【摘要】：目的建立SD幼鼠卵白蛋白(OVA)食物過敏(FA)模型；探討FA對幼鼠內(nèi)臟痛覺敏感性影響及結(jié)腸P物質(zhì)(SP)異常表達(dá)在FA幼鼠內(nèi)臟痛覺高敏形成中作用。 方法3周齡雌性SD大鼠20只，隨機(jī)分FA組和對照組(NS組)，，各10只。FA幼鼠通過低劑量OVA腹腔注射基礎(chǔ)致敏及高劑量OVA灌胃激發(fā)建立SD幼鼠OVA-FA；檢測血清OVA特異性IgE含量、觀察腸黏膜病理改變及嗜酸性粒細(xì)胞、肥大細(xì)胞浸潤情況進(jìn)行幼鼠FA模型評價(jià)。通過觀察兩組幼鼠在不同壓力結(jié)直腸擴(kuò)張(CRD)刺激后的腹外斜肌(EOMA)放電測量進(jìn)行內(nèi)臟痛覺敏感性評價(jià)。應(yīng)用免疫組織化學(xué)染色及計(jì)算機(jī)圖像分析系統(tǒng)對結(jié)腸SP進(jìn)行積分光密度值半定量分析。采用SPSS13.0軟件包進(jìn)行統(tǒng)計(jì)分析，α=0.05為顯著性檢驗(yàn)標(biāo)準(zhǔn)。 結(jié)果FA組幼鼠出現(xiàn)不同程度豎毛，毛發(fā)失去光澤，活動(dòng)減少或俯臥不動(dòng)，抓耳撓腮或躁動(dòng)不安及排不成形稀便；與對照組比較，F(xiàn)A組幼鼠血清OVA-IgE含量、空腸黏膜嗜酸性粒細(xì)胞和肥大細(xì)胞數(shù)及肥大細(xì)胞脫顆粒百分率均明顯升高，差別有統(tǒng)計(jì)學(xué)意義(P均＜0.01)。在CRD為0、15、30、45、60、75mmHg時(shí)，F(xiàn)A組腹外斜肌放電幅值(uV)分別為17.74±0.72、18.63±1.72、22.55±1.70、28.63±7.00、33.97±7.34、37.26±8.4，NS組分別為17.43±1.18、17.27±1.16、17.73±1.42、19.55±3.54、23.29±5.46、25.20±4.75。隨著CRD增加，腹外斜肌放電幅值較前明顯升高，CRD30、45、60、75mmHg時(shí)，兩組差別有統(tǒng)計(jì)學(xué)意義(P均＜0.01)。FA組、NS組幼鼠結(jié)腸SP積分光密度值分別為247.12±90.83、103.90±58.94，差別有統(tǒng)計(jì)學(xué)意義(P＜0.01)。 結(jié)論通過低劑量OVA腹腔注射基礎(chǔ)致敏及高劑量OVA灌胃激發(fā)可建立SD幼鼠FA模型。生命早期FA可導(dǎo)致幼鼠腹外斜肌放電幅值明顯升高并伴有結(jié)腸SP表達(dá)增加，出現(xiàn)慢性內(nèi)臟痛覺高敏感。生命早期FA導(dǎo)致的內(nèi)臟痛覺高敏感性發(fā)生可能與腸黏膜肥大細(xì)胞表達(dá)增多、脫顆粒及腸道SP的異常表達(dá)有關(guān)。
[Abstract]:Objective to establish the (FA) model of food allergy to ovalbumin (OVA) in young SD rats and to investigate the effect of FA on visceral pain sensitivity and the abnormal expression of substance P (SP) in colon during the formation of visceral hyperalgesia Gao Min in FA young rats. Methods Twenty 3-week-old female SD rats were randomly divided into FA group (n = 10) and control group (NS group, n = 10). SD young rats were sensitized by low dose OVA intraperitoneal injection and stimulated by high dose OVA. OVA-FA; was used to detect OVA IgE content in serum of SD young rats. The pathological changes of intestinal mucosa and the infiltration of eosinophils and mast cells were observed to evaluate the FA model of young rats. The visceral pain sensitivity was evaluated by observing the (EOMA) discharges of the oblique ventral muscles of the two groups after (CRD) stimulation under different pressures. Immunohistochemical staining and computer image analysis system were used for semi-quantitative analysis of integrated optical density (OSD) of colon SP. SPSS13.0 software package was used for statistical analysis, 偽 -0. 05 was the significant test standard. Results the young rats in FA group had different degrees of hair erecting, hair losing luster, decreased activity or prone immobility, scratching of ear, restlessness or restlessness and defecation, compared with control group, the content of serum OVA-IgE in FA group was higher than that in control group. The number of eosinophilic granulocytes and mast cells and the percentage of degranulation of mast cells in jejunal mucosa were significantly increased (P < 0.01). When the CRD was 0 ~ 15 ~ 30 ~ 45 ~ 60 ~ 75mmHg, the (uV) of the external oblique muscle in the FA group was 17.74 鹵0.72 ~ 18.63 鹵1.72t 22.55 鹵1.7028.63 鹵7.34 鹵37.97 鹵7.34 ~ 37.26 鹵8.4mm Hg, respectively, which was 17.43 鹵1.1817.27 鹵1.167.73 鹵1.42n 19.55 鹵3.523.29 鹵5.46n 25.20 鹵4.75 respectively. With the increase of CRD, the discharge amplitude of the oblique ventral muscle increased significantly (P < 0. 01). The SP optical density of colon in the FA group was 247.12 鹵90.83103.90 鹵58.94, respectively (P < 0. 01), and there was a significant difference between the two groups (P < 0. 01). Conclusion the FA model of young SD rats can be established by low dose intraperitoneal injection of OVA and high dose of OVA by intraperitoneal injection. Early FA could cause the amplitude of ventral obliquus muscle discharge and the expression of SP in colon increased, and the chronic visceral pain was hypersensitive. The high sensitivity of visceral pain induced by FA in early life may be related to the increased expression of mast cells in intestinal mucosa, degranulation and abnormal expression of intestinal SP.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R725.7;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊燕珍;吳斌;張睿;卓玲;陳兢芳;林國威;林春;;腹外斜肌放電測量在幼鼠內(nèi)臟痛覺高敏感模型評價(jià)中的應(yīng)用[J];中國當(dāng)代兒科雜志;2008年05期

