發(fā)布時(shí)間：2018-02-26 20:24

  本文關(guān)鍵詞： 血清腦源性神經(jīng)營(yíng)養(yǎng)因子 哮喘 嚴(yán)重程度 兒童　出處：《石河子大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:觀察血清腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)在兒童哮喘中的表達(dá)水平,探討B(tài)DNF在兒童哮喘發(fā)病機(jī)制中的可能作用及其與哮喘患兒病情嚴(yán)重程度的關(guān)系。方法:哮喘組選取2015年7月至2016年7月于石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院兒科診治的60例哮喘急性期患兒,其中男性39例,女性21例,年齡1.3~14.0歲,平均(8.1±3.2)歲;將哮喘患兒按哮喘病情嚴(yán)重程度分為輕度組18例、中度組25例和重度組17例;對(duì)照組選取同期本院體檢的健康兒童60例,其中男性38例、女性22例,年齡1.1~14.4歲,平均(7.4±3.2)歲,并收集臨床基本資料。哮喘組兒童分別于發(fā)作期治療前和癥狀緩解期(無(wú)喘息癥狀、肺部未聞及哮鳴音、予以規(guī)范化控制治療)抽取空腹靜脈血各2ml,對(duì)照組于就診當(dāng)天取空腹外周靜脈血2ml,室溫(20~25℃)60min后3000r/min離心10min,取血清分裝于EP管中,-70℃冰箱留存待檢,避免反復(fù)凍融。應(yīng)用酶聯(lián)免疫吸附法(enzyme-linked immunosorbent assay,ELISA)檢測(cè)兩組兒童血清中BDNF的表達(dá)水平,比較不同嚴(yán)重程度的哮喘患兒血清BDNF水平的差異,分析BDNF水平與兒童哮喘病情嚴(yán)重程度的關(guān)系,同時(shí)將收集的臨床基本資料進(jìn)行統(tǒng)計(jì)學(xué)分析。數(shù)據(jù)采用SPSS 17.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)處理。計(jì)量資料以均數(shù)±標(biāo)準(zhǔn)差(?x±s)表示,正態(tài)分布且方差齊者采用t檢驗(yàn)或方差分析,方差不齊者采用非參數(shù)檢驗(yàn);計(jì)數(shù)資料以率表示,采用卡方檢驗(yàn)進(jìn)行比較;變量間的相關(guān)性采用相關(guān)性分析。P0.05為差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:1.哮喘急性發(fā)作組及癥狀緩解組的BDNF水平分別為(8.8±0.6)ng/ml、(6.4±0.9)ng/ml,均明顯高于對(duì)照組(1.9±1.2)ng/ml,以急性發(fā)作組最高(P0.05),緩解期的BDNF水平低于發(fā)作期(P0.05)。2.哮喘患兒病情嚴(yán)重程度不同,其血清BDNF水平不同:輕度組最低(8.2±0.4)ng/ml、重度組最高(9.5±0.3)ng/ml,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。哮喘病情程度越重,血清BDNF的表達(dá)水平越高,兩者呈正相關(guān)(r=0.756,P0.05)。3.哮喘組中33例具有特應(yīng)性體質(zhì),27例無(wú)特應(yīng)性體質(zhì),兩者的血清BDNF水平分別為(9.1±0.6)ng/ml、(8.4±0.5)ng/ml,進(jìn)行比較結(jié)果顯示具有特應(yīng)性體質(zhì)的哮喘患兒血清BDNF的水平更高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。相關(guān)性分析結(jié)果顯示兩者呈正相關(guān)關(guān)系(r=0.504,P0.05)。結(jié)論:BDNF可能在兒童哮喘的發(fā)病機(jī)制中起著一定的作用,與患兒的特應(yīng)性體質(zhì)有關(guān),并且其血清表達(dá)水平與哮喘病情嚴(yán)重程度相關(guān),檢測(cè)血清BDNF水平將有助于評(píng)估哮喘患兒的病情,對(duì)判斷兒童哮喘的病情發(fā)展可能有一定的作用,為兒童哮喘的防治提供新思路。
[Abstract]:Objective: To observe the effect of brain-derived neurotrophic factor (BDNF) expression level in children with asthma and investigate the relationship between the possible role of BDNF in the pathogenesis of asthma in children with asthma severity and disease. Methods: 60 cases of asthma in children with acute asthma group from July 2015 to July 2016 in the First Affiliated Hospital of Shihezi University Medical Department of Pediatrics the hospital, of which 39 were male, 21 were female, aged 1.3~14.0 years old, the average (8.1 + 3.2) years; asthma in children according to the severity of asthma were divided into mild group, 18 cases, 25 cases of moderate group and severe group 17 cases; 60 cases of control group were selected in our hospital and 38 healthy children. Cases of male, female 22 cases, age 1.1~14.4 years old, the average (7.4 + 3.2) years old, and collected basic clinical data. In asthmatic children in acute stage respectively before treatment and symptom remission (no wheezing symptoms, lung without wheeze, be. Standardized control treatment) fasting venous blood 2ml, the control group to visit the same day fasting peripheral venous blood 2ml, room temperature (20~25 C) 60min 3000r/min centrifugal 10min, serum packed in EP tubes, -70 C refrigerator retained for inspection, to avoid repeated freezing and thawing. The application of enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) to detect the expression of BDNF in serum of two groups of children, to compare the differences among different severity of asthma children serum BDNF level, and analyze the relationship between BDNF level in children with asthma severity, and clinical base collected data for statistical analysis. The data using SPSS 17 statistical software measurement. Data to mean + standard deviation (? X + s) said that the normal distribution and homogeneity of variance using the t test or analysis of variance, homogeneity of variance using non parametric test; count data expressed as a ratio, chi square test Were compared; the correlation between variables by correlation analysis.P0.05 the difference was statistically significant. Results: the symptoms of acute attack group and remission group 1. asthma BDNF levels were (8.8 + 0.6) ng/ml, (6.4 + 0.9) ng/ml, were significantly higher than the control group (1.9 + 1.2) ng/ml, with acute exacerbation group was the highest (P0.05), remission of BDNF level is lower than the onset of illness (P0.05).2. in children with asthma severity, the serum level of BDNF in different groups: mild minimum (8.2 + 0.4) ng/ml, the highest in severe group (9.5 + 0.3) ng/ml, the difference was statistically significant (P0.05). Asthma severity, expression the serum level of BDNF is high. There is a positive correlation between them (r=0.756, P0.05).3. in the asthma group of 33 patients with atopy, 27 cases without atopy, serum BDNF levels of the two respectively (9.1 + 0.6) ng/ml, (8.4 + 0.5) ng/ ml, the results were compared with that of atopic children with asthma constitution The serum levels of BDNF were higher, the difference was statistically significant (P0.05). The results of correlation analysis showed that there was a positive correlation between (r=0.504, P0.05). Conclusion: plays a certain role in the pathogenesis of BDNF in childhood asthma, associated with atopy physique, and the expression level of serum and severe asthma the degree of correlation, serum BDNF level will help children with asthma severity assessment, to determine the development of asthma in children disease may have a certain effect, to provide new ideas for prevention and treatment of asthma in children.

【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R725.6

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本文編號(hào)：1539574