川崎病內(nèi)皮祖細胞數(shù)量和血栓前體蛋白水平的變化及丹參對其影響
發(fā)布時間：2018-07-21 21:09
【摘要】：目的 測定川崎病(Kawasaki disease,KD)患兒體內(nèi)內(nèi)皮祖細胞(endothelial progenitor cells,EPCs)數(shù)量及血栓前體蛋白(thrombus precursor protein,TpP)水平的變化,并研究加用丹參注射液治療后對川崎病患兒體內(nèi)EPCs數(shù)量及TpP水平變化是否有影響。探討川崎病內(nèi)皮功能、血流動力學變化及丹參對川崎病的治療作用。 方法 選取住院治療的20例KD患兒,其中男11例,女9例,為川崎病組,再隨機分成2組進行治療,其中1組僅給予傳統(tǒng)的丙種球蛋白2g/kg靜滴沖擊治療1次,阿司匹林30-50mg/kg/d,熱退3-5天后減量,潘生丁3-5mg/kg/d治療,選為非丹參組；另一組在此基礎(chǔ)上加用丹參注射液0.2-0.3ml/kg/次,1次／日,連用5天為丹參組。另選取門診體檢的10例健康小兒,其中男5例,女5例為對照組。川崎病組與對照組用藥前空腹用抗凝管采肘靜脈血3m1,分離出單核細胞和血漿,其中單核細胞經(jīng)處理后采用流式細胞儀檢測外周血EPCs的數(shù)量,血漿用雙抗體夾心固相酶聯(lián)免疫吸附法(ELI SA)檢測TpP水平,并對其結(jié)果進行統(tǒng)計學分析,比較兩組結(jié)果是否有統(tǒng)計學意義；丹參組和非丹參組與用藥5天后空腹采血測外周血EPCs數(shù)量和TpP水平,比較用丹參和不用丹參治療EPCs和TpP的變化是否具有統(tǒng)計學意義。 結(jié)果 ①川崎病組外周血中EPCs數(shù)量顯著低于對照組(P0.05)； ②川崎病組血漿中TpP水平顯著高于對照組(P0.05)； ③川崎病組經(jīng)治療后丹參組EPCs數(shù)量顯著高于非丹參組(P0.05)； ④川崎病組經(jīng)治療后丹參組TpP水平顯著低于非丹參組(P0.05)。 結(jié)論 川崎病組患兒測得外周血中EPCs數(shù)量較對照組明顯減少,說明川崎病患兒體內(nèi)生成EPCs減少或其消耗、破壞增多,提示存在內(nèi)皮功能損傷且內(nèi)皮修復及再生的能力下降；外周血TpP水平明顯升高,提示與川崎病患兒血液處于高凝狀態(tài)有關(guān),具有形成血栓或心肌梗塞的危險；經(jīng)治療后EPCs數(shù)量及TpP水平較前均明顯好轉(zhuǎn),提示川崎病經(jīng)治療后內(nèi)皮功能和血液高凝狀態(tài)均得到改善。丹參組EPCs數(shù)量較非丹參組明顯增高,而TpP水平較非丹參組明顯下降,提示加用丹參的治療方法對EPCs數(shù)量和TpP水平的改善較不加丹參更明顯,說明丹參具有增加川崎病患兒外周循環(huán)血EPCs數(shù)量及降低TpP水平的功能,從而發(fā)揮保護血管內(nèi)皮、促進內(nèi)皮修復和再生和抗凝等作用。祖國醫(yī)學將其歸結(jié)為活血化瘀功效。
[Abstract]:Objective to determine the number of endothelial progenitor cells (endothelial progenitor cells) and the level of thromboplastin (thrombus precursor protein (TP) in children with Kawasaki disease (KD). The effects of salvia miltiorrhiza injection on the number of EPCs and the level of TP in children with Kawasaki disease were studied. To investigate the changes of endothelial function and hemodynamics of Kawasaki disease and the therapeutic effect of Salvia miltiorrhiza on Kawasaki disease. Methods Twenty children with KD were randomly divided into two groups, including 11 males and 9 females, who were treated with Kawasaki disease. Among them, group 1 was treated with conventional intravenous infusion of gamma globulin (2g/kg) only once. Aspirin 30-50 mg / kg / d, reduced after 3 to 5 days of heat withdrawal, dipyridamole 3-5mg/kg/d was selected as non-salvia miltiorrhiza group, another group was treated with salvia miltiorrhiza injection 0.2-0.3ml/kg/ once a day for 5 days. In addition, 10 healthy children, including 5 males and 5 females, were selected as control group. In Kawasaki disease group and control group, 3ml blood was collected from elbow vein blood with anticoagulant tube on an empty stomach before treatment. Monocytes and plasma were isolated. Flow cytometry was used to detect the number of EPCs in peripheral blood. The plasma levels of TP were detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Eli SA), and the results were analyzed statistically. The number of EPCs and the level of TP in peripheral blood of Danshen group and non-salvia miltiorrhiza group were measured on an empty stomach after 5 days. The changes of EPCs and TpP were statistically significant between salvia miltiorrhiza group and non-salvia miltiorrhiza group. Results 1the number of EPCs in the peripheral blood of Kawasaki disease group was significantly lower than that in the control group (P0.05), and the plasma level of TP in Kawasaki disease group was significantly higher than that in the control group (P0.05). 3 the number of EPCs in the group of Kawasaki disease was significantly higher than that in the group without salvia miltiorrhiza (P0.05), and the level of TpP in the group of Kawasaki disease was significantly lower than that in the group without salvia miltiorrhiza (P0.05). Conclusion the number of EPCs in the peripheral blood of children with Kawasaki disease was significantly lower than that in the control group, which indicated that the EPCs production in children with Kawasaki disease was decreased or consumed, and the destruction was increased in children with Kawasaki disease. The results indicated that there were endothelial function damage and the ability of endothelial repair and regeneration decreased, and the level of TPP in peripheral blood was significantly increased, suggesting that the blood of children with Kawasaki disease was in a hypercoagulable state and was at risk of thrombosis or myocardial infarction. After treatment, the number of EPCs and the level of TP were significantly improved, suggesting that the endothelial function and blood hypercoagulability of Kawasaki disease were improved after treatment. The number of EPCs in the salvia miltiorrhiza group was significantly higher than that in the non-salvia miltiorrhiza group, while the level of TpP in the salvia miltiorrhiza group was significantly lower than that in the non-salvia miltiorrhiza group. These results suggest that salvia miltiorrhiza has the function of increasing the number of EPCs in peripheral circulation and decreasing the level of TP in the peripheral circulation of children with Kawasaki disease, so as to protect vascular endothelium, promote endothelial repair, regeneration and anticoagulation. Traditional Chinese medicine ascribes it to the effect of promoting blood circulation and removing blood stasis.
