本文選題：宮內(nèi)發(fā)育遲緩 + 生長相關(guān)蛋白-43　； 參考：《昆明醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：本研究通過孕期全程低蛋白飲食法建立宮內(nèi)發(fā)育遲緩(intrauterine growth retardation, IUGR)的實驗動物模型,觀察生長相關(guān)蛋白-43(growth associated protein-43,GAP-43)在IUGR大鼠腦組織中不同部位及不同發(fā)育階段的表達變化情況,分析GAP-43在IUGR大鼠發(fā)生腦發(fā)育及功能異常中的作用機制,為臨床干預(yù)及治療提供相關(guān)理論依據(jù)。 方法：①采用孕期全程低蛋白飲食法建立IUGR實驗動物模型。②母鼠按受孕順序隨機分為低蛋白飲食組和正常飲食組。低蛋白飲食組中,符合IUGR診斷標準的仔鼠按隨機原則抽取30只作為IUGR組；正常飲食組中,正常出生體重仔鼠按隨機原則抽取30只作為對照組；分別將兩組實驗仔鼠按隨機原則分為出生后0天、10天、30天三個亞組。③兩組實驗仔鼠分別在出生后0天、10天、30天計量體重及腦重。④用HE染色法觀察兩組實驗仔鼠在不同發(fā)育階段腦組織的病理特征。⑤用免疫組化法觀察兩組實驗仔鼠在不同生長發(fā)育階段腦皮質(zhì)內(nèi)及海馬CA1區(qū)、CA3區(qū)GAP-43蛋白的表達改變情況。⑥熒光定量聚合酶鏈反應(yīng)(fluorescence quantitative polymerase chain reaction,FQ-PCR)法檢測兩組實驗仔鼠在不同生長發(fā)育階段大腦海馬區(qū)GAP-43mRNA的表達改變情況。 結(jié)果：①低蛋白飲食組IUGR發(fā)生率(62.5%)顯著高于正常飲食組(7.89%,P0.05)。②低蛋白飲食組死胎率(17.94%)顯著高于正常飲食組(5.0%,P0.05)。③IUGR組仔鼠出生后0天體重(4.6847±0.3126g)顯著低于對照組(6.4352±0.3135g,P0.01)；出生后10天、30天兩組體重?zé)o明顯差異(P0.05)。④兩組仔鼠腦重在出生后0天、10天、30天時,IUGR組均明顯低于對照組,差異有統(tǒng)計學(xué)意義(P0.01)。⑤GAP-43蛋白在仔鼠大腦皮質(zhì)區(qū)的表達,IUGR組出生后0天、10天均明顯低于對照組(P0.01)；出生后30天無明顯差異(P0.05)。⑥GAP-43蛋白在仔鼠海馬CA1區(qū)、CA3區(qū)的表達,出生后10天IUGR組明顯低于對照組(P0.01),出生后0天、30天無明顯差異(P0.05)。⑦FQ-PCR相對定量結(jié)果示兩組仔鼠出生0天時海馬區(qū)GAP-43mRNA的表達水平無明顯差異(P0.05)；出生后10天IUGR組明顯低于對照組,差異有統(tǒng)計學(xué)意義(P0.01),出生后30天無明顯差異(P0.05)。 結(jié)論： 1.孕期全程低蛋白飲食法為較理想的IUGR造模方法。 2. IUGR組仔鼠腦質(zhì)量的發(fā)育在較長時期內(nèi)低于對照組。 3. IUGR組仔鼠出生時即存在腦皮質(zhì)發(fā)育異常,并持續(xù)至出生后30天(約人類8歲)。GAP-43在海馬區(qū)的低表達可能與IUGR兒發(fā)生遠期學(xué)習(xí)、記憶等智能發(fā)育障礙有關(guān)。
[Abstract]:Objective: To study the method of whole pregnancy low protein diet to establish intrauterine growth retardation (intrauterine growth, retardation, IUGR) of the experimental animal model, to observe the growth associated protein -43 (growth associated protein-43, GAP-43) in the brain tissue of IUGR rats and the expression of different parts in different developmental stages, GAP-43 analysis of mechanism of brain development and abnormal function in IUGR rats, to provide theoretical basis for clinical treatment and intervention.
Method: to establish the animal model of IUGR by the method of whole pregnancy low protein diet. The rats in pregnancy were randomly divided into the low protein diet group and normal diet group. The low protein diet group, according to the diagnosis criteria of IUGR rats were randomly selected 30 rats as IUGR group; normal diet group, normal birth weight rats were randomly selected 30 rats as control group; the two groups of experimental rats were randomly divided into 0 days, 10 days after birth, 30 days and three sub groups. The two groups of experimental rats were born after 0 days, 10 days, 30 days to measure the weight of body and brain. Using HE staining to observe the pathological features of two groups of experimental rats at different developmental stages of brain tissue. The immunohistochemistry was used to observe two groups of experimental rats at different developmental stages in the cerebral cortex and hippocampal CA1 region, the expression of CA3 GAP-43 protein. The changes of fluorescence quantitative polymerase chain Fluorescence (quantitative polymerase chain reaction, FQ-PCR) method was used to detect the expression of GAP-43mRNA in the hippocampus of two groups of experimental offspring at different growth stages.
緇撴灉錛氣憼浣庤泲鐧介ギ椋熺粍IUGR鍙戠敓鐜，

本文編號：1733699