本文選題：兒童 + 急性白血病��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討急性白血病(acute leukemia,AL)患兒初步診斷時和化療完全緩解(complete remission,CR)后血清中白介素2(interleukin-2,IL-2)、白介素10(interleukin-10,IL-10)含量的變化。方法:收集確診為急性淋巴細胞白血病(acute lymphoblastic leukemia,ALL)和急性髓細胞性白血病(acute myelocytic leukemia,AML)且經(jīng)過化療獲得完全緩解的患兒分別30例、13例及健康兒童20例,采用流式細胞術(shù)(flow cytometry method,FCM)測量初診組、CR組、對照組血清中IL-2、IL-10的值,比較化療前后及ALL組和AML組之間這兩種細胞因子含量的變化,并分別與健康對照組做對比。結(jié)果:1.ALL組初診1組IL-2水平(3.59±1.18)pg/ml與對照組IL-2水平(6.22±2.03)pg/ml相比較明顯下降(P0.001),初診1組IL-10的水平(15.88±4.73)pg/ml與正常兒童IL-10的水平(5.69±3.44)pg/ml相比較則顯著升高(P0.001)。隨著化療的進行IL-2水平(4.78±1.57)pg/ml明顯上升(P=0.005),IL-10水平(8.14±2.82)pg/ml顯著下降(P0.001),都未達到正常兒童水平(P=0.034和P=0.036)。差異均有統(tǒng)計學(xué)意義(P0.05)。2.AML組初診2組患兒IL-2水平(2.43±1.61)pg/ml與正常對照組兒童IL-2水平(6.22±2.03)pg/ml相比較明顯下降(P0.001),初診2組IL-10的水平(35.21±13.19)pg/ml與正常兒童IL-10的水平(5.69±3.44)pg/ml相比較則顯著升高(P0.001)。隨著化療的進行IL-2水平(4.24±1.93)pg/ml明顯上升(P=0.048),IL-10水平(12.06±3.81)pg/ml顯著下降(P0.001),都未達到正常兒童水平(P=0.026和P0.001)。差異均有統(tǒng)計學(xué)意義(P0.05)。3.ALL組和AML組的比較初診時和CR后ALL和AML患兒IL-2水平相比較差別不大(P=0.165和P=0.932);差異無統(tǒng)計學(xué)意義(P0.05)。而AML患兒IL-10水平明顯高于ALL患兒水平(P=0.001和P=0.022),差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.IL-2和IL-10含量的變化在AL患兒的發(fā)病及病情進展中至關(guān)重要。2.AL患兒體內(nèi)的免疫功能狀態(tài)可能隨著IL-2和IL-10水平的起伏而不斷變化,這兩種細胞因子可作為病情評估的指標。3.AML患兒IL-10含量高與其預(yù)后差密切相關(guān)。
[Abstract]:Objective: to investigate the changes of serum interleukin-2 (IL-2) and interleukin-10 (IL-10) levels in children with acute leukemia (AL) at the time of initial diagnosis and after complete remission of chemotherapy (complete remissions). Methods: 30 cases of acute lymphoblastic leukemia (acute lymphoblastic leukemiaall) and 30 cases of acute myeloid leukemia (acute myelocytic leukemia all) and 20 healthy children with complete remission after chemotherapy were collected. Flow cytometry (FCM) was used to measure the level of IL-2 / IL-10 in the serum of CR group and control group. The changes of cytokines before and after chemotherapy and between all group and AML group were compared, and compared with those in healthy control group. Results the level of IL-2 in all group (3.59 鹵1.18) pg/ml was significantly lower than that in control group (6.22 鹵2.03) pg/ml (P0.001), and the level of IL-10 in newly diagnosed group (15.88 鹵4.73) pg/ml was significantly higher than that in normal children (5.69 鹵3.44) pg/ml (P0.001). The level of IL-2 (4.78 鹵1.57) pg/ml increased significantly (P0. 005) and the level of IL-10 (8. 14 鹵2. 82) pg/ml decreased significantly (P0. 001), and did not reach the level of normal children (P0. 034 and P0. 036). The level of IL-2 in newly diagnosed children in AML group (2.43 鹵1.61) was significantly lower than that in normal control group (6.22 鹵2.03) pg/ml (P0.001). The level of IL-10 pg/ml in newly diagnosed group (35.21 鹵13.19) was significantly higher than that in normal children (5.69 鹵3.44) pg/ml (P0.001). The level of IL-2 (4.24 鹵1.93) pg/ml increased significantly (P0. 048) and the level of IL-10 (12. 06 鹵3. 81) pg/ml decreased significantly (P0. 001), and did not reach the level of normal children (P0. 026 and P0. 001). The difference between all group and AML group was statistically significant (P0.05). 3. There was no significant difference in IL-2 level between all and AML children at first diagnosis and after CR (P0. 165 and P0. 932), but the difference was not statistically significant (P0.05). The level of IL-10 in AML children was significantly higher than that in all children (P0. 001 and P0. 022), and the difference was statistically significant (P0.05). Conclusion the changes of IL-2 and IL-10 levels are very important in the pathogenesis and progression of children with AL. 2.The immune function of children with AL may change with the fluctuation of IL-2 and IL-10 levels. These two cytokines can be used as an index to evaluate the condition. 3. The high level of IL-10 is closely related to poor prognosis in children with AML.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R733.71

【參考文獻】

相關(guān)期刊論文 前10條

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本文編號：2058890