【學位授予單位】：湖北中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R725.4
本文編號：2136863
[Abstract]:Objective to determine the number of endothelial progenitor cells (endothelial progenitor cells) and the level of thromboplastin (thrombus precursor protein (TP) in children with Kawasaki disease (KD). The effects of salvia miltiorrhiza injection on the number of EPCs and the level of TP in children with Kawasaki disease were studied. To investigate the changes of endothelial function and hemodynamics of Kawasaki disease and the therapeutic effect of Salvia miltiorrhiza on Kawasaki disease. Methods Twenty children with KD were randomly divided into two groups, including 11 males and 9 females, who were treated with Kawasaki disease. Among them, group 1 was treated with conventional intravenous infusion of gamma globulin (2g/kg) only once. Aspirin 30-50 mg / kg / d, reduced after 3 to 5 days of heat withdrawal, dipyridamole 3-5mg/kg/d was selected as non-salvia miltiorrhiza group, another group was treated with salvia miltiorrhiza injection 0.2-0.3ml/kg/ once a day for 5 days. In addition, 10 healthy children, including 5 males and 5 females, were selected as control group. In Kawasaki disease group and control group, 3ml blood was collected from elbow vein blood with anticoagulant tube on an empty stomach before treatment. Monocytes and plasma were isolated. Flow cytometry was used to detect the number of EPCs in peripheral blood. The plasma levels of TP were detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Eli SA), and the results were analyzed statistically. The number of EPCs and the level of TP in peripheral blood of Danshen group and non-salvia miltiorrhiza group were measured on an empty stomach after 5 days. The changes of EPCs and TpP were statistically significant between salvia miltiorrhiza group and non-salvia miltiorrhiza group. Results 1the number of EPCs in the peripheral blood of Kawasaki disease group was significantly lower than that in the control group (P0.05), and the plasma level of TP in Kawasaki disease group was significantly higher than that in the control group (P0.05). 3 the number of EPCs in the group of Kawasaki disease was significantly higher than that in the group without salvia miltiorrhiza (P0.05), and the level of TpP in the group of Kawasaki disease was significantly lower than that in the group without salvia miltiorrhiza (P0.05). Conclusion the number of EPCs in the peripheral blood of children with Kawasaki disease was significantly lower than that in the control group, which indicated that the EPCs production in children with Kawasaki disease was decreased or consumed, and the destruction was increased in children with Kawasaki disease. The results indicated that there were endothelial function damage and the ability of endothelial repair and regeneration decreased, and the level of TPP in peripheral blood was significantly increased, suggesting that the blood of children with Kawasaki disease was in a hypercoagulable state and was at risk of thrombosis or myocardial infarction. After treatment, the number of EPCs and the level of TP were significantly improved, suggesting that the endothelial function and blood hypercoagulability of Kawasaki disease were improved after treatment. The number of EPCs in the salvia miltiorrhiza group was significantly higher than that in the non-salvia miltiorrhiza group, while the level of TpP in the salvia miltiorrhiza group was significantly lower than that in the non-salvia miltiorrhiza group. These results suggest that salvia miltiorrhiza has the function of increasing the number of EPCs in peripheral circulation and decreasing the level of TP in the peripheral circulation of children with Kawasaki disease, so as to protect vascular endothelium, promote endothelial repair, regeneration and anticoagulation. Traditional Chinese medicine ascribes it to the effect of promoting blood circulation and removing blood stasis.
【學位授予單位】：湖北中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R725.4
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