本文選題：黃芩苷 + 缺氧缺血性腦損傷　； 參考：《南昌大學》2012年碩士論文

【摘要】：目的： 1．研究黃芩苷(baicalin, BC)對缺氧缺血性腦損傷(hypoxic-ischemic braindamage, HIBD)新生大鼠腦細胞Fas、FasL表達的影響。 2．比較黃芩苷與神經(jīng)生長因子(nerve growth factor, NGF)對新生大鼠缺氧缺血性腦損傷的神經(jīng)保護作用。 方法： 1．實驗分組：128只7日齡SD大鼠隨機分為四組(每組32只)：假手術對照組(Sham組)；缺氧缺血性腦損傷對照組(HIBD組)；神經(jīng)生長因子對照組(NGF組)；黃芩苷治療組(BC組)。 2．模型制作及藥物干預：參照Rice法制成HIBD模型，其中Sham組僅游離出左頸總動脈，不結扎，不缺氧。NGF組于缺氧缺血(hypoxia-ischemia, HI)、復氧恢復處理后，立刻給予腹腔注射NGF注射液50μg/kg，每天1次，連用3天；BC組于HI、復氧恢復處理后，立刻給予腹腔注射BC注射液16mg/kg，每天1次，連用3天；Sham組和HIBD組每日腹腔注射生理鹽水10ml/kg，連用3天。 3．觀測指標：觀察新生大鼠行為改變、存活率和體重增長；各組均于HI后24小時、48小時、72小時、7天四個時間點(每個時間點各8只)分批取腦組織，HE染色后光鏡下觀察受損腦組織病理形態(tài)改變；S-P免疫組織化學方法測定腦細胞Fas、FasL表達。 結果： 1．行為改變：實驗大鼠在HI后約30分鐘開始出現(xiàn)倦怠，嗜睡，肌張力異常等不同程度的腦損傷癥狀，復氧后可見大鼠向結扎側旋轉。 2．存活率：Sham組實驗大鼠無死亡，，存活率為100%(32/32)；HIBD組死亡5只，存活率為84.38%(27/32)；NGF組死亡2只，存活率為93.75%(30/32)，BC組死亡3只，存活率為90.63%(29/32)。各組間存活率比較，差異無統(tǒng)計學意義(P0.05)。 3．7天體重增長：HIBD組大鼠體重增長最慢，7天體重增長值明顯小于其他三組(P0.05)，BC組與NGF組體重增長相近，兩組之間比較，差異無統(tǒng)計學意義(P0.05)，但顯著落后于Sham組(P0.01)。 4．腦組織病理形態(tài)學改變：Sham組大鼠腦細胞結構正常，排列整齊；HIBD組可見腦細胞排列紊亂，細胞水腫明顯，部分細胞出現(xiàn)壞死，呈空泡樣改變(HI后48小時最為顯著)；相同時間點：BC組和NGF組較HIBD組上述改變均明顯減輕，而且兩者改變相近。 5．腦細胞Fas、FasL表達：Sham組腦細胞可見少量Fas、FasL表達，各時間點差異無統(tǒng)計學意義(P0.05)；其余各組HI后24小時出現(xiàn)Fas、FasL表達，以HI后48小時最明顯，之后逐漸減少，持續(xù)至7天仍顯著高于Sham組，各時間點之間比較，差異有統(tǒng)計學意義(P0.01)；相同時間點：NGF組和BC組之間Fas、FasL表達比較，差異無統(tǒng)計學意義(P0.05)。 6．Fas與FasL相關性分析：新生大鼠HIBD腦細胞Fas表達和FasL表達之間存在直線相關關系(r=0.863，P=0.0000.01)。 結論： 1．新生大鼠HIBD腦細胞Fas、FasL表達明顯增加，以HI后48小時最為明顯，之后逐漸減少，持續(xù)至HI后7天仍可見陽性表達，并且正常新生大鼠腦細胞也可見少量Fas、FasL表達。 2．黃芩苷可以通過減少新生大鼠HIBD腦細胞Fas、FasL表達，抑制細胞凋亡，從而發(fā)揮腦神經(jīng)保護作用。 3．黃芩苷對新生大鼠HIBD的神經(jīng)保護作用與神經(jīng)生長因子相近，有可能替代神經(jīng)生長因子成為臨床上治療新生兒HIBD的一種新藥物。
[Abstract]:Purpose :

1 . To study the effects of baicalin ( BC ) on the expression of Fas and FasL in brain cells of neonatal rats with hypoxic - ischemic brain damage ( HIBD ) .

