本文選題：雷公藤多苷 + 青春期大鼠��； 參考：《寧夏醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的觀察雷公藤多苷對(duì)青春期大鼠睪丸組織結(jié)構(gòu)及精原細(xì)胞分化、血清睪酮的影響并探討其機(jī)制。方法青春期雄性SD大鼠（postnatal day35，PND35）48只，隨機(jī)分為3組（n=16），對(duì)照組給予1%羧甲基纖維素鈉6ml/（kg d）、雷公藤多苷單倍劑量組給予雷公藤多苷6mg/（kg d）、雙倍劑量組給予12mg/（kg d）灌胃至性成熟早期（PND63）。停藥24小時(shí)每組隨機(jī)取8只，檢測睪丸重量、睪丸臟器系數(shù)、生精細(xì)胞計(jì)數(shù)，光鏡、電鏡下觀察睪丸組織學(xué)改變；用免疫組化法檢測生精細(xì)胞c-kit表達(dá)；酶聯(lián)免疫吸附試驗(yàn)測定血清睪酮濃度。剩余大鼠于停藥后4周（PND92）檢測上述各指標(biāo)。結(jié)果停藥24小時(shí)，與對(duì)照組比較，兩個(gè)實(shí)驗(yàn)組睪丸重量、睪丸臟器系數(shù)及血清睪酮含量均無明顯變化（P0.05），支持細(xì)胞及間質(zhì)組織較對(duì)照組無明顯變化；單倍劑量組生精細(xì)胞計(jì)數(shù)減少、c-kit表達(dá)下調(diào)（P0.05）；雙倍劑量組生精細(xì)胞計(jì)數(shù)較對(duì)照組明顯減少，c-kit表達(dá)下調(diào)（P0.05）。停藥4周，單倍劑量組睪丸重量、臟器系數(shù)、生精細(xì)胞計(jì)數(shù)、生精細(xì)胞c-kit表達(dá)及血清睪酮含量與對(duì)照組比較差別無統(tǒng)計(jì)學(xué)意義（P0.05），電鏡下可見支持細(xì)胞萎縮；雙倍劑量組睪丸重量、生精細(xì)胞計(jì)數(shù)明顯下降（P0.01）、臟器系數(shù)明顯降低（P0.05）、生精細(xì)胞c-kit表達(dá)下調(diào)、血清睪酮水平明顯降低(P0.01)，生精上皮僅可見少量精原細(xì)胞及支持細(xì)胞，支持細(xì)胞空泡樣變，緊密連接分離，曲細(xì)精管萎縮，基膜迂曲、斷裂，間質(zhì)水腫，間質(zhì)血管內(nèi)皮細(xì)胞增生，血管基膜斷裂，血管腔狹窄。結(jié)論1、雷公藤多苷可致青春期大鼠睪丸組織損傷，，損傷程度與劑量有關(guān)，雙倍劑量所致?lián)p傷較單倍劑量明顯，并可引起血清睪酮含量降低；單倍劑量雷公藤多苷所致生精細(xì)胞損傷停藥后有所恢復(fù)；2、雷公藤多苷對(duì)青春期大鼠睪丸組織的損傷有遲發(fā)性；3、雷公藤多苷所致青春期大鼠睪丸組織遲發(fā)性損傷的機(jī)制包括抑制精原細(xì)胞分化、破壞血睪屏障以及影響睪丸組織血供，這些機(jī)制可能是雷公藤多苷導(dǎo)致青春期睪丸組織損傷的重要原因。
[Abstract]:Objective to investigate the effects of tripterygium wilfordii polyglycosides on testicular structure, spermatogonial differentiation and serum testosterone in adolescent rats.Methods Forty-eight male Sprague-Dawley rats were randomly divided into 3 groups. The control group was given 1% carboxymethyl cellulose sodium 6ml/(kg dronium, the tripterygium wilfordii polyglycoside 6mg/(kg dai group was given tripterygium wilfordii polyglycoside 6mg/(kg dung, and the double dose group was given 12mg/(kg d) until the early stage of sexual maturation.The weight of testis, testicular organ coefficient, spermatogenic cell count, histological changes of testis were observed under light microscope and electron microscope, and the expression of c-kit in spermatogenic cells was detected by immunohistochemical method.Serum testosterone concentration was determined by enzyme linked immunosorbent assay (Elisa).All the above indexes were detected by PND 92 in the remaining rats 4 weeks after withdrawal.Results there were no significant changes in testicular weight, testicular organ coefficient and serum testosterone content in the two experimental groups compared with the control group for 24 hours. The Sertoli cells and interstitial tissue had no significant change compared with the control group.The number of spermatogenic cells in the single dose group decreased significantly compared with that in the control group, and that in the double dose group was significantly lower than that in the control group.After 4 weeks of withdrawal, testicular weight, organ coefficient, spermatogenic cell count, c-kit expression and serum testosterone content of spermatogenic cells in the single dose group were not significantly different from those in the control group (P 0.05). Atrophy of Sertoli cells was observed under electron microscope.In double dose group, testicular weight, spermatogenic cell count and viscera coefficient decreased significantly, c-kit expression in spermatogenic cells decreased, serum testosterone level decreased significantly, only a few spermatogonial cells and Sertoli cells were found in spermatogenic epithelium.Vacuolar degeneration of Sertoli cells, tight junction separation, atrophy of seminiferous tubules, convoluted basal membrane, rupture of basement membrane, interstitial edema, proliferation of vascular endothelial cells, rupture of basement membrane and stenosis of blood lumen.Conclusion (1) Tripterygium wilfordii polyglycosides can cause testicular tissue injury in puberty rats, the degree of injury is related to the dose, the damage caused by double dose is more obvious than that by single dose, and the serum testosterone content can be decreased by tripterygium wilfordii.The injury of spermatogenic cells induced by tripterygium wilfordii polyglycosides at a single dose recovered 2%. The damage of tripterygium wilfordii polyglycosides to testis of pubertal rats was delayed, and that of tripterygium wilfordii polyglycosides was delayed injury in testis of pubertal rats.Mechanisms include inhibition of spermatogonia differentiation,The destruction of the blood-testis barrier and the influence of the blood supply of testis may be the important causes of the injury of testicular tissue caused by tripterygium wilfordii polyglycosides.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R285.5;R726.9

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