【摘要】：目的探討β整合素家族成員在兒童急性T淋巴細(xì)胞白血病(T-ALL)的表達(dá)及臨床意義。方法收集22例初診T-ALL患兒和21例對照(非惡性血液病患者和骨髓移植供者)的骨髓標(biāo)本,采用實時熒光定量PCR方法,檢測β整合素家族各成員的m RNA表達(dá);并利用整合素抑制劑精氨酸-甘氨酸-天冬氨酸(RGD)肽作用于Jurkat細(xì)胞,采用CCK 8法和流式細(xì)胞術(shù)檢測Jurkat細(xì)胞生存率和凋亡情況。結(jié)果與對照組相比,T-ALL患兒的整合素β_2、β_3、β_5的m RNA表達(dá)水平顯著下調(diào)(P0.05)。在外周血白細(xì)胞計數(shù)100×10~9/L、第33天骨髓不緩解或MRD陽性的T-ALL患兒中,整合素β_3表達(dá)水平較高(P0.05);復(fù)發(fā)T-ALL患兒整合素β_5的表達(dá)高于無復(fù)發(fā)T-ALL患兒(P0.05)。整合素β3高表達(dá)T-ALL患兒的EFS率、OS率均低于β_3低表達(dá)者(P0.05)。與未處理組比較,RGD肽處理的Jurkat細(xì)胞生存率較低、凋亡率均高(P0.05)。結(jié)論β整合素可能通過影響細(xì)胞的增殖和凋亡而影響T-ALL的發(fā)生發(fā)展,整合素β_5的表達(dá)與T-ALL復(fù)發(fā)風(fēng)險密切相關(guān),整合素β_3的表達(dá)與T-ALL患兒治療反應(yīng)及預(yù)后密切相關(guān)。
[Abstract]:Objective to investigate the expression and clinical significance of 尾 integrin family members in children with acute T lymphocyte leukemia (T-ALL). Methods Bone marrow samples were collected from 22 newly diagnosed children with T-ALL and 21 controls (non-malignant hematological patients and bone marrow transplant donors). The expression of m RNA in 尾 integrin family members was detected by real-time quantitative PCR. Jurkat cells were treated with integrin inhibitor arginine glycine aspartic acid (RGD) peptide. The survival rate and apoptosis of Jurkat cells were detected by CCK 8 method and flow cytometry. Results compared with the control group, the expression of integrin 尾 2, 尾 3, 尾 5 in children with T-ALL was significantly down-regulated (P0.05). The expression of integrin 尾 3 was higher in peripheral blood leukocyte count of 100 脳 10 ~ (9 / L) / L, and the expression of integrin 尾 _ (5) in children with recurrent T-ALL was higher than that in children without recurrence of T-ALL (P0.05), but the expression of integrin 尾 _ (3) was higher in children with unremitting bone marrow or positive MRD on the 33rd day (P0.05). The EFS rate and OS rate of T-ALL patients with high expression of integrin 尾 3 were lower than those with low expression of integrin 尾 3 (P0.05). Compared with the untreated group, the survival rate of Jurkat cells treated with RGD peptide was lower and the apoptosis rate was higher (P0.05). Conclusion 尾 integrin may affect the development of T-ALL by affecting cell proliferation and apoptosis. The expression of integrin 尾 5 is closely related to the risk of recurrence of T-ALL. The expression of integrin 尾 3 is closely related to the therapeutic response and prognosis of children with T-ALL.
【作者單位】： 重慶醫(yī)科大學(xué)附屬兒童醫(yī)院血液腫瘤科/兒童發(fā)育疾病研究教育部重點實驗室/兒科學(xué)重慶市重點實驗室/兒童發(fā)育重大疾病國家國際科技合作基地;
【基金】：重慶市衛(wèi)生局課題(2015MSXM031)
【分類號】：R733.71
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本文編號：2197532