本文選題：維生素D + 膿毒癥�。� 參考：《南昌大學(xué)》2017年碩士論文

【摘要】：目的:探討維生素D對(duì)膿毒癥大鼠肺樹突狀細(xì)胞表面分子CD83、CD86表達(dá)水平及抗炎因子IL-10、促炎因子IL-12分泌水平的影響。方法:將雄性(SD)幼年大鼠24只按隨機(jī)數(shù)字表法均分為正常對(duì)照組(Ctrl組),膿毒癥組(LPS組)和維生素D(VitD)干預(yù)組(VitD組)。腹腔注射脂多糖(LPS5mg/kg)[1]制備大鼠膿毒癥模型,VitD組在制備膿毒癥模型之前48h、24h及1h腹腔內(nèi)注射VitD3(1000U/kg)[2]。膿毒癥模型制備后12小時(shí)收集各組大鼠血液及肺組織。用流式細(xì)胞儀檢測肺DCs表面分子CD83和CD86的表達(dá);采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢查肺組織中促炎因子(IL-12)和抗炎因子(IL-10)的含量;采用ELISA法檢查各組大鼠血清25(OH)D水平;肺組織HE染色觀察其病理改變。結(jié)果:幼年大鼠注射LPS后,肺DCs表面分子CD86和CD83的表達(dá)增高,與正常對(duì)照組相比較表達(dá)率明顯上升,差異顯著(P0.05),但VitD組肺DC表面分子CD86和CD83的表達(dá)較LPS組降低,與LPS組比較差異顯著,具有統(tǒng)計(jì)學(xué)意義(P0.05);LPS組肺組織勻漿上清液中IL-12的含量較高,而IL-10的水平較低,以上兩種炎癥因子與正常對(duì)照組相比較均具有統(tǒng)計(jì)學(xué)差異。VitD組肺組織促炎因子IL-12的分泌水平較LPS組降低,而抗炎因子IL-10水平較LPS組顯著升高,且均有統(tǒng)計(jì)學(xué)差異(P0.05);三組中,LPS組血清25(OH)D水平最低,VitD組血清25(OH)D水平較LPS組升高,具有顯著差異(P均0.05),但兩組均明顯低于正常對(duì)照組;肺組織病理檢測顯示LPS組肺組織病理改變顯著,而VitD組肺組織病理改變輕于LPS組。結(jié)論:1、膿毒癥動(dòng)物模型制備成功。2、膿毒癥幼鼠肺組織中DCs表面分子CD83、CD86表達(dá)增加,炎癥因子中促炎因子IL-12分泌增加,IL-10水平降低,提示DCs的活化、炎癥因子的分泌參與了膿毒癥的發(fā)生、發(fā)展。3、VitD可抑制膿毒癥幼鼠肺DCs表面分子CD83、CD86的表達(dá),促進(jìn)抗炎因子IL-10的分泌,抑制促炎因子IL-12的釋放,減輕了機(jī)體過度的炎癥反應(yīng)。
[Abstract]:Aim: to investigate the effects of vitamin D on the expression of CD83- and CD86 on the surface of pulmonary dendritic cells and the secretion of anti-inflammatory cytokines IL-10 and pro-inflammatory factor IL-12 in septic rats. Methods: Twenty-four young male SD rats were randomly divided into normal control group (Ctrl group), septic group (LPS group) and vitamin D Vitamin D (VitD) intervention group (Vit D group). Lipopolysaccharide (LPS 5 mg / kg) [1] the sepsis model of rats was induced by intraperitoneal injection of VitD (1000Ukg) at 48h, 24h and 1h before the model of sepsis was established. Blood and lung tissues were collected 12 hours after sepsis model. The expression of CD83 and CD86 on lung DCs was detected by flow cytometry, the levels of proinflammatory factor IL-12 (IL-12) and anti-inflammatory factor (IL-10) in lung tissue were detected by Elisa, and the serum 25(OH)D level of rats was detected by ELISA method. The pathological changes of lung tissue were observed by HE staining. Results: after injection of LPS, the expression of CD86 and CD83 on lung DCs surface in young rats was increased, and the expression rate of CD86 and CD83 was significantly higher than that of normal control group (P 0.05). However, the expression of CD86 and CD83 on lung DC in VitD group was lower than that in LPS group. Compared with LPS group, the content of IL-12 in lung homogenate supernatant was higher, but the level of IL-10 was lower. The level of IL-12 in lung tissue in Vit D group was lower than that in LPS group, while the IL-10 level of anti-inflammatory factor in Vit D group was significantly higher than that in LPS group. The level of serum 25(OH)D in 25(OH)D group was significantly higher than that in LPS group (P 0.05), but it was significantly lower in both groups than that in normal control group. The pathological changes of lung tissue in LPS group were significantly higher than that in LPS group, while that in VitD group was less than that in LPS group. Conclusion: 1, the animal model of sepsis was successfully established. The expression of CD83 CD86 on the surface of DCs and the secretion of proinflammatory factor IL-12 in inflammatory factors were increased and the level of IL-10 was decreased in the lung tissue of septic young rats, indicating the activation of DCs. The secretion of inflammatory factors is involved in the occurrence of sepsis. The development of Vit D can inhibit the expression of CD83 CD86 on the surface of pulmonary DCs, promote the secretion of anti inflammatory factor IL-10, inhibit the release of proinflammatory factor IL-12, and alleviate the excessive inflammatory reaction of the body.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R720.597

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 孫樂科;吳儉;;維生素D對(duì)膿毒癥小鼠炎癥的抑制作用[J];江西醫(yī)藥;2017年01期

2 賈艷麗;王英;程浩;孫懿;;維持性血液透析患者血清Klotho蛋白水平及其影響因素[J];臨床薈萃;2015年08期

3 陽琰;高琳;李顯文;張晗;廖鑫;陳其榮;宋美oS;;阿法骨化醇對(duì)新診斷2型糖尿病患者胰島β細(xì)胞第一時(shí)相分泌的影響[J];遵義醫(yī)學(xué)院學(xué)報(bào);2015年02期

4 張瑩瑩;鄭健斌;;血清25-羥基維生素D水平與PICU患兒預(yù)后的關(guān)系[J];廣東醫(yī)學(xué);2014年13期

5 靳文;李志j;傅強(qiáng);;氧化低密度脂蛋白對(duì)樹突狀細(xì)胞表面CD36表達(dá)的影響[J];現(xiàn)代醫(yī)院;2013年08期

6 劉軍;張朋書;于晴;邱海波;;一種高效的小鼠肺單細(xì)胞懸液制備方法的研究[J];東南大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2012年02期

7 趙亞娟;富建華;孫梅;;谷氨酰胺影響膿毒癥大鼠腦內(nèi)PDGF及其受體表達(dá)的研究[J];中國當(dāng)代兒科雜志;2010年12期

8 劉娟;錢素云;;小兒膿毒癥和嚴(yán)重膿毒癥發(fā)病情況單中心調(diào)查[J];臨床兒科雜志;2010年01期

9 田光;陸江陽;胡森;呂藝;王宏偉;楊毅;;卡巴膽堿對(duì)膿毒癥小鼠脾臟樹突狀細(xì)胞變化的影響[J];中國危重病急救醫(yī)學(xué);2006年11期

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