【摘要】：目的:觀察細(xì)胞因子信號(hào)轉(zhuǎn)導(dǎo)抑制因子-3(SOCS3 mRNA)在兒童急性淋巴細(xì)胞白血病(ALL)中的表達(dá)水平,分析SOCS3 mRNA表達(dá)水平高低與兒童ALL疾病狀態(tài)、危險(xiǎn)度之間的關(guān)系,探討SOCS3 mRNA在兒童ALL疾病狀態(tài)和危險(xiǎn)度評(píng)估、靶向治療中的應(yīng)用。方法:采用SYBR Green熒光定量PCR的方法檢測(cè)45例新診斷ALL患兒初診時(shí)、誘導(dǎo)緩解期和13例正常兒童骨髓單個(gè)核細(xì)胞中SOCS3 mRNA表達(dá)水平;分別采用方差分析和秩和檢驗(yàn)的方法分析SOCS3 mRNA表達(dá)水平與ALL危險(xiǎn)度、臨床特點(diǎn)的關(guān)系。同時(shí)采用免疫熒光組織化學(xué)染色聯(lián)合激光共聚焦顯微技術(shù)測(cè)定ALL患兒骨髓單核細(xì)胞中SOCS3 mRNA的位置與表達(dá)量。結(jié)果:45例患兒初診時(shí)SOCS3mRNA表達(dá)水平明顯低于正常兒童(P0.05);誘導(dǎo)緩解期45例患兒的SOCS3 mRNA表達(dá)水平與正常兒童無(wú)顯著差異(P0.05);初診時(shí)中、高危組患兒SOCS3 mRNA表達(dá)水平高于低危組(P0.05);按照中位數(shù)法將45例患兒初診時(shí)SOCS3 mRNA表達(dá)水平分為高表達(dá)組和低表達(dá)組,比較2組患兒臨床特點(diǎn)發(fā)現(xiàn),SOCS3 mRNA高表達(dá)組具有更高的外周血白細(xì)胞計(jì)數(shù)、乳酸脫氫酶水平和預(yù)后不良基因。此外,2組在危險(xiǎn)度比較時(shí)SOCS3 mRNA高表達(dá)組危險(xiǎn)度更高。結(jié)論:SOCS3 mRNA在初診時(shí)下調(diào)而在疾病誘導(dǎo)緩解后恢復(fù)正常表達(dá),SOCS3可以作為評(píng)價(jià)疾病狀態(tài)的指標(biāo)。SOCS3 mRNA的高表達(dá)使ALL危險(xiǎn)度上調(diào),SOCS3 mRNA可以作為評(píng)價(jià)ALL危險(xiǎn)度的因子,通過(guò)調(diào)節(jié)SOCS3 mRNA表達(dá)水平治療ALL或許可以成為治療的新方向。
[Abstract]:Objective: to observe the expression of cytokine signal transduction inhibitor 3 (SOCS3 mRNA) in (ALL) of childhood acute lymphoblastic leukemia (AML), and to analyze the relationship between the expression of SOCS3 mRNA and the disease status and risk of ALL in children. To explore the application of SOCS3 mRNA in the assessment of disease status and risk of ALL in children. Methods: the expression of SOCS3 mRNA in bone marrow mononuclear cells of 45 newly diagnosed ALL children and 13 normal children was detected by SYBR Green fluorescence quantitative PCR. ANOVA and rank sum test were used to analyze the relationship between SOCS3 mRNA expression level and ALL risk and clinical characteristics. Immunofluorescence histochemical staining and laser confocal microscopy were used to detect the location and expression of SOCS3 mRNA in bone marrow monocytes of children with ALL. Results: the expression of SOCS3mRNA in 45 children was significantly lower than that in normal children (P0.05), but there was no significant difference in SOCS3 mRNA expression between 45 children and normal children (P0.05). In the first visit, the expression of SOCS3 mRNA in high risk group was higher than that in low risk group (P0.05). According to the median method, 45 children were divided into high expression group and low expression group according to the median method. Comparing the clinical characteristics of the two groups, it was found that the high expression group of SOCS3 mRNA had higher peripheral blood leukocyte count. Lactate dehydrogenase level and poor prognosis gene. In addition, the risk of high expression of SOCS3 mRNA was higher in the two groups than in the risk group. Conclusion: SOCS3 mRNA is down-regulated at first visit and returns to normal expression after disease induction and remission. SOCS3 can be used as an index to evaluate the disease state. The high expression of SOCS3 mRNA can increase the risk of ALL, and SOCS3 mRNA can be used as a risk factor to evaluate the risk of ALL. The treatment of ALL by regulating the level of SOCS3 mRNA expression may be a new direction of treatment.
【作者單位】： 安徽醫(yī)科大學(xué)第二附屬醫(yī)院兒科;
【分類號(hào)】：R733.71

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