【摘要】：目的探討IL-2(rs6822844)及IL-2Rα(rs2104286)基因多態(tài)性和漢族兒童支氣管哮喘發(fā)生的關(guān)聯(lián)。方法隨機(jī)選取144例支氣管哮喘患兒為病例組,228例健康兒童為對照組。用實(shí)時熒光定量聚合酶鏈反應(yīng)(q RT-PCR)直接測序鑒定各位點(diǎn)基因型;同時采用q RT-PCR檢測對照組IL-2Rα各基因型外周血IL-2Rα信使核糖核酸(m RNA)相對表達(dá)量,采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測各基因型血漿s IL-2Rα水平。結(jié)果IL-2Rα(rs2104286)位點(diǎn)等位基因A在病例組分布頻率(86.8%)高于對照組(79.4%)(OR=1.708,95%CI:1.113,2.629,P=0.010),病例組中AA基因型分布頻率(75.0%)高于對照組(62.3%)(OR=1.745,95%CI:1.058,2.884,P=0.021);未發(fā)現(xiàn)IL-2(rs6822844)位點(diǎn)有多態(tài)性分布。對照組中IL-2Rα(rs2104286)位點(diǎn)AA基因型個體外周血IL-2Rαm RNA相對表達(dá)量以及血漿s IL-2Rα水平均高于AG基因型(Z=-2.029和-2.361,P=0.043和0.018)。結(jié)論 IL-2Rα(rs2104286)基因多態(tài)性可能與漢族兒童支氣管哮喘發(fā)生相關(guān),而且該位點(diǎn)基因型可影響其轉(zhuǎn)錄及表達(dá)。
[Abstract]:Objective to investigate the association of interleukin-2 (rs6822844) and IL-2R 偽 (rs2104286) gene polymorphisms with bronchial asthma in children of Han nationality. Methods 144 children with bronchial asthma were randomly selected as case group and 228 healthy children as control group. The locus genotypes were identified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the relative expression of IL-2R 偽 messenger ribonucleic acid (mRNA) in peripheral blood of the control group was detected by qRT-PCR. Enzyme linked immunosorbent assay (Elisa) was used to detect the levels of plasma sIL-2R 偽 in all genotypes. Results the distribution frequency of IL-2R 偽 (rs2104286) allele A in the case group (86.8%) was higher than that in the control group (79.4%) (OR1. 708%). The distribution frequency of AA genotype in the case group (75.0%) was higher than that in the control group (62.3%). There was no polymorphic distribution of IL-2 (rs6822844) locus. The relative expression of IL-2R 偽 mRNA in peripheral blood and the level of plasma sIL-2R 偽 in AA genotype at IL-2R 偽 (rs2104286) locus were significantly higher than those in AG genotype (Z ~ (-2.029) and -2.361) (0.043 and 0.018). Conclusion the polymorphism of IL-2R 偽 (rs2104286) gene may be associated with bronchial asthma in Han children, and the genotype of IL-2R 偽 (rs2104286) gene may affect its transcription and expression.
【作者單位】： 浙江省金華市中醫(yī)醫(yī)院檢驗(yàn)科;浙江省杭州市第一人民醫(yī)院檢驗(yàn)科;
【分類號】：R725.6

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