本文選題：缺氧缺血性腦損傷 + 缺氧誘導因子-α　； 參考：《中西醫(yī)結合心腦血管病雜志》2012年08期

【摘要】：目的探討缺氧誘導因子-1α(HIF-1α)及其抑制劑2-甲氧基雌二醇(2ME2)對新生大鼠缺氧缺血性腦損傷時神經(jīng)細胞凋亡的影響。方法將120只新生7d齡Wistar大鼠隨機分為假手術組(Sham組,n=8)、缺氧缺血模型組(HIBD組,n=56)、2ME2干預組(2ME2組,n=56),后兩組采用Rice法建立模型后,根據(jù)斷頭取腦的時間不同又分為3h、6h、12h、24h、48h、72h和7d7個亞組。應用HE染色觀察腦組織的病理變化,免疫組化技術檢測HIF-1α、Bax、Bcl-2的表達,TUNEL法計數(shù)腦細胞凋亡。結果 HE染色顯示2ME2干預后腦組織的損傷減輕;免疫組化顯示:HIF-1α在HIBD組于模型制備后3h升高,12h達高峰,之后下降,2ME2組表達趨勢和HIBD組相同,表達水平顯著下降。與HIBD組比較,2ME2組各時間點的Bcl-2表達顯著升高,Bax表達顯著降低,TUNEL標記的陽性細胞數(shù)明顯減少。結論在新生大鼠缺氧缺血急性期使用2ME2抑制HIF-1α的表達,引起B(yǎng)ax/Bcl-2表達量比值降低,凋亡細胞數(shù)目減少,改善腦損傷。
[Abstract]:Objective to investigate the effects of hypoxia inducible factor-1 偽 (HIF-1 偽) and its inhibitor 2-methoxyestradiol (2ME2) on neuronal apoptosis in neonatal rats with hypoxic-ischemic brain injury. Methods 120 7-day-old Wistar rats were randomly divided into sham-operated group (Sham group), hypoxic-ischemic model group (HIBD group) and 2ME2 intervention group (2ME2 group). The pathological changes of brain tissue were observed by HE staining and apoptosis of brain cells was counted by Tunel method. Results HE staining showed that the damage of brain tissue was alleviated after 2ME2 intervention, and the expression level of WHIF-1 偽 in HIBD group increased to a peak at 3 h after HIBD and reached the peak at 12 h after HIBD, and the expression trend of HIF-1 偽 in HIBD group was the same as that in HIBD group, and the expression level was significantly decreased in HIBD group. Compared with HIBD group, the expression of Bcl-2 was significantly increased in group 鈪，

本文編號：2087923