【摘要】：隨著規(guī)模養(yǎng)豬業(yè)的發(fā)展,規(guī)�；B(yǎng)豬場的疫病種類和數(shù)量均有增加的趨勢,從而對養(yǎng)豬業(yè)造成巨大的損失。為了了解規(guī)�；i場中主要疫病的感染情況,本人采用PCR和RT-PCR方法,結(jié)合細(xì)菌分離鑒定,2014年8月至2015年3月,對某集團(tuán)公司下屬分別來自福建、廣西、江西、上海、安徽5個(gè)省(市)的14個(gè)規(guī)模化豬場的126份組織樣品(發(fā)病豬的腦、肺臟、脾臟、腎臟、心臟、淋巴結(jié)、關(guān)節(jié)積液等)分別進(jìn)行了8種病原檢測。結(jié)果顯示:14個(gè)規(guī)模化豬場,86份樣品均有不同病原的感染,豬場和組織樣品的陽性率分別為100%(14/14)和68.25%(86/126)。其中,單獨(dú)或混合感染豬圓環(huán)病毒2型(PCV2)、豬繁殖與呼吸綜合征病毒(PRRSV)、豬瘟病毒(CSFV)、豬偽狂犬病毒(PRV)、豬肺炎支原體(Mhp)、豬傳染性胸膜肺炎放線桿菌(APP)、豬鏈球菌(SS)、副豬嗜血桿菌(HPS)的陽性樣品分別為50.79%(64/126)、24.6%(21/126)、7.14%(9/126)、0(0/126)、16.67%(21/126)、8.73%(11/126)、11.11%(14/126)、7.14%(9/126)。從感染的類型看,在86份陽性樣品中,單獨(dú)感染的樣品31份,占36.05%(31/86);混合感染的樣品55份,占63.95%(55/86)。從混合感染的類型看,在55份混合感染陽性樣品中,二重混合感染39份、三重混合感染15份、四重混合感染1份,分別占70.91%(39/55)、27.27%(15/55)和1.82%(1/55)。從混合感染的微生物種類看,在55份混合感染陽性樣品中,“病毒與病毒”混合感染的樣品19份、“病毒與細(xì)菌”混合感染的樣品36份,分別占34.55%(19/55)和65.45%(36/55)。從感染的優(yōu)勢病原分析,PCV2和/或PRRSV感染的樣品75份,占86份陽性樣品的87.21%。其中,“PCV2+PRRSV(+其它病原)”混合感染的樣品20份,占所有55份混合感染樣品的36.36%。結(jié)果表明:該集團(tuán)公司下屬規(guī)模豬場普遍存在病毒與細(xì)菌感染;感染類型復(fù)雜多樣,混合感染相當(dāng)普遍,以“PCV2十PRRSV(+其它病原)”混合感染最為常見。對分離得到的11株APP、14株SS、9株HPS進(jìn)行藥物敏感性試驗(yàn)。結(jié)果表明3種分離菌對14種抗菌素的敏感性不同,其中SS與HPS對頭孢噻肟與頭孢曲松鈉敏感度極高,而APP對恩諾沙星敏感性極高。
[Abstract]:With the development of large-scale pig industry, the species and quantity of epidemic diseases in large-scale pig farms have an increasing trend, which causes great losses to pig industry. In order to understand the infection of the main epidemic diseases in large-scale pig farms, I used PCR and RT-PCR methods, combined with bacterial isolation and identification. From August 2014 to March 2015, 126 tissue samples (brain, lung, spleen, kidney, heart, lymph nodes) from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (cities) were collected from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (including brain, lung, spleen, kidney, heart and lymph nodes). Eight kinds of pathogens were detected respectively. The results showed that 86 samples from 14 large-scale pig farms were infected with different pathogens. The positive rates of pig farms and tissue samples were 100% (14 鈮，

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