發(fā)布時(shí)間：2018-01-19 05:32

  本文關(guān)鍵詞： 睪丸扭轉(zhuǎn) 缺血再灌注 減壓術(shù) 比較　出處：《大理學(xué)院》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:建立雄兔睪丸扭轉(zhuǎn)復(fù)位模型，探討睪丸扭轉(zhuǎn)復(fù)位中睪丸內(nèi)減壓和缺血再灌注間的聯(lián)系，對(duì)照研究單純復(fù)位和減容復(fù)位對(duì)睪丸扭轉(zhuǎn)復(fù)位的影響并評(píng)價(jià)其預(yù)后，為睪丸扭轉(zhuǎn)復(fù)位后的干預(yù)提供新的思路。并就一側(cè)睪丸受損是否對(duì)對(duì)側(cè)睪丸產(chǎn)生影響進(jìn)行探討。 方法:18只雄性大白兔隨機(jī)分成三組：假手術(shù)組（A）、單純復(fù)位組（B組）、減容復(fù)位組(C組),每組6只。B組、C組建立雄兔睪丸扭轉(zhuǎn)模型后行超聲學(xué)檢查，B組單純復(fù)位（干預(yù)側(cè)為B1、對(duì)側(cè)B2），C組減容復(fù)位（干預(yù)側(cè)為C1、對(duì)側(cè)C2）,1d后測(cè)定各組睪丸組織超氧化物歧化酶(SOD)含量；HE染色后光鏡下觀察睪丸組織的病理學(xué)改變,并行Johnsen's評(píng)分；1w超聲檢查，處死雄兔，同樣測(cè)定SOD,行病理學(xué)檢查。 結(jié)果:精索扭轉(zhuǎn)后4h后彩色多普勒超聲檢查睪丸血供減少至術(shù)前的35％－50％，組織超氧化物歧化酶(SOD)含量和Johnsen 's評(píng)分分別是：（106.4±8.16）U/mg、（9.13±0.16）分，，與假手術(shù)A比較有顯著差異（P0.05）,睪丸扭轉(zhuǎn)模型建立成功。1w后各組彩色多普勒超聲檢查睪丸血供減少至術(shù)前的分別是：B1組10％～25％，C1組30％～50％，B2組90～95％，C1血供比B1明顯豐富、B2血供與A比較略減少；組織SOD含量:1d和1w分別為：B1（98.20±9.95）U/mg、（83.28±10.00）U/mg，C1（110.91±12.16）U/mg、（100.78±11.30）U/mg；B1，C1與A比較有顯著差異（P0.05），B1與C1比較有顯著差異（P0.05）。對(duì)側(cè)：B2（128.90±8.39）U/mg、（116.16±9.34）U/mg，C2（129.87±8.94）U/mg、（134.40±8.17）U/mg；1d各組比較無(wú)統(tǒng)計(jì)學(xué)意義（P0.05）；1w：A與B2比較有顯著差異（P0.05）；A與C2比較無(wú)顯著差異(P0.05)；C2與B2比較有顯著差異（P0.05）。Johnsen 's評(píng)分:1d和1w.B1分別為（8.49±0.18）分、（4.95±0.43）分，C1（8.93±0.08）分、（7.76±0.33）分,B1.C1與A比較有顯著差異（P0.05），C1與B1比較有顯著差異(P 0.05)。對(duì)側(cè)B2與C2比較無(wú)顯著差異(P0.05),但有形態(tài)學(xué)的改變。 結(jié)論:睪丸扭轉(zhuǎn)復(fù)位缺血再灌注后隨著時(shí)間越長(zhǎng)，損傷越明顯；睪丸內(nèi)減壓方法可更有利于扭轉(zhuǎn)復(fù)位側(cè)睪丸的恢復(fù)；一側(cè)睪丸受損隨著時(shí)間的延長(zhǎng)對(duì)對(duì)側(cè)睪丸也有一定影響，減容復(fù)位方可較單純復(fù)位有效緩解對(duì)對(duì)側(cè)睪丸的損傷。
[Abstract]:Objective: to establish a model of testicular torsion reduction in male rabbits, and to explore the relationship between intratesticular decompression and ischemia-reperfusion during testicular torsion reduction. To study the effect of simple reduction and volume reduction on testicular torsion reduction and evaluate its prognosis. To provide a new idea for the intervention after testicular torsion reduction, and to discuss whether the unilateral testicular injury has an effect on the contralateral testis. Methods 18 male white rabbits were randomly divided into three groups: sham-operated group, simple reduction group, and reduced volume reduction group, with 6 rats in each group. After the establishment of testicular torsion model in group C, simple reduction was performed in group B (intervention side was B1, contralateral group B _ 2 was reduced by volume reduction) (intervention side was C _ 1, contralateral C _ 2). The content of superoxide dismutase (SOD) in testicular tissue of each group was measured 1 day later. After HE staining, the pathological changes of testis were observed under light microscope, and Johnsen's score was used. After 1 week ultrasound examination, male rabbits were killed, SOD was also measured and pathological examination was made. Results: four hours after spermatic torsion, the testicular blood supply was reduced to 35-50% by color Doppler ultrasonography. The content of superoxide dismutase (SOD) and the Johnsen score were 106.4 鹵8.16U / mg / m ~ (-1) 鹵9.13 鹵0.16, respectively. Compared with sham-operation A, there was a significant difference (P0.05%). After the establishment of testicular torsion model, the blood supply of testicular blood was reduced to 25% in group B _ 1 after the establishment of testicular torsion model. The blood supply of C _ 1 in group C _ 1 was significantly richer than that of B _ 1 in group B _ 2, and the blood supply of group B _ 2 was slightly lower than that of group A; The SOD content of the tissue was 98.20 鹵9.95 U / mg for 1 d and 83.28 鹵10.00 U / mg for 1 day and 1 week, respectively. C1C 110.91 鹵12.16 U / MGN 100.78 鹵11.30 U / mg; There was significant difference between B1C 1 and A (P 0.05) and C 1 (P 0.05). The contralateral ratio of B2C 1 was 128.90 鹵8.39 U / mg. 116.16 鹵9.34 U / mgt C2N 129.87 鹵8.94 U / MGN 134.40 鹵8.17U / mg; On the 1st day, there was no significant difference in each group (P 0.05). 1 w: a was significantly different from B2 (P 0.05); There was no significant difference between A and C2 (P 0.05). There was significant difference between C2 and B2 (P 0.05). Johnsen's scores of 1 d and 1w.B1 were 8.49 鹵0.18 and 4.95 鹵0.43, respectively. There was a significant difference between C _ (1) and A (P _ (0.05)) and C _ (1) (8.93 鹵0.08) and B _ (1.C1), respectively (7.76 鹵0.33). There was significant difference between C1 and B1 (P0.05), but there was no significant difference between the contralateral B2 and C2, but there were morphological changes. Conclusion: the longer the time is, the more obvious the injury is after testicular torsion reduction and ischemia reperfusion. The method of intratesticular decompression is more beneficial to the recovery of the torsional reduction side of testis. Unilateral testicular damage also had some effect on contralateral testis with the extension of time. Volume reduction and reduction were more effective than simple reduction to alleviate the damage to contralateral testis.
【學(xué)位授予單位】：大理學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R726.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 苗文隆;張曉云;;鈣通道拮抗劑對(duì)大鼠睪丸缺血再灌注損傷保護(hù)作用的研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2006年17期

本文編號(hào)：1442874