2 . To compare the protective effects of baicalin and nerve growth factor ( NGF ) on hypoxic - ischemic brain injury in neonatal rats .

Method :

1 . Experimental group : 128 SD rats were randomly divided into four groups ( 32 in each group ) : sham operation control group ( Sham group ) ;
hypoxic - ischemic brain injury control group ( HIBD group ) ;
Nerve growth factor control group ( NGF group ) ;
baicalin treatment group ( BC group ) .

2 . Model production and drug intervention : The HIBD model was established by reference to Rice method , in which Sham group only free left common carotid artery , no ligation , no hypoxia . NGF group was injected intraperitoneally with 50 渭g / kg NGF injection once a day for 3 days .
BC group was injected with the BC injection at 16 mg / kg once a day for 3 days .
Sham group and HIBD group were injected with normal saline ( 10ml / kg ) daily for 3 days .

3 . Observation index : observe the behavior change , survival rate and body weight gain of neonatal rats ;
The pathological changes of the injured brain tissues were observed under light microscope at 24 hours , 48 hours , 72 hours and 7 days after HI ( 8 rats at each time point ) .
The expression of Fas and FasL in brain cells was measured by S - P immunohistochemical method .

Results :

1 . Behavior change : After HI , the rats began to suffer from various degrees of brain injury , such as lassitude , drowsiness , abnormal muscle tension and so on , and the rats were seen to rotate to the ligation side after hyperbaric oxygenation .

2 . Survival rate : The survival rate was 100 % ( 32 / 32 ) in Sham group .
The survival rate of HIBD group was 84.38 % ( 27 / 32 ) .
The survival rate was 93.75 % ( 30 / 32 ) and the survival rate was 90.63 % ( 29 / 32 ) .

3 . 7 - day body weight gain : the weight gain of the HIBD group was the slowest , and the body weight gain value was significantly lower than that in other three groups ( P0.05 ) . The weight gain of the BC group was similar to that of the NGF group , but the difference was not statistically significant ( P0.05 ) , but the difference was significantly lower than Sham group ( P0.01 ) .

4 . Pathological changes of brain tissue : The brain cells of Sham group were normal and arranged orderly ;
In HIBD group , the brain cells were disordered , cell edema was obvious , some cells appeared necrosis and vacuolar change ( 48 hours after HI was the most significant ) ;
At the same time : BC group and NGF group were significantly lighter than HIBD group , and the changes were similar .

5 . The expression of Fas and FasL in brain cells showed that the expression of Fas and FasL in brain cells of Sham group was not significant ( P0.05 ) .
The expression of Fas and FasL appeared 24 hours after HI , and the expression of Fas and FasL was most obvious at 48 hours after HI . After HI , the expression of Fas and FasL was significantly higher than Sham group after HI , and the difference was significant ( P0.01 ) .
At the same time point : the expression of Fas and FasL between NGF group and BC group was not significant ( P0.05 ) .

6 . Fas and FasL correlation analysis : There was a linear correlation between Fas expression and FasL expression in neonatal rat HIBD brain cells ( r = 0.863 , P = 0.0000 . 01 ) .

Conclusion :

1 . The expression of Fas and FasL in HIBD brain cells was significantly increased in neonatal rats . After HI , the expression of Fas and FasL was gradually decreased and the expression of Fas and FasL was observed in normal neonatal rat brain cells .

2 . baicalin can inhibit the expression of Fas and FasL in HIBD brain cells of neonatal rats , inhibit the apoptosis of cells , and play the role of neuroprotection .

3 . The neuroprotection effect of baicalin on HIBD in neonatal rats is similar to that of nerve growth factor , and it is possible to replace nerve growth factor as a new drug for clinical treatment of HIBD in neonatal rats .
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R722.1

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相關期刊論文 前10條

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本文編號：2069462