本文選題：小兒先心病 + 肺動(dòng)脈高壓　； 參考：《北京協(xié)和醫(yī)學(xué)院》2016年碩士論文

【摘要】：目的：通過(guò)探索小兒先心病肺動(dòng)脈高壓(pulmonary arterial hypertension,PAH)患者血清腦利尿肽(brain natriuretic factor or peptide,BNP)的濃度和microRNA (miRNA)的表達(dá)水平,研究它們與PAH的關(guān)系及彼此之間的相關(guān)性,從而為先心病肺動(dòng)脈高壓的診斷提供幫助。方法：收集泰達(dá)國(guó)際心血管病醫(yī)院2015年10月至2016年4月收治確診為先天性心臟病患者39例。其中男性21例,女性18例。根據(jù)2008年肺動(dòng)脈高壓國(guó)際論壇會(huì)公布的肺動(dòng)脈高壓的診斷標(biāo)準(zhǔn)進(jìn)行分組,分別為先天性心臟病肺動(dòng)脈壓力正常組16例(A組)；先天性心臟病合并輕、中度肺動(dòng)脈高壓組(B組)15例和先天性心臟病重度動(dòng)脈高壓組(c組)8例。采集受試者外周靜脈血,利用BNP免疫反應(yīng)試劑盒對(duì)血清BNP濃度進(jìn)行檢測(cè)；同時(shí)利用實(shí)時(shí)定量PCR對(duì)miRNA表達(dá)量進(jìn)行測(cè)定。利用SPSS軟件對(duì)數(shù)據(jù)進(jìn)行ANOVA分析,并做ROC曲線,同時(shí)利用Person相關(guān)性分析BNP與miRNA共同診斷PAH的合同效果。結(jié)果：(1)對(duì)受試者血清BNP濃度進(jìn)行檢測(cè),結(jié)果顯示,先心病肺高壓重度患者血清BNP濃度為183.35±6.36,明顯高于有先心病但肺動(dòng)脈血壓正常組(p0.01)。(2)肺動(dòng)脈重度患者miRNA19a表達(dá)量為3.28±0.26,顯著高于A、B組。ROC曲線下方面積為0.906.(3) BNP與miRNA-19a之間的相關(guān)性為0.778,具有統(tǒng)計(jì)學(xué)差異。結(jié)論：1小兒先心病肺動(dòng)脈高壓患者血清BNP濃度可以作為臨床診斷指標(biāo)2.miRNA-19a在先心病肺動(dòng)脈早期表達(dá)量都有所增加,可作為早期預(yù)警指標(biāo)。3.BNP濃度和miRNA-19a的相關(guān)性較高,可以協(xié)同作用,提高確診率。
[Abstract]:Objective: to investigate the serum concentration of brain natriuretic factor or microRNA miRNAs in children with pulmonary arterial hypertensionation of congenital heart disease, and to study the relationship between PAH and serum brain natriuretic factor or microRNA miRNAs. It is helpful for the diagnosis of pulmonary hypertension of congenital heart disease. Methods: 39 patients with congenital heart disease were collected from October 2015 to April 2016 in Teda International Cardiovascular Hospital. There were 21 males and 18 females. According to the diagnostic criteria of pulmonary hypertension published by the International Forum on Pulmonary Hypertension in 2008, 16 patients with congenital heart disease with normal pulmonary artery pressure were divided into two groups: group A, congenital heart disease complicated with mild heart disease, 15 cases in group B of moderate pulmonary hypertension and 8 cases in group C of severe congenital heart disease. The peripheral venous blood was collected and the serum BNP concentration was detected by BNP immunoassay kit, and the expression of miRNA was measured by real-time quantitative PCR. The SPSS software is used to analyze the data by ANOVA, and the ROC curve is made. At the same time, the Person correlation analysis is used to analyze the contract effect of BNP and miRNA in the diagnosis of PAH. Results the serum BNP concentration of the subjects was measured and the results showed that, The level of serum BNP in patients with severe pulmonary hypertension was 183.35 鹵6.36, which was significantly higher than that in patients with CHD but normal pulmonary blood pressure (P 0.01). The expression of miRNA19a in patients with severe pulmonary artery was 3.28 鹵0.26, which was significantly higher than that in group A B, where the area under the curve was 0.906.63) between BNP and miRNA-19a. The correlation was 0.778, with statistical difference. Conclusion the serum BNP concentration in children with congenital heart disease and pulmonary hypertension can be used as a clinical diagnostic index to increase the expression of 2.miRNA-19a in the early stage of pulmonary artery disease, and it can be used as an early warning index. 3. The correlation between the concentration of BNP and miRNA-19a is relatively high, and it can be used as a synergistic index. Increase the rate of diagnosis.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R725.4

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃震華;肺動(dòng)脈高壓治療進(jìn)展[J];高原醫(yī)學(xué)雜志;2001年01期

2 袁志明;阻塞性睡眠呼吸暫停綜合征與肺動(dòng)脈高壓研究進(jìn)展[J];國(guó)外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);2002年03期

3 郭明好,牛文革,劉云,劉向東,許清玉,楊磊;激素加環(huán)磷酰胺沖擊治療原發(fā)性干燥綜合征合并肺動(dòng)脈高壓5例[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2003年14期

4 王虹,王祥;肺源性肺動(dòng)脈高壓研究進(jìn)展[J];江蘇醫(yī)藥;2004年09期

5 朱萍,倪松石;慢性阻塞性肺疾病并發(fā)肺動(dòng)脈高壓的治療進(jìn)展[J];中國(guó)交通醫(yī)學(xué)雜志;2004年04期

6 鄧長(zhǎng)金;混合性結(jié)締組織病致肺動(dòng)脈高壓1例[J];臨床心血管病雜志;2005年04期

7 錢衛(wèi)民;珠蛋白生成障礙性貧血合并肺動(dòng)脈高壓1例[J];嶺南心血管病雜志;2005年05期

8 彭翠萍;;混合性結(jié)締組織病合并肺動(dòng)脈高壓1例[J];罕少疾病雜志;2005年06期

9 劉超,劉剛,劉坤申;內(nèi)皮素受體拮抗劑與肺動(dòng)脈高壓的治療[J];中國(guó)實(shí)用內(nèi)科雜志;2005年01期

10 陸慰萱;;肺動(dòng)脈高壓的新分類[J];中國(guó)實(shí)用內(nèi)科雜志;2006年01期

相關(guān)會(huì)議論文 前10條

1 沈敏;張p，

本文編號(hào)：1785622