jié)果】 1.缺氧過程中新生大鼠均出現(xiàn)神經(jīng)異常的表現(xiàn)，如：躁動、意識障礙，并伴有全身紫紺，大腦皮質(zhì)HE染色可見缺氧組鼠腦神經(jīng)細(xì)胞損傷。 2.免疫熒光示：神經(jīng)干細(xì)胞聚集區(qū)大腦皮質(zhì)、海馬、SVZ等部位有NeuroD的表達(dá)。RT-PCR結(jié)果示：針刺組NeuroD mRNA表達(dá)量高于均正常組和缺氧組（P＜0.01）。Western blot結(jié)果示：針刺組NeuroD/β-actin平均光密度均高于正常組和缺氧組（P＜0.01）。針刺組NeuroD蛋白平均熒光強(qiáng)度均高于正常組和缺氧組（P＜0.01）；針刺組SVZ、DG部位BrdU表達(dá)量均高于缺氧組和對照組（P＜0.01）。 【結(jié)論】 電針刺激能使鼠腦神經(jīng)干細(xì)胞聚集區(qū)的大腦皮質(zhì)、海馬、SVZ等部NeuroD表達(dá)量增高，并可誘導(dǎo)細(xì)胞增殖，可能參與受損神經(jīng)組織修復(fù)和功能重建。
[Abstract]:[objective] to establish a neonatal model of hypoxic brain, and to simulate the clinical hypoxic-ischemic encephalopathy (HIE),) of neonatal hypoxic-ischemic encephalopathy (HIE). To study the effect of acupuncture on the expression of neurogenic differentiation factor (NeuroD) and cell proliferation in the brain of hypoxic neonatal rats, and to provide experimental evidence for acupuncture treatment of HIE disease. [methods] 1. According to the method of Grojean, the neonatal rats of SD were placed in 100% nitrogen anaerobic environment within 10-24 hours. The HIE model was established, and the animal model was evaluated. 2. The animals were randomly divided into three groups: control group, anoxic electroacupuncture group, electroacupuncture group and electroacupuncture group. The rats were selected corresponding acupoints for 30 minutes every day, after 10 days of continuous acupuncture, RT-PCR, was adopted. The expression of 5-bromodeoxyuridine (5-bromodeoxyuridine) (BrdU) was detected by western blot and NeuroD, immunofluorescence assay. [result] 1. During anoxia, the neonatal rats showed abnormal neurological manifestations, such as restlessness, disturbance of consciousness, and cyanosis. HE staining of cerebral cortex showed brain nerve cell damage in hypoxic group. 2. Immunofluorescence: neural stem cell accumulation, cerebral cortex, hippocampus, The expression of NeuroD was found in SVZ and other parts. RT-PCR results showed that the expression of NeuroD mRNA in acupuncture group was higher than that in normal group and hypoxia group (P < 0. 01). Western blot): the average optical density of NeuroD/ 尾-actin in acupuncture group was higher than that in normal group and deficient group (P < 0. 01). Oxygen group (P < 0.01). The average fluorescence intensity of NeuroD protein in acupuncture group was higher than that in normal group and hypoxia group (P < 0. 01), and the expression of BrdU in SVZ,DG in acupuncture group was higher than that in hypoxia group and control group (P < 0. 01). [conclusion] electroacupuncture can increase the expression of NeuroD in cerebral cortex, hippocampus and SVZ, and induce cell proliferation, which may be involved in the repair and functional reconstruction of injured nerve tissue.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R246.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 莊明華;駱健明;劉明發(fā);白曄;;針刺任脈、督脈及膀胱經(jīng)促進(jìn)新生兒缺血缺氧性腦病鼠模型腦內(nèi)神經(jīng)干細(xì)胞增殖[J];中華神經(jīng)醫(yī)學(xué)雜志;2006年07期

2 劉曉艷;徐劍文;王瑋;;短暫性缺氧對鼠腦神經(jīng)源性分化因子表達(dá)的影響[J];解剖學(xué)研究;2008年06期

3 袁清順;七星針為主治療小兒腦性癱瘓260例療效觀察[J];中國針灸;1994年01期

4 李林;魏海峰;張?zhí)m;楚晉;趙玲;;中醫(yī)“腎生髓,腦為髓�！爆F(xiàn)代生物學(xué)基礎(chǔ)探討[J];中國中藥雜志;2006年17期

5 趙文樹;趙文麟;姜超;孫申田;;從內(nèi)源神經(jīng)干細(xì)胞神經(jīng)再生探討針灸治療缺血性腦中風(fēng)的機(jī)理[J];針灸臨床雜志;2006年07期

6 楊巍;王曉明;;微電流脈沖體外誘導(dǎo)神經(jīng)干細(xì)胞增殖分化的研究[J];中國實(shí)驗(yàn)診斷學(xué);2010年05期

本文編號：2306001