本文選題：表沒食子兒茶素沒食子酸酯 + 腸缺血再灌注損傷　； 參考：《重慶醫(yī)學(xué)》2017年20期

【摘要】：目的探討表沒食子兒茶素沒食子酸酯(EGCG)對(duì)大鼠腸缺血再灌注損傷的影響及其作用機(jī)制。方法 40只SD大鼠均分為4組:假手術(shù)組(Sham組)、腸道缺血再灌注損傷組(IRI組)、EGCG預(yù)處理組(EGCG組)和Wnt激動(dòng)劑HLY78組(Wnt-Ag組)。IRI、EGCG和Wnt-Ag組無損傷血管夾夾閉腸系膜上動(dòng)脈(SMA)45min構(gòu)建腸缺血再灌注損傷模型,Sham組只做假手術(shù)處理。EGCG組在缺血前45 min腹腔注射EGCG(50 mg/kg),Wnt-Ag組在缺血前45 min腹腔注射EGCG(50mg/kg)+Wnt-Ag(5mg/kg),Sham組和IRI組腹腔注射等量生理鹽水。再灌注4h后HE染色觀察腸組織病理形態(tài);免疫組織化學(xué)檢測(cè)細(xì)胞凋亡;RT-PCR和ELISA檢測(cè)腸道和血清腫瘤壞死因子α(TNF-α)、白細(xì)胞介素6(IL-6)、白細(xì)胞介素1(IL-1)表達(dá),Western blot法檢測(cè)腸組織Wnt、β-catenin、p53、Bax和BCL-2表達(dá)。結(jié)果與Sham組相比,IRI組IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、細(xì)胞凋亡和腸道病理改變明顯增加,而BCL-2表達(dá)明顯減少。與IRI組相比,EGCG組IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、細(xì)胞凋亡和腸道病理改變明顯減少,而BCL-2表達(dá)明顯增加。與EGCG組相比,Wnt-Ag組IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、細(xì)胞凋亡和腸道病理改變明顯增加,而BCL-2表達(dá)明顯減少。結(jié)論 EGCG可通過抑制炎癥和凋亡而減輕腸缺血再灌注損傷,這種保護(hù)作用可能是通過抑制Wnt/β-catenin信號(hào)途徑介導(dǎo)。
[Abstract]:Objective to investigate the effects of epigallocatechin gallate (EGCG) on intestinal ischemia-reperfusion injury in rats and its mechanism. Methods Forty SD rats were divided into four groups: sham-operated group (Sham group), intestinal ischemia-reperfusion injury group (IRI group) and Wnt agonist HLY78 group (HLY78 group). The model of intestinal ischemia-reperfusion injury in Sham group was treated with sham-operation only. EGCG(50 mg / kg Wnt-Ag group was injected intraperitoneally with EGCG(50 mg / kg Wnt-Ag group at 45 min before ischemia. Wnt-Ag5 mg / kg + Sham group and IRI group were intraperitoneally injected with the same amount of normal saline. The histopathology of intestine was observed by HE staining for 4 h after reperfusion, and the expressions of Wnt, 尾 -catenin p53 Bax and BCL-2 in intestinal tissue were detected by immunohistochemistry, RT-PCR and ELISA were used to detect the expression of TNF- 偽, IL-6, IL-6 and IL-1in intestinal tissue. Results compared with Sham group, IL-6 and IL-1TNF- 偽 TNF- 偽 Wnt, 尾 -catenin p53 Bax. apoptosis and intestinal pathological changes were significantly increased, while the expression of BCL-2 was significantly decreased. Compared with IRI group, IL-6 and IL-1TNF- 偽 Wnt, 尾 -catenin p53 Bax. apoptosis and intestinal pathological changes were significantly decreased, while the expression of BCL-2 was significantly increased in EGCG group. Compared with EGCG group, the expression of IL-6 and IL-1TNF- 偽 in Wnt-Ag group was significantly higher than that in Wnt-Ag group, and the expression of TNF- 偽, 尾 -cateninin p53, Bax. apoptosis and intestinal pathological changes were significantly increased, while the expression of BCL-2 was significantly decreased in Wnt-Ag group. Conclusion EGCG can attenuate intestinal ischemia-reperfusion injury by inhibiting inflammation and apoptosis, which may be mediated by inhibiting Wnt/ 尾 -catenin signaling pathway.
【作者單位】： 四川大學(xué)華西第四醫(yī)院重癥監(jiān)護(hù)室;中航工業(yè)363醫(yī)院放療科;
【分類號(hào)】：R574

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 Jing-yao PANG;Kui-jun ZHAO;Jia-bo WANG;Zhi-jie MA;Xiao-he XIAO;;綠茶多酚表沒食子兒茶素沒食子酸酯(EGCG)體外對(duì)抗乙肝病毒復(fù)制的能力(英文)[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2014年06期

2 冉志華;陳遲;蕭樹東;;EGCG對(duì)乙酸誘導(dǎo)大鼠結(jié)腸炎的治療作用及抗氧化機(jī)制[J];世界華人消化雜志;2007年04期

3 嚴(yán)煜鈞;劉仲華;林勇;黃建安;彭彬;蔣華軍;;茶葉中EGCG對(duì)非酒精性脂肪肝大鼠的調(diào)脂保肝作用研究[J];茶葉科學(xué);2014年03期

4 韋遠(yuǎn)歡;施文祥;任源;;EGCG對(duì)酒精性肝病小鼠肝臟鐵調(diào)素表達(dá)影響[J];中國公共衛(wèi)生;2014年03期

5 甄茂川;王效民;尹震宇;汪謙;劉平果;吳國洋;俞可克;李崗山;;EGCG對(duì)肝纖維化大鼠TGF-β1和CTGF表達(dá)的影響[J];世界華人消化雜志;2008年34期

6 楊衛(wèi)振;脫紅芳;彭彥輝;張萬星;暴雷;楊月卿;;EGCG對(duì)雨蛙素誘導(dǎo)的小鼠急性胰腺炎的治療作用[J];實(shí)用醫(yī)學(xué)雜志;2012年24期

相關(guān)會(huì)議論文 前2條

1 孟敏;李延青;閆明先;任洪波;寇毅;;EGCG抗大鼠胰腺纖維化及其機(jī)制研究[A];中華醫(yī)學(xué)會(huì)第七次全國消化病學(xué)術(shù)會(huì)議論文匯編（下冊(cè)）[C];2007年

2 周曉蓉;龔作炯;袁光金;孫小梅;;茶多酚及EGCG對(duì)實(shí)驗(yàn)性酒精性肝病大鼠肝臟的保護(hù)作用及機(jī)制研究[A];中華中醫(yī)藥學(xué)會(huì)第十二屆內(nèi)科肝膽病學(xué)術(shù)會(huì)議暨第四次國家中醫(yī)肝病重點(diǎn)�？茀f(xié)作組學(xué)術(shù)會(huì)議論文匯編[C];2006年

相關(guān)碩士學(xué)位論文 前3條

1 賈亮;EGCG和PC-B2對(duì)AFB_1致肝細(xì)胞DNA損傷的保護(hù)作用與機(jī)制研究[D];廣西醫(yī)科大學(xué);2015年

2 于淑靜;天然產(chǎn)物EGCG對(duì)TP致免疫性肝損傷的保護(hù)作用及其機(jī)制研究[D];重慶醫(yī)科大學(xué);2016年

3 蔣華軍;EGCG干預(yù)非酒精性脂肪肝大鼠代謝組學(xué)研究[D];湖南農(nóng)業(yè)大學(xué);2013年

，

本文編號(hào)：1986007