本文關(guān)鍵詞： 七味白術(shù)散 輪狀病毒腸炎 腸道菌群 宏基因組學(xué)　出處：《廣州中醫(yī)藥大學(xué)》2014年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的： 利用宏基因組學(xué)方法,通過(guò)動(dòng)物及臨床實(shí)驗(yàn),研究七味白術(shù)散治療輪狀病毒(RV)腸炎中對(duì)腸道宏基因組的影響,以利于探索腸道微生態(tài)在中藥治療輪狀病毒腸炎中的可能作用。 方法： 本項(xiàng)研究包括動(dòng)物實(shí)驗(yàn)及臨床實(shí)驗(yàn)兩階段。在動(dòng)物實(shí)驗(yàn)階段中,選擇5日齡昆明小鼠為研究對(duì)象,按窩別隨機(jī)分為七味白術(shù)散組、模型對(duì)照組及健康對(duì)照組；七味白術(shù)散組及模型對(duì)照組均以輪狀病毒(RV)懸液經(jīng)口感染,造模成功后七味白術(shù)散組以七味白術(shù)散復(fù)方煎液經(jīng)口灌胃,模型對(duì)照組及健康對(duì)照組則喂以等量的生理鹽水；3日后采集各組乳鼠的結(jié)腸內(nèi)容物標(biāo)本,并應(yīng)用宏基因組學(xué)方法,檢測(cè)及對(duì)比其腸道菌群結(jié)構(gòu)。在臨床實(shí)驗(yàn)階段中,選取1例辨證為脾虛證的12個(gè)月大的輪狀病毒腸炎患兒,應(yīng)用宏基因組學(xué)方法,檢測(cè)及對(duì)比其服用七味白術(shù)散前后腸道菌群的變化。 結(jié)果： 動(dòng)物和臨床實(shí)驗(yàn)結(jié)果顯示,RV感染后的乳鼠和嬰兒之間,其腸道菌群結(jié)構(gòu)差異很大,例如,在門(mén)的水平上,前者以變形菌(Proteobacteria)為主,而后者則以放線(xiàn)菌(Actinobacteria)為主。七味白術(shù)散治療后的RV感染乳鼠與模型對(duì)照組、健康對(duì)照組之間,以及七味白術(shù)散治療后的RV感染嬰兒前后,其腸道菌群結(jié)構(gòu)均存在顯著不同。對(duì)選擇的腸道關(guān)鍵菌群——雙歧桿菌和乳桿菌,在動(dòng)物實(shí)驗(yàn)中,七味白術(shù)散組的腸道雙歧桿菌含量高于模型對(duì)照組,而乳桿菌含量則顯著低于模型對(duì)照組；然而,對(duì)RV感染嬰兒,其腸道雙歧桿菌含量在七味白術(shù)散治療后顯著下降,而乳桿菌含量則在治療后顯著增高。 結(jié)論： 七味白術(shù)散的治療改變了RV感染乳鼠和嬰兒的腸道菌群結(jié)構(gòu),但很可能并不是通過(guò)影響單一特定菌群來(lái)起作用；另一方面,由于乳鼠和嬰兒之間的腸道菌群結(jié)構(gòu)差異很大,功能基因組學(xué)的分析可能更有價(jià)值。
[Abstract]:Objective: The effects of Qiwei Baizhu Powder on intestinal macrogenome in the treatment of rotavirus RV) enteritis were studied by means of macrogenomics and animal and clinical experiments. In order to explore the role of intestinal microecology in the treatment of rotavirus enteritis. Methods: This study includes two stages: animal experiment and clinical experiment. In the animal experiment stage, the 5-day-old Kunming mice were selected as the research objects, and randomly divided into Qiwei Baizhu powder group, model control group and healthy control group according to their nest; Qiwei Baizhu Powder group and model control group were infected by rotavirus RV) suspension. After the successful establishment of the model, Qiwei Baizhu Powder group was orally perfused with Qiwei Baizhu Powder compound decoction. The model control group and the healthy control group were fed with the same amount of normal saline. 3 the colonic contents of each group of neonatal rats were collected, and the intestinal microflora structure was detected and compared by means of macrogenomics. A case of rotavirus enteritis aged 12 months with spleen deficiency syndrome was selected to detect and compare the changes of intestinal flora before and after taking Qiwei Baizhu Powder by macrogenomics. Results: Animal and clinical trials have shown that the intestinal flora structure varies significantly between newborn mice and infants infected with RV, for example, at the portal level. The former is Proteobacteria (Proteobacteria). The latter was mainly actinobacteriae). After treatment with Qiwei Atractylodes powder, there were three groups: model control group, model control group and healthy control group. The intestinal flora structure of infants infected with RV after treatment with Qiwei Baizhu Powder was significantly different. The key intestinal flora bifidobacterium and Lactobacillus were selected in animal experiment. The intestinal bifidobacterium content in Qiwei Baizhu Powder group was higher than that in the model control group, while the Lactobacillus content was significantly lower than that in the model control group. However, for infants infected with RV, the intestinal bifidobacterium content decreased significantly after treatment with Qiwei Baizhu Powder, while the content of Lactobacillus increased significantly after treatment. Conclusion: The treatment of Qiwei Baizhu Powder changed the intestinal microflora structure of newborn mice and infants infected with RV, but probably not by affecting a single specific flora. On the other hand, the analysis of functional genomics may be more valuable because of the large differences in intestinal flora structure between newborn mice and infants.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R272

