發(fā)布時(shí)間：2018-06-01 23:24

  本文選題：伯基特淋巴瘤 + EB病毒　； 參考：《南昌大學(xué)》2014年碩士論文

【摘要】：目的： 探討miR-127、miR-155和miR-200a在EB病毒（Epstein-Barr virus,EBV）感染相關(guān)的兒童散發(fā)性伯基特淋巴瘤(Burkitt's lymphoma,BL)中的表達(dá)及意義。 方法： 1、收集2005～2012年江西省兒童醫(yī)院病理科外科手術(shù)切除的40例BL標(biāo)本及15例慢性扁桃體炎標(biāo)本。分析其臨床病理特征及隨訪資料，制備組織芯片，運(yùn)用原位雜交技術(shù)對(duì)標(biāo)本進(jìn)行EBV基因檢測；根據(jù)EBV基因檢測結(jié)果將BL分為EBV感染陰性組、EBV感染陽性組和正常對(duì)照組。 2、運(yùn)用qRT-PCR法，對(duì)已分組的40例BL和15例慢性扁桃體炎石蠟標(biāo)本進(jìn)行miR-127、 miR-155和miR-200a表達(dá)的檢測。 3、培養(yǎng)BL細(xì)胞株Raji（EBV陽性）和Ramos（EBV陰性），運(yùn)用qRT-PCR法對(duì)細(xì)胞株中miR-127、 miR-155和miR-200a的表達(dá)進(jìn)行檢測。 4、運(yùn)用熒光原位雜交（fluorescence in-situ hybridization,FISH）技術(shù)檢測40例BL石蠟組織及BL細(xì)胞株中c-myc基因的異常情況。 5、使用13.0統(tǒng)計(jì)軟件包對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行分析,計(jì)量資料采用“平均值±標(biāo)準(zhǔn)差”,計(jì)數(shù)資料采用t檢驗(yàn)。P0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果： 1、40例BL病例中16例EBER陽性（40%），其中2例（12.5%）位于腸系膜淋巴結(jié)、4例（25%）位于回盲部腸組織、10例（62.5%）位于腸系膜+回盲部；24例EBER陰性（60%），其中2例(8.33%)位于腸系膜淋巴結(jié)、6例(25%)位于回盲部腸組織、16例(66.67%)位于腸系膜+回盲部。15例扁桃體組織EBER均為陰性（100%） 2、40例BL中38例c-myc基因斷裂陽性（95%），其中EBER陰性23例（60.53％），EBER陽性15例（39.47%）；2例c-myc基因信號(hào)正常（5％），EBER陽性和EBER陰性各1例（各50%）。Raji細(xì)胞株c-myc基因斷裂陽性，Ramos細(xì)胞株基因信號(hào)正常。 3、與EBER陰性的BL病例及扁桃體組織比較，EBER陽性的BL病例miR-127表達(dá)上調(diào)（P㩳0.05），差異有統(tǒng)計(jì)學(xué)意義。Raji細(xì)胞株和Ramos細(xì)胞株相比，Raji細(xì)胞株中miR-127表達(dá)上調(diào)（P㩳0.05）。與EBER陰性的BL病例比較，，miR-155在EBER陽性的BL病例中表達(dá)上調(diào)，但無顯著差異性（P㧐0.05），EBER陽性BL的病例與扁桃體組織相比表達(dá)上調(diào)（P㩳0.05），差異有統(tǒng)計(jì)學(xué)意義。Raji和Ramos比較，在Raji細(xì)胞株中miR-155的表達(dá)上調(diào)（P㩳0.05）。miR-200a的表達(dá)在EBV陽性的BL相比EBV陰性的BL(0.504±1.214)和扁桃體組織表達(dá)顯著降低（P㩳0.05），Raji和Ramos比較miR-200a表達(dá)顯著降低（P㩳0.05）。miR-127、miR-155和miR-200a的表達(dá)與c-myc基因比較均無統(tǒng)計(jì)學(xué)意義（P㧐0.05） 結(jié)論： （1）miR-127、miR-155和miR-200a在EBV不同感染狀態(tài)的兒童散發(fā)性BL中表達(dá)存在差異。 （2）miR-127、miR-155的高表達(dá)及miR-200a的低表達(dá)與兒童散發(fā)性BL的發(fā)病機(jī)制及EBV感染密切相關(guān)，可能起著類似癌基因/抑癌基因的作用。
[Abstract]:Objective: To investigate the expression and significance of miR-127 miR-155 and miR-200a in children with Epstein-Barr virus (EBV) infection. Methods: 1. From 2005 to 2012, 40 cases of BL and 15 cases of chronic tonsillitis were collected. The clinicopathological characteristics and follow-up data were analyzed, tissue microarray was prepared, and EBV gene was detected by in situ hybridization. BL was divided into two groups according to the results of EBV gene detection: positive EBV infection group and normal control group. 2. The expression of miR-127, miR-155 and miR-200a in 40 cases of BL and 15 cases of chronic tonsillitis were detected by qRT-PCR method. The expression of miR-127, miR-155 and miR-200a was detected by qRT-PCR method. (4) fluorescence in situ hybridization (fish) technique was used to detect the abnormality of c-myc gene in 40 cases of BL paraffin tissues and BL cell lines. 5. The experimental data were analyzed with 13.0 statistical software package. The data were measured by "mean 鹵standard deviation" and counted by t test. P05 was statistically significant. Results: 1of the 40 cases of BL, 16 cases were positive for EBER, of which 2 cases were located in mesenteric lymph nodes, 4 cases were located in mesenteric lymph nodes, 10 cases were located in ileocecal intestinal tissue, 10 cases were located in ileocecal ileocecum, 24 cases were located in ileocecal ileocecum, and 2 cases were located in 6 cases of mesenteric lymph nodes, among them, 2 cases were located in mesenteric lymph nodes, 6 cases were located in mesenteric lymph nodes, and 2 cases were located in mesenteric lymph nodes. In ileocecal ileocecum, 16 cases were located in ileocecal jejunum (66.67). In mesenteric ileocecal part, 15 cases of tonsil tissue EBER were all negative. 2among 40 cases of BL, 38 cases were positive for c-myc gene breakage. Among them, 23 cases were negative for EBER. 15 cases were positive for c-myc gene signal, and 2 cases were positive for c-myc gene signal. 2 cases were positive for c-myc gene signal, 1 case was positive for EBER gene, and 1 case was positive for c-myc gene breakage in 50%).Raji cell line. 3Compared with EBER negative BL cases and tonsil tissues, miR-127 expression of EBER positive BL cases was up-regulated. The difference was statistically significant. Compared with Ramos cell line and Ramos cell line, miR-127 expression was up-regulated in Ramos cell line. Compared with EBER negative BL cases, the expression of mmiR-155 was up-regulated in EBER positive BL cases, but there was no significant difference between them and tonsil tissues. The difference was statistically significant. In Raji cell line, the expression of miR-155 up-regulated in EBV positive BL was significantly lower than that in EBV negative BL(0.504 鹵1.214) and in tonsils, the expression of miR-200a was significantly lower than that of Ramos and Ramos. There was no significant difference between EBV positive BL and c-myc gene expression. Conclusion: The expression of miR-127 miR-155 and miR-200a in sporadic BL of children with different EBV infection status was different. The high expression of miR-127 miR-155 and the low expression of miR-200a are closely related to the pathogenesis of sporadic BL in children and the infection of EBV, which may play the role of oncogene / tumor suppressor gene.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R733.1

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本文編號(hào)：1966172