本文選題：哮喘 + 兒童�。� 參考：《遵義醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:探討調(diào)節(jié)性B細(xì)胞(Regulatory B cells,Breg)在兒童哮喘發(fā)病中可能的作用。方法:選取急性期哮喘患兒23例,平均年齡6.58±2.38歲,男女比例:2.83:1;同年齡健康兒童21例,平均年齡5.43±1.91歲,男女比例2.00:1;(1)采用流式細(xì)胞術(shù)檢測外周血Treg、Th1、Th2、Th17、Breg比例及CD80、CD86、CD1d表達(dá)水平;(2)實(shí)時(shí)熒光定量PCR檢測IFN-γ、T-bet、GATA3、IL-17A、IL-17F、RORγt、IL-10、TGF-βm RNA表達(dá);(3)采用ELISA檢測血清IL-10濃度;結(jié)果:(1)急性期哮喘患兒外周血中CD24hi CD27~+Breg細(xì)胞比例明顯低于正常對照組,共刺激分子CD80、CD86表達(dá)水平低于正常對照組(P0.05),而CD1d的表達(dá)水平有下降趨勢(P0.05);(2)急性期哮喘患兒的CD4~+CD25~+Foxp3~+Treg比例低于正常對照組(P0.05);(3)急性期哮喘患兒外周血清的IL-10濃度低于正常對照組,有統(tǒng)計(jì)學(xué)意義(P0.05);(4)急性期哮喘患兒外周血Th1細(xì)胞比例降低,而Th2及Th17細(xì)胞比例增高,均有統(tǒng)計(jì)學(xué)意義(P0.05);(5)熒光定量PCR檢測觀察到急性期哮喘患兒中CD19~+B細(xì)胞中IL-10的表達(dá)無明顯差異,而TGF-β的表達(dá)水平是升高的有統(tǒng)計(jì)學(xué)意義(P0.05);(6)急性期哮喘患兒CD4~+T細(xì)胞中T-bet、IFNG、RORγt、IL-17A、IL-17F表達(dá)上調(diào),均具有統(tǒng)計(jì)學(xué)意義(P0.05);GATA3表達(dá)未見顯著性差異(P0.05)。結(jié)論:CD24hi CD27~+Breg比例下降及分泌IL-10功能減弱可能是導(dǎo)致急性期哮喘患兒CD4~+T細(xì)胞誘導(dǎo)分化異常的重要因素,共刺激分子CD80、CD86表達(dá)下調(diào)可能也影響了急性期哮喘患兒CD24hiCD27~+Breg的免疫調(diào)節(jié)功能。
[Abstract]:Objective: to investigate the possible role of regulatory B cell Bregin in the pathogenesis of asthma in children. Methods: 23 children with acute asthma (mean age: 6.58 鹵2.38 years) and 21 healthy children of the same age (mean age: 5.43 鹵1.91 years) were selected. Ratio of male to female (2.00: 1 / 1). The ratio of Treg-Th1Th2T / Th17G and CD80 / CD86 / CD1d in peripheral blood were detected by flow cytometry (FCM) and the expression of TGF- 尾 m RNA in peripheral blood was detected by flow cytometry (FCM). Real time quantitative PCR was used to detect the expression of IL-17FOR 緯 IL-10 TGF- 尾 m RNA by real-time quantitative PCR. The serum IL-10 concentration was detected by ELISA. Results the percentage of CD24hi CD27 ~ Breg cells in peripheral blood of children with acute asthma was significantly lower than that of normal controls. The expression level of CD80T CD86 was lower than that of the normal control group (P0.05A) and the expression of CD1d was lower than that of the control group (P0.05-Foxp3- Treg was lower than that of the control group (P0.05FXP3-) the level of IL-10 in the peripheral blood of the children with acute asthma was lower than that of the normal control group (P < 0.05), but the level of IL-10 in the peripheral blood of the children with acute asthma was lower than that of the control group (P < 0.05). The proportion of Th1 cells in peripheral blood of children with acute asthma was decreased, while the proportion of Th2 and Th17 cells was increased. There was no significant difference in the expression of IL-10 in CD19 ~ B cells in children with acute asthma by fluorescence quantitative PCR. The expression of TGF- 尾 in CD4 ~ T cells of children with acute asthma was significantly increased (P 0.05). The expression of TGF- 尾 was up-regulated in CD4 ~ T cells of children with acute asthma. There was no significant difference in the expression of TGF- 尾 between the two groups (P 0.05). Conclusion the decreased proportion of CD24hi CD27 ~ Breg and the decrease of IL-10 secretion may be important factors in inducing abnormal differentiation of CD4 ~ T cells in children with acute asthma. The down-regulation of CD80 and CD86 may also affect the immunomodulatory function of CD24hiCD27 ~ Breg in children with acute asthma.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R725.6

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