【摘要】：李氏桿菌病(Listeriosis)是一種危害嚴(yán)重的食源性人畜共患傳染病,單核細胞增多性李氏桿菌(Listeria monocytogenes,LM)簡稱單增李氏桿菌是導(dǎo)致其引發(fā)疾病的主要病原菌�，F(xiàn)已證實該菌可感染40多種動物,主要可引起人和動物出現(xiàn)敗血癥、流產(chǎn)、腦膜炎等癥狀。近幾十年,隨著李氏桿菌病在世界范圍內(nèi)的不斷暴發(fā),已受到廣泛關(guān)注。因此對各地區(qū)的綿羊進行單增李氏桿菌的分離鑒定,并建立單增李氏桿菌病的快速檢測技術(shù),對單增李氏桿菌病的預(yù)防和治療具有重要的意義。根據(jù)國家標(biāo)準(zhǔn)(GB4789.30-2010)對伊犁部分地區(qū)(昭蘇縣、尼勒克縣、霍城縣、察布查爾縣)羊場的綿羊、青貯飼料進行隨機采樣,通過培養(yǎng)特性、生化特性對分離株進行鑒定。利用伊犁地區(qū)綿羊體內(nèi)分離鑒定的單增李氏桿菌作為平板凝集抗原,與標(biāo)準(zhǔn)陽性血清進行平板凝集試驗,建立平板凝集檢測方法,并通過特異性試驗和符合性試驗進行驗證。結(jié)果從綿羊體內(nèi)及青貯飼料中分離出14株單增李氏桿菌,表明抽查地區(qū)羊場的綿羊體內(nèi)和青貯飼料中均存在一定程度的感染或污染。平板凝集試驗確定平板凝集抗原最佳工作濃度為7×109CFU/m L,通過特異性試驗證驗該方法具有很好的特異性,符合性試驗的總符合率為93.4%。研究結(jié)果表明伊犁地區(qū)的綿羊有發(fā)生單增李氏桿菌病的潛在威脅,建立的平板凝集方法可較為快速、準(zhǔn)確的檢測單增李氏桿菌病。以上研究結(jié)果可為伊犁地區(qū)綿羊的單增李氏桿菌病預(yù)防和監(jiān)控提供科學(xué)的理論依據(jù)。
[Abstract]:Listeria monocytogenes (Listeriosis) is a serious food-borne zoonotic infectious disease. Listeria monocytogenes (Listeria monocytogenes,LM) is the main pathogen causing the disease. It has been confirmed that the bacteria can infect more than 40 kinds of animals, mainly causing septicemia, abortion, meningitis and other symptoms in humans and animals. In recent decades, with the continuous outbreak of listeriasis in the world, it has been widely concerned. Therefore, it is of great significance for the prevention and treatment of Listeria monocytogenes to isolate and identify Listeria monocytogenes in sheep and to establish a rapid detection technique for Listeria monocytogenes. According to the national standard (GB4789.30-2010), the sheep and silage of sheep farms in some areas of Yili (Zhaosu County, Nilek County, Huocheng County, Chabuchal County) were randomly sampled, and the isolates were identified by culture characteristics and biochemical characteristics. Using Listeria monocytogenes isolated and identified in Yili sheep as plate agglutination antigen, plate agglutination test was carried out with standard positive serum, and the plate agglutination detection method was established, which was verified by specific test and conformity test. Results 14 strains of Listeria monocytogenes were isolated from sheep and silage, which indicated that there was a certain degree of infection or pollution in sheep and silage in sheep farms. The optimum working concentration of plate agglutination antigen was determined to be 7 脳 109CFU/m / L by plate agglutination test. The method was proved to be very specific by specific test, and the total coincidence rate of conformance test was 93.4%. The results showed that sheep in Yili area had the potential threat of listeria monocytogenes, and the plate agglutination method could be used to detect listeria monocytogenes quickly and accurately. The above results can provide a scientific theoretical basis for the prevention and monitoring of listeria monocytogenes in sheep in Yili area.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.26

【參考文獻】

相關(guān)期刊論文 前10條

1 馬世春;蘇增華;馬繼紅;董浩;徐一;魏巍;孫雨;池麗娟;王曉英;謝巧;肖開提·阿不都克里木;;李氏桿菌病[J];中國畜牧業(yè);2015年05期

2 王光芳;丁明彬;丁維亮;黃秉政;;李斯特菌病的防治[J];中國畜禽種業(yè);2012年10期

3 王文豪;章勝;張丹琳;蘇丹萍;賀東生;;副豬嗜血桿菌玻板凝集抗原的研制[J];養(yǎng)豬;2012年03期

4 唐新琳;張樹輝;;李斯特菌主要毒力及其致病機理[J];華章;2012年02期

5 趙新斐;尚文婧;王靜梅;剡根強;;新疆綿羊及其環(huán)境中李氏桿菌的生態(tài)分布多樣性調(diào)查[J];畜牧與獸醫(yī);2012年01期

6 田靜;樊永祥;劉秀梅;;散裝熟肉制品中單核細胞增生李斯特菌的定量風(fēng)險評估[J];中華預(yù)防醫(yī)學(xué)雜志;2011年06期

7 劉海泉;沈瀟;吳啟華;潘迎捷;孫曉紅;;單核細胞增生性李斯特氏菌快速檢測技術(shù)研究進展[J];湖南農(nóng)業(yè)科學(xué);2011年01期

8 呂亞楠;李影;王偉麗;孟慶峰;錢愛東;;單核增生性李氏桿菌病研究進展[J];吉林農(nóng)業(yè);2010年11期

9 羅雁非;賈芙蓉;丁旭;方玲;鄭嵐;何成彥;;膠體金標(biāo)記單克隆抗體測定單增李斯特菌試劑盒制備[J];中國實驗診斷學(xué);2009年09期

10 李磊;婁興國;李永強;李�；�;;雞白痢沙門氏菌平板凝集抗原的制備[J];河南畜牧獸醫(yī)(綜合版);2008年09期

，

本文編號：2501259