【摘要】：選擇不同飼養(yǎng)條件下12月齡蘇尼特羊的背最長(zhǎng)肌為實(shí)驗(yàn)材料,通過(guò)測(cè)定4種miRNAs的表達(dá)量與肉品質(zhì),研究不同飼養(yǎng)方式對(duì)miRNAs表達(dá)量及肉品質(zhì)的影響。結(jié)果表明,放牧蘇尼特羊背最長(zhǎng)肌嫩度高于圈養(yǎng),而圈養(yǎng)紅度值顯著高于放牧(P0.05)。圈養(yǎng)蘇尼特羊背最長(zhǎng)肌pH_2著高于放牧(P0.05)。2種飼養(yǎng)方式下,4個(gè)miRNA均有表達(dá),且放牧蘇尼特羊背最長(zhǎng)肌miRNAs基因表達(dá)量高于圈養(yǎng)。蘇尼特羊miR-30a與紅度值呈顯著負(fù)相關(guān)(P0.05)。不同飼養(yǎng)方式對(duì)miR-128、miR-486、miR-30a、miR-223的表達(dá)有影響,并進(jìn)一步影響肉的品質(zhì)。
[Abstract]:The longissimus dorsi muscle of 12-month-old Sunita sheep was selected as experimental material. The effects of different feeding methods on the expression of miRNAs and meat quality were studied by measuring the expression of four kinds of miRNAs and meat quality. The results showed that the longest muscle tenderness of the back of grazing Sunita sheep was higher than that of captive culture, and the redness of captive culture was significantly higher than that of grazing (P 0.05). The pH_2 of the longissimus dorsi muscle of Sunita sheep in captivity was higher than that of grazing (P 0.05). Under the two feeding methods, the expression of miRNAs gene in the longissimus dorsi muscle of grazing Sunita sheep was higher than that in captivity. There was a significant negative correlation between miR-30a and redness in Sunita sheep (P 0.05). Different feeding methods had an effect on the expression of miR-128,miR-486,miR-30a,miR-223 and further affected the quality of meat.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院;
【基金】：高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金資助項(xiàng)目(20121515110001) 國(guó)家自然科學(xué)基金資助項(xiàng)目(31360393,31260378)
【分類號(hào)】：S826

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