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 丁維俊,周邦靖,馮立秀,王宇;脾虛造模及參苓白術(shù)散的真菌菌群調(diào)整功能[J];成都中醫(yī)藥大學(xué)學(xué)報(bào);2004年01期

2 張文;;參苓白術(shù)散加減治療小兒腹瀉45例臨床報(bào)告[J];當(dāng)代醫(yī)學(xué);2009年07期

3 伍參榮,楊波,胡建中,趙武能,盧芳國(guó);七味白術(shù)散對(duì)人輪狀病毒感染乳鼠胸腺細(xì)胞的程序性死亡和IL-2、IL-2R表達(dá)的影響[J];湖南中醫(yī)學(xué)院學(xué)報(bào);2001年03期

4 褚家秀;曾藝文;;七味白術(shù)散聯(lián)合思密達(dá)治療小兒秋季腹瀉43例[J];江西中醫(yī)藥;2006年02期

5 陳宇,王禹,楊景云;嬰幼兒輪狀病毒腸炎腸道微生態(tài)學(xué)改變[J];黑龍江醫(yī)藥科學(xué);2001年01期

6 尹應(yīng)先;肖密絲;歐志英;朱冰;;2005-2007年廣州地區(qū)腹瀉兒童感染輪狀病毒情況分析[J];熱帶醫(yī)學(xué)雜志;2009年07期

7 王麗媛;謝詠梅;汪志凌;高珊;;益生菌調(diào)節(jié)輪狀病毒腸炎患兒腸道菌群的動(dòng)態(tài)變化[J];實(shí)用兒科臨床雜志;2011年07期

8 伍參榮,謝朝暉,賀雙騰,楊波,胡建中,趙武能,盧芳國(guó);七味白術(shù)散對(duì)HRV感染乳鼠NK、IFN-γ、IL-4的影響[J];中國(guó)中醫(yī)藥信息雜志;2002年04期

9 姜曉;伍參榮;田雪飛;賀雙藤;李杰;李珊;蔡銳;陳伶俐;高立凡;;七味白術(shù)散對(duì)人類(lèi)輪狀病毒感染乳鼠小腸黏膜上皮細(xì)胞的保護(hù)作用[J];中國(guó)中醫(yī)藥信息雜志;2010年05期

10 謝華萍;耿進(jìn)妹;伍業(yè)健;魏躍紅;魯恩潔;狄飚;楊智聰;;2009年廣州地區(qū)輪狀病毒分子流行病學(xué)研究[J];預(yù)防醫(yī)學(xué)情報(bào)雜志;2011年10期

，

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