【摘要】：李氏桿菌病(Listeriosis)是一種危害嚴(yán)重的食源性人畜共患傳染病,單核細(xì)胞增多性李氏桿菌(Listeria monocytogenes,LM)簡(jiǎn)稱單增李氏桿菌是導(dǎo)致其引發(fā)疾病的主要病原菌。現(xiàn)已證實(shí)該菌可感染40多種動(dòng)物,主要可引起人和動(dòng)物出現(xiàn)敗血癥、流產(chǎn)、腦膜炎等癥狀。近幾十年,隨著李氏桿菌病在世界范圍內(nèi)的不斷暴發(fā),已受到廣泛關(guān)注。因此對(duì)各地區(qū)的綿羊進(jìn)行單增李氏桿菌的分離鑒定,并建立單增李氏桿菌病的快速檢測(cè)技術(shù),對(duì)單增李氏桿菌病的預(yù)防和治療具有重要的意義。根據(jù)國(guó)家標(biāo)準(zhǔn)(GB4789.30-2010)對(duì)伊犁部分地區(qū)(昭蘇縣、尼勒克縣、霍城縣、察布查爾縣)羊場(chǎng)的綿羊、青貯飼料進(jìn)行隨機(jī)采樣,通過培養(yǎng)特性、生化特性對(duì)分離株進(jìn)行鑒定。利用伊犁地區(qū)綿羊體內(nèi)分離鑒定的單增李氏桿菌作為平板凝集抗原,與標(biāo)準(zhǔn)陽性血清進(jìn)行平板凝集試驗(yàn),建立平板凝集檢測(cè)方法,并通過特異性試驗(yàn)和符合性試驗(yàn)進(jìn)行驗(yàn)證。結(jié)果從綿羊體內(nèi)及青貯飼料中分離出14株單增李氏桿菌,表明抽查地區(qū)羊場(chǎng)的綿羊體內(nèi)和青貯飼料中均存在一定程度的感染或污染。平板凝集試驗(yàn)確定平板凝集抗原最佳工作濃度為7×109CFU/m L,通過特異性試驗(yàn)證驗(yàn)該方法具有很好的特異性,符合性試驗(yàn)的總符合率為93.4%。研究結(jié)果表明伊犁地區(qū)的綿羊有發(fā)生單增李氏桿菌病的潛在威脅,建立的平板凝集方法可較為快速、準(zhǔn)確的檢測(cè)單增李氏桿菌病。以上研究結(jié)果可為伊犁地區(qū)綿羊的單增李氏桿菌病預(yù)防和監(jiān)控提供科學(xué)的理論依據(jù)。
[Abstract]:Listeria monocytogenes (Listeriosis) is a serious food-borne zoonotic infectious disease. Listeria monocytogenes (Listeria monocytogenes,LM) is the main pathogen causing the disease. It has been confirmed that the bacteria can infect more than 40 kinds of animals, mainly causing septicemia, abortion, meningitis and other symptoms in humans and animals. In recent decades, with the continuous outbreak of listeriasis in the world, it has been widely concerned. Therefore, it is of great significance for the prevention and treatment of Listeria monocytogenes to isolate and identify Listeria monocytogenes in sheep and to establish a rapid detection technique for Listeria monocytogenes. According to the national standard (GB4789.30-2010), the sheep and silage of sheep farms in some areas of Yili (Zhaosu County, Nilek County, Huocheng County, Chabuchal County) were randomly sampled, and the isolates were identified by culture characteristics and biochemical characteristics. Using Listeria monocytogenes isolated and identified in Yili sheep as plate agglutination antigen, plate agglutination test was carried out with standard positive serum, and the plate agglutination detection method was established, which was verified by specific test and conformity test. Results 14 strains of Listeria monocytogenes were isolated from sheep and silage, which indicated that there was a certain degree of infection or pollution in sheep and silage in sheep farms. The optimum working concentration of plate agglutination antigen was determined to be 7 脳 109CFU/m / L by plate agglutination test. The method was proved to be very specific by specific test, and the total coincidence rate of conformance test was 93.4%. The results showed that sheep in Yili area had the potential threat of listeria monocytogenes, and the plate agglutination method could be used to detect listeria monocytogenes quickly and accurately. The above results can provide a scientific theoretical basis for the prevention and monitoring of listeria monocytogenes in sheep in Yili area.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.26

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