【摘要】：目的研究幼年期肺炎鏈球菌肺炎(S.pneumoniaepneumonia,S.pp)對成年期哮喘形成的影響。方法 3周齡雌性Balb/c小鼠隨機分為對照組(control)、幼年期肺炎鏈球菌肺炎組(Inf S.pp)、哮喘組(OVA)、幼年期肺炎鏈球菌肺炎哮喘組(Inf S.pp+OVA)。3周齡雌性Balb/c小鼠鼻腔滴注2×106cfu/ml肺炎鏈球菌25μl建立肺炎鏈球菌肺炎模型,成年期用OVA致敏激發(fā)Balb/c小鼠建立哮喘模型,最后1次激發(fā)完成24 h內(nèi)檢測小鼠氣道高反應(yīng)性,HE染色制作肺組織病理切片,觀察組織病理變化,檢測支氣管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中細胞總數(shù)、細胞分類計數(shù)及細胞因子水平,流式細胞術(shù)檢測小鼠肺組織中CD4~+T細胞水平。結(jié)果幼年期肺炎鏈球菌肺炎哮喘組肺組織炎癥細胞侵潤、氣道反應(yīng)性、BALF細胞總數(shù)、嗜酸性粒細胞和中性粒細胞及細胞因子IL-5、IL-13、IL-17水平顯著高于對照組(P0.05),IFN-γ、IL-10表達顯著降低(P0.05),肺組織內(nèi)Th2、Th17水平顯著高于對照組(P0.05),Foxp3~+Treg水平顯著降低(P0.01),Th1/Th2、Foxp3~+Treg/Th17比例明顯降低(P0.01)。幼年期肺炎鏈球菌肺炎哮喘組肺組織炎癥細胞侵潤、氣道高反應(yīng)性、BALF細胞分類計數(shù)及炎性因子水平、肺組織CD4~+T細胞水平與哮喘組比較無統(tǒng)計學(xué)差異。結(jié)論幼年期肺炎鏈球菌肺炎對成年期哮喘形成無顯著影響。
[Abstract]:Objective to study the effect of S. pneumoniae S.pp on adult asthma. Methods 3-week-old female Balb / c mice were randomly divided into control group (Inf S.pp), asthmatic group (OVA) and female Balb / c mice (Inf S.pp OVA), who were given intranasal drip of 2 脳 106cfu/ml Streptococcus pneumoniae 25 渭 l in nasal cavity. Establishment of Streptococcus pneumoniae pneumonia model, In adult, Balb / c mice were sensitized by OVA to establish asthma model. The lung tissue sections were made by HE staining of airway hyperresponsiveness within 24 hours after the last stimulation, and the histopathological changes were observed. The total number of cells, cell classification count and cytokine level in bronchoalveolar lavage fluid (bronchoalveolar lavage fluidd BALF) were measured. Flow cytometry was used to detect the level of CD4T cells in mouse lung tissue. Results the inflammatory cells of lung tissue and the total number of BALF cells of airway reactivity in asthmatic group of infant streptococcus pneumoniae pneumonia were infiltrated. The levels of IL-17 in eosinophils, neutrophils and cytokines were significantly higher than those in the control group (P0.05). The expression of IL-10 in the lung tissue was significantly lower than that in the control group (P0.05), and the level of Th2Th17 in lung tissue was significantly higher than that in the control group (P0.05). (P0.01) the proportion of Th1 / Th2Foxp3Treg-Th17 was significantly decreased (P0.01). There was no significant difference in inflammatory cell infiltration, BALF cell classification and inflammatory factor level between asthma group and asthma group, and the level of CD4T cells in lung tissue was not significantly different from that in asthma group. Conclusion Streptococcus pneumoniae pneumonia has no significant effect on the development of adult asthma.
【作者單位】： 重慶醫(yī)科大學(xué)附屬兒童醫(yī)院 兒童發(fā)育疾病研究教育部重點實驗室兒科學(xué)重慶市重點實驗室兒童發(fā)育重大疾病國際科技合作基地;重慶醫(yī)科大學(xué)附屬兒童醫(yī)院呼吸科;
【基金】：國家自然科學(xué)基金(81270086,81470222)
【分類號】：R725.6

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