本文選題：早產(chǎn)兒　切入點：高氧　出處：《中國現(xiàn)代醫(yī)學雜志》2017年09期

【摘要】：目的探討高氧暴露早產(chǎn)兒外周血單個核細胞應(yīng)用白藜蘆醇對氧化應(yīng)激的影響。方法 52例早產(chǎn)兒隨機分為白藜蘆醇+高氧組和高氧組,每組26例,選取同期吸入常壓空氣早產(chǎn)兒20例作為對照組。抽取患兒橈動脈血2 ml,采用Ficoll密度梯度離心法分離單個核細胞。觀察3組單個核細胞活性氧水平、培養(yǎng)液丙二醛(MDA)水平、細胞自噬體、血紅細胞加氧酶(HO-1)蛋白水平及活性。結(jié)果 3組活性氧、MDA、細胞自噬體、HO-1蛋白水平、HO-1活性比較,差異有統(tǒng)計學意義(P0.05),高氧組活性氧、MDA、細胞自噬體、HO-1蛋白水平、HO-1活性高于對照組和白藜蘆醇+高氧組;白藜蘆醇+高氧組活性氧、MDA、細胞自噬體、HO-1蛋白水平、HO-1活性高于對照組。結(jié)論白藜蘆醇能夠有效對抗高氧暴露產(chǎn)生的氧化應(yīng)激,減少氧化應(yīng)激損傷。
[Abstract]:Objective to investigate the effect of resveratrol on oxidative stress in peripheral blood mononuclear cells of premature infants exposed to hyperoxia. Methods 52 premature infants were randomly divided into two groups: resveratrol hyperoxia group and hyperoxia group. Twenty premature infants with atmospheric pressure were selected as control group. The blood of radial artery was extracted from the control group. Mononuclear cells were isolated by Ficoll density gradient centrifugation. The levels of reactive oxygen species (Ros) in mononuclear cells and malondialdehyde (MDA) in culture medium were observed in three groups. Results the level and activity of HO-1 protein were compared among the three groups of reactive oxygen species (Ros) and HO-1 (HO-1). The difference was statistically significant (P 0.05). The activity of HO-1 and the level of HO-1 protein in macrophages in hyperoxia group were higher than those in control group and resveratrol hyperoxia group. In resveratrol hyperoxia group, the activity of HO-1 protein in macrophages was higher than that in control group. Conclusion resveratrol can effectively antagonize oxidative stress induced by hyperoxia exposure and reduce oxidative stress injury.
【作者單位】： 南陽醫(yī)學高等�？茖W校第一附屬醫(yī)院兒科;
【基金】：河南省教育廳科學技術(shù)研究重點項目(No:142102810950)
【分類號】：R722.6

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