本文選題：塵螨變應原 + 肽微陣列��； 參考：《細胞與分子免疫學雜志》2017年08期

【摘要】：目的篩選粉塵螨(Der f)主要和中等效價變應原(Der f1、Der f2、Der f4、Der f5和Der f7)可能具有的線性表位。方法根據(jù)Der f1、Der f2、Der f4、Der f5和Der f7活性部位氨基酸序列,各合成含8個氨基酸的短肽,相互之間重疊7個氨基酸,將這些短肽點到芯片上構建微陣列,將此微陣列芯片與人血清Ig E進行免疫標記反應,掃描芯片并分析數(shù)據(jù)。結果合成了5組變應原共1128個短肽,并構建微陣列芯片。將6份對粉塵螨過敏的兒童血清混合并加樣到微陣列芯片上,進行掃描和數(shù)據(jù)分析,最終得到Der f1的4個表位(第46~53位氨基酸、第71~78位氨基酸、第99~110位氨基酸和第179~186位氨基酸),Der f2的3個表位(第15~22位氨基酸、第80~89位氨基酸和第106~113位氨基酸),Der f4的6個表位(第69~82位氨基酸、第107~116位氨基酸、第225~232位氨基酸、第261~268位氨基酸、第355~365位氨基酸和第483~496位氨基酸),Der f5的1個表位(第102~109位氨基酸)和Der f7的3個表位(第32~39位氨基酸,第52~64位氨基酸和第100~107位氨基酸)。結論確定了粉塵螨變應原Der f1、Der f2、Der f4、Der f5和Der f7線性表位。
[Abstract]:Objective to screen the possible linear epitopes of Der f5 and Der f7 in Der f1, Der f2, Der f4, Der f5 and Der f7, the main and middle titer allergens of Dermatophagoides farinae. Methods according to the amino acid sequences of the active sites of Der f1, Der f2, Der f4, Der f5 and Der f7, each short peptide containing 8 amino acids was synthesized, and 7 amino acids were overlapped with each other. These short peptides were placed on the chip to construct a microarray. The microarray was immunoreactive with human serum IgE, the microarray was scanned and the data were analyzed. Results 1128 short peptides were synthesized from 5 groups of allergens and microarray chips were constructed. Six sera of children allergic to Dermatophagoides farinae were mixed and sampled on microarray chip. The four epitopes of Der F1 were obtained by scanning and data analysis. The three epitopes of the 99th, 110th, 179-, 186th, 225232, 261-, 261-, 89th, 106-, 113amino acids and Der f4 epitopes of Der f4, 69,82,107-, 225232, 261-, 261-, respectively, were found at the 99th, 110th, 186th, 225232, 261-, 261-, 106-, 106-, 113th, 107-, 116th, 225232, 261- and 261268 amino acids, respectively. One epitope of Der f5 (102109amino acid) and three epitopes of Der f7 (32h3t 39-amino acid, 52r-64-amino acid and 100,107amino acid) were found at 355C 365 amino acid and 483H 496 amino acid Der f5. Conclusion the linear epitopes of Der f 2, Der f 4, Der f 5 and Der f 7 of the allergen Der f 1, Der f 2, Der f 4, were identified.
【作者單位】： 江蘇醫(yī)藥職業(yè)學院基礎醫(yī)學部;鹽城市第三人民醫(yī)院檢驗科;
【基金】：國家自然科學基金(31272369)
【分類號】：R725.6
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本文編號：1964971