2 李楓;;圖像分析中光密度參數(shù)物理意義的正確理解和使用[J];解剖學(xué)雜志;2009年02期

3 張世紅,趙晏;P物質(zhì)的免疫調(diào)節(jié)作用[J];生理科學(xué)進(jìn)展;2002年03期

4 陳曉敏;羅云;吳躍龍;江敏;;腸易激綜合征患者結(jié)腸黏膜辣椒素受體、P物質(zhì)和肥大細(xì)胞變化的研究[J];胃腸病學(xué);2010年11期

5 Hirotada Akiho;Eikichi Ihara;Kazuhiko Nakamura;;Low-grade inflammation plays a pivotal role in gastrointestinal dysfunction in irritable bowel syndrome[J];World Journal of Gastrointestinal Pathophysiology;2010年03期

6 王巍峰,楊云生,孫剛,彭麗華;腸易激綜合征大鼠P物質(zhì)能神經(jīng)通路的改變[J];世界華人消化雜志;2005年02期

7 李斐,黎海芪;金銀花水提物對卵清蛋白致敏小鼠的免疫調(diào)控作用[J];中華兒科雜志;2005年11期

8 姚明,楊建平,肖旺頻,矯勇軼,蔣星紅,印其章;大鼠結(jié)直腸擴(kuò)張內(nèi)臟痛模型痛閾的影響因素[J];中華麻醉學(xué)雜志;2004年07期

9 董文珠,李兆申,鄒多武,許國銘,鄒曉平,朱愛勇,尹寧,龔燕芳;腸易激綜合征患者腸黏膜肥大細(xì)胞與P物質(zhì)的相關(guān)性[J];中華內(nèi)科雜志;2003年09期

10 楊崇美;李延青;;剔除過敏食物治療腸易激綜合征的療效觀察[J];中華內(nèi)科雜志;2007年08期

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