發(fā)布時間：2018-04-26 02:19

  本文選題：星狀病毒 + 腹瀉��； 參考：《環(huán)境與健康雜志》2017年06期

【摘要】：目的了解天津市秋冬季腹瀉患兒的星狀病毒感染情況及其分型特點。方法采用逆轉錄聚合酶鏈反應(RTPCR)技術,于2014年9月—2015年2月收集天津市兒童醫(yī)院住院部1 127例疑似病毒性腹瀉的嬰幼兒糞便樣品中的星狀病毒核酸進行檢測,并將部分陽性株的PCR產物進行測序,采用序列分析軟件分析測序結果,推斷其毒株型別,同時收集患兒的臨床資料進行綜合分析。結果 1 127份腹瀉糞便樣品中,星狀病毒RT-PCR陽性99份,檢出率為8.78%。感染星狀病毒的患兒多為1歲以下,患兒年齡中位數(shù)為3.00月齡,且1月檢出率最高。99例陽性星狀病毒患兒中,有52例與其他病毒混合感染,混合感染率為4.61%,其中以諾如病毒合并星狀病毒最常見。26份PCR陽性產物送往測序,經Genbank Blast及系統(tǒng)進化樹分析,所有星狀病毒均為人類星狀病毒1型。結論星狀病毒是天津市秋冬季嬰幼兒病毒性腹瀉的重要病原體,本次調查期間星狀病毒以人類星狀病毒1型毒株流行為主,此外存在一定比例的混合感染。
[Abstract]:Objective to investigate the status of stellate virus infection and its typing in children with diarrhea in autumn and winter in Tianjin. Methods from September 2014 to February 2015, stellate virus nucleic acids were collected from 1,127 samples of infant faeces suspected of viral diarrhea in Tianjin Children's Hospital from September 2014 to February 2015 by reverse transcriptase polymerase chain reaction (RT-PCR). The PCR products of some positive strains were sequenced. The results of sequencing were analyzed by sequence analysis software, and the type of virus strains was inferred, and the clinical data of children were collected for comprehensive analysis. Results among 1 127 fecal samples of diarrhea, 99 were stellate virus RT-PCR positive, the detection rate was 8.78. Most of the children infected with stellate virus were under 1 year old, and the median age was 3.00 months old. The highest detection rate of stellate virus was found in January, 52 of them were mixed with other viruses. The mixed infection rate was 4.61%. The most common PCR positive products of Enoviruses combined with stellate virus were sent to sequencing. By Genbank Blast and phylogenetic tree analysis, all stellate viruses were human stellate virus type 1. Conclusion stellate virus is an important pathogen of infantile viral diarrhea in autumn and winter in Tianjin. During this investigation, human stellate virus type 1 was the predominant virus strain, and there was a certain proportion of mixed infection.
【作者單位】： 天津市兒童醫(yī)院檢驗科;軍事醫(yī)學科學院衛(wèi)生學環(huán)境醫(yī)學研究所;
【基金】：天津市科技支撐計劃項目(16YFZCSF00340)
【分類號】：R446.5;R725.1

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