本文選題：肺損傷　切入點(diǎn)：血紅素加氧酶-　出處：《重慶醫(yī)學(xué)》2017年26期

【摘要】：目的探討血紅素加氧酶-1(HO-1)在高氧造成的肺損傷轉(zhuǎn)基因小鼠和正常小鼠中的肺部表達(dá)水平及其作用。方法把32只新生小鼠分成4組:野生型組(WT組),特異性轉(zhuǎn)基因小鼠全身高水平表達(dá)HO-1組[HO-1-FL(H)組,細(xì)胞質(zhì)]、特異性轉(zhuǎn)基因小鼠全身低水平表達(dá)HO-1組[HO-1-FL(L)組,細(xì)胞質(zhì)]和切去C末端HO-1(Nuc-HO-1-TR組,細(xì)胞核)。把4組小鼠暴露在高氧環(huán)境中3d,然后放到正�？諝猸h(huán)境中,通過免疫組織化學(xué)、免疫熒光等實(shí)驗(yàn)技術(shù)來觀察小鼠3d、7d和14d肺泡發(fā)育情況及HO-1在小鼠肺部表達(dá)情況。結(jié)果 HO-1在HO-1-FL(H)組小鼠肺部高表達(dá),高氧暴露3d后4組小鼠肺部肺泡發(fā)育都受到了損害,在正�？諝猸h(huán)境中7、14d時(shí)HO-1-FL(H)組小鼠的肺泡發(fā)育恢復(fù)得最好。結(jié)論小鼠肺部適度高水平的HO-1表達(dá)有助于高氧環(huán)境造成的小鼠肺損傷的恢復(fù)。
[Abstract]:Objective to investigate the lung expression of heme oxygenase-1 (HO-1) in hyperoxia-induced lung injury transgenic mice and normal mice. Methods 32 newborn mice were divided into 4 groups: wild type group, WT group, and specific transgenic group. High level expression of HO-1 group [HO-1-FLH] group, Specific transgenic mice expressed low level of HO-1 group [HO-1-FLL group, cytoplasm] and C terminal HO-1(Nuc-HO-1-TR group, nucleus. 4 groups of mice were exposed to hyperoxic environment for 3 days, then put into normal air environment, through immunohistochemistry, 4 groups of mice were exposed to hyperoxia for 3 days. Immunofluorescence technique was used to observe the alveolar development and the expression of HO-1 in the lungs of mice on day 7 and 14. Results the expression of HO-1 in the lungs of HO-1-FLH) group was high, and the alveolar development of the four groups were damaged after 3 days of hyperoxia exposure. The alveolar development of HO-1-FLH) group was the best in normal air environment for 14 days. Conclusion the moderate and high level of HO-1 expression in the lung of mice is helpful to the recovery of lung injury induced by hyperoxia.
【作者單位】： 錦州醫(yī)科大學(xué)附屬第三醫(yī)院檢驗(yàn)中心;錦州醫(yī)科大學(xué)附屬第一醫(yī)院胸外科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81541099)
【分類號(hào)】：R722.1

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