本文選題：人腺病毒 + 病毒分離　； 參考：《中國人獸共患病學(xué)報(bào)》2017年08期

【摘要】：目的對(duì)一起幼兒園聚集性發(fā)熱事件開展病原學(xué)調(diào)查,為疫情處置提供依據(jù)。方法采集部分患者呼吸道樣品,應(yīng)用real-time RT-PCR法篩查,腺病毒核酸陽性樣品接種HEp-2細(xì)胞系,PCR擴(kuò)增部分腺病毒Hexon高變區(qū)基因片段并測序,確定病毒型別并進(jìn)行變異分析。結(jié)果從10份咽拭子標(biāo)本中檢測到7份腺病毒核酸陽性。經(jīng)分離培養(yǎng)得到7株人腺病毒毒株。系統(tǒng)進(jìn)化分析確定均為人腺病毒7型。推導(dǎo)的氨基酸序列分析顯示,與近年來國內(nèi)外流行株相比,分離株的Hexon蛋白高變區(qū)無氨基酸位點(diǎn)突變。結(jié)論結(jié)合病例臨床表現(xiàn)以及流行病學(xué)調(diào)查結(jié)果,此事件證實(shí)系人腺病毒7型引起的急性呼吸道感染聚集性事件。
[Abstract]:Objective to investigate the etiology of an event of agglomeration fever in kindergarten, and to provide evidence for the treatment of epidemic situation. Methods the respiratory tract samples of some patients were screened by real-time RT-PCR. The Hexon hypervariable region gene fragment of adenovirus was amplified and sequenced in HEp-2 cell line. The type of Hexon gene was determined and the mutation was analyzed. Results 7 cases of adenovirus nucleic acid were detected from 10 pharyngeal swabs. Seven human adenovirus strains were isolated and cultured. Phylogenetic analysis confirmed that all of them were human adenovirus 7. The deduced amino acid sequence analysis showed that there was no amino acid mutation in the high variable region of Hexon protein compared with the prevalent strains at home and abroad in recent years. Conclusion combined with the clinical manifestation and epidemiological investigation, this event is confirmed to be a clustering event of acute respiratory tract infection caused by human adenovirus type 7.
【作者單位】： 福建省疾病預(yù)防控制中心;福州市疾病預(yù)防控制中心;福建省人獸共患病重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家高技術(shù)研究計(jì)劃(No.2011AA02A114)~~
【分類號(hào)】：R440;R725.1

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