本文選題：SP-A2　切入點(diǎn)：單體型　出處：《廣西醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本研究主要目的為了探討肺表面活性蛋白A2(SP-A2)單體型與早產(chǎn)兒呼吸窘迫綜合征(RDS)發(fā)病的關(guān)系,以及了解SP-A2單體型在廣西局部地區(qū)人群中的分布情況。方法：研究采用病例對(duì)照研究和人群研究的方法,選取80例患有RDS的早產(chǎn)兒及80例非RDS早產(chǎn)兒,同時(shí)選取足月分娩(包含順產(chǎn)及剖宮產(chǎn))的80例健康嬰兒作為研究對(duì)象；采集他們的靜脈或臍帶抗凝血,使用DNA提取試劑盒提取基因組DNA；應(yīng)用TaqMan(?)實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)(polymerase chain reaction, PCR)方法對(duì)SP-A2基因上的4個(gè)單核甘酸多態(tài)性位點(diǎn)(single nucleotide polymorphism, SNP) (rs1059046、rs17886395、 rs1965707、rs1965708)進(jìn)行基因分型；每個(gè)SNP位點(diǎn)隨機(jī)抽取45例標(biāo)本應(yīng)用聚合酶鏈?zhǔn)椒磻?yīng)(polymerase chain reaction, PCR)和DNA測(cè)序技術(shù)驗(yàn)證TaqMan(?)實(shí)時(shí)熒光定量PCR的基因分型結(jié)果。結(jié)果：1、病例組和對(duì)照組的一般情況：所有研究對(duì)象在性別、胎齡、體重、雙胎妊娠及出生方式等情況在病例組和對(duì)照組比較中差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。2、SP-A2基因上的4個(gè)位點(diǎn)(rs1059046、rs17886395、rs1965707、 rs1965708)在足月兒組中的基因型頻率分布符合哈迪-溫伯格平衡(P0.05)。足月兒主要表達(dá)的單體型為1A0、1A5、1A1,頻率分別為54.2%、21%、 9.7%。3、SP-A2各單體型頻率分布在早產(chǎn)兒病例組和對(duì)照組總?cè)巳罕容^中差異均無統(tǒng)計(jì)學(xué)差異(P0.05)。4、以胎齡32周為界,進(jìn)行分組后(胎齡≥32周及32周),再分別對(duì)病例組和對(duì)照組進(jìn)行比較,發(fā)現(xiàn)SP-A2各單體型頻率分布在兩組中比較差異仍無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論：1、未發(fā)現(xiàn)SP-A2的各單體型與早產(chǎn)兒RDS發(fā)病存在關(guān)聯(lián)。2、在廣西局部漢族人群中SP-A2的單體型分布有一定特異性。
[Abstract]:Objective: to investigate the relationship between pulmonary surfactant protein A2SP-A2 haplotype and respiratory distress syndrome (RDS) in premature infants. To understand the distribution of SP-A2 haplotype in local population in Guangxi. Methods: 80 premature infants with RDS and 80 non RDS premature infants were selected by case-control study and population study. At the same time, 80 healthy infants in term delivery (including normal delivery and caesarean section) were selected as study objects; their vein or umbilical cord anticoagulant blood was collected, DNA extraction kit was used to extract genomic DNA, and Taq Mann was used to extract genomic DNA. ) the single chain polymorphisms (rs1059046, rs178863955, rs1965707rs1965708) of four mononuclear glycosylated polymorphic loci on SP-A2 gene were genotyped by real-time fluorescence quantitative polymerase chain reaction (PCR). Polymerase chain reaction (PCR) and DNA sequencing were used to verify Taq Mann? Results of real-time fluorescent quantitative PCR genotyping. Results: 1, the general situation of the case group and the control group: all the subjects in the study were sex, gestational age, body weight, There was no significant difference between the two groups in terms of pregnancy and birth style. There was no significant difference between the two groups in the four loci of the SP-A2 gene, rs1059046, rs17886395rs1965707, rs1965708). The genotypic frequency distribution in the full-term group was consistent with that of Hadi-Weinberg equilibrium P0.05. The main haplotypes expressed in infants were 1A0, 1A5, 1A1A1, with frequencies of 54.2 and 54.2, respectively. There was no significant difference in the frequencies of haplotypes of SP-A2 between the preterm infants and the control group. There was no significant difference in the frequencies of haplotypes between the preterm infants and the control group (P 0.05 路4), and the limit of gestational age was 32 weeks of gestational age. After grouping (gestational age 鈮，

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