發(fā)布時(shí)間：2018-03-06 15:41

  本文選題：病毒性心肌炎　切入點(diǎn)：骨髓間充質(zhì)干細(xì)胞　出處：《臨床兒科雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的探討骨髓間充質(zhì)干細(xì)胞(BMSCs)對小鼠病毒性肌炎的作用。方法 4周齡BALB/C雄性小鼠隨機(jī)分為,正常對照組、心肌炎組、心肌炎不同階段(3天、2周)BMSCs干預(yù)組,腹腔注射柯薩奇病毒B3建立病毒性心肌炎小鼠模型,干預(yù)組分別于注射病毒后3天、2周尾靜脈注射小鼠BMSCs,4周后行心臟超聲檢查,光鏡觀察并計(jì)算心肌組織病理積分及膠原容積分?jǐn)?shù)(CVF),q RT-PCR法檢測心肌組織勻漿膠原纖維I(col1α1)和膠原纖維III(col3α1)m RNA表達(dá)。結(jié)果與正常對照組相比,心肌炎組左室前后壁變薄,左室收縮期末期內(nèi)徑和容積增大,左室射血分?jǐn)?shù)(LVEF)及短軸縮短率(FS)降低,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05);干預(yù)組各亞組LVEF及FS較心肌炎組均有改善,其中心肌炎2周時(shí)干預(yù)組改善更明顯,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05)。光鏡下,心肌炎組心肌組織CVF較正常對照組增高,干預(yù)組CVF較心肌炎組降低,其中心肌炎2周干預(yù)組CVF較心肌炎3天干預(yù)組更低,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05)。與正常對照組相比,心肌炎組col1α1和col3α1 m RNA表達(dá)均升高,干預(yù)組col1α1和col3α1 m RNA表達(dá)較心肌炎組降低,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05)。結(jié)論 BMSCs可減少病毒性肌炎小鼠心臟纖維化程度及改善心功能,且病毒感染2周時(shí)干預(yù)效果更佳。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on viral myositis in mice. Methods BALB/C male mice aged 4 weeks were randomly divided into normal control group, myocarditis group and 2 weeks intervention group. The mice model of viral myocarditis was established by intraperitoneal injection of Coxsackie virus B3. The intervention group received echocardiography 4 weeks after the injection of BMSCs5 into mice at the end of 2 weeks after the injection of the virus. Myocardial histopathological score and collagen volume fraction (CVFV / Q RT-PCR) were observed and calculated under light microscope to detect the expression of collagen fiber Ifcol1 偽 1) and collagen fiber III(col3 偽 1 m RNA in myocardial homogenate. Results compared with the control group, the left ventricular wall in myocarditis group was thinner than that in the control group, and the anterior and posterior wall of the left ventricle in the myocarditis group was thinner than that in the control group. Left ventricular end-systolic diameter and volume increased, left ventricular ejection fraction (LVEF) and short axis shortening rate decreased (P < 0.05), LVEF and FS in intervention group were improved as compared with those in myocarditis group. The myocardial CVF in the myocarditis group was higher than that in the normal control group under light microscope, and the CVF in the intervention group was lower than that in the myocarditis group. The expression of col1 偽 1 and col3 偽 1 m RNA in myocarditis group was higher than that in myocarditis group at 2 weeks, and the expression of col1 偽 1 and col3 偽 1 m RNA in intervention group was lower than that in myocarditis group. Conclusion BMSCs can reduce the degree of cardiac fibrosis and improve cardiac function in mice with viral myositis, and the intervention effect is better at 2 weeks after viral infection.
【作者單位】： 溫州醫(yī)科大學(xué)附屬第二醫(yī)院育英兒童醫(yī)院;
【基金】：浙江省科技廳公益技術(shù)研究社會(huì)發(fā)展項(xiàng)目(No.2014C33164)
【分類號(hào)】：R725.4

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