【摘要】：本課題研究日糧不同鈷添加水平對(duì)生長(zhǎng)獺兔生產(chǎn)性能、代謝性能和毛囊發(fā)育的影響。試驗(yàn)選取基線水平一致的生長(zhǎng)獺兔200只,隨機(jī)分為5組,分別飼喂不同鈷添加水平(0、0.1、0.4、1.6、6.4mg/kg)的試驗(yàn)日糧,采用單因素試驗(yàn)設(shè)計(jì)方法研究其對(duì)生長(zhǎng)獺兔的影響。試驗(yàn)結(jié)果如下:(1)在初始體重(IBW)無(wú)顯著差異的前提下,日糧鈷添加水平對(duì)試驗(yàn)?zāi)┢隗w重(EBW)有顯著影響(P0.05),對(duì)平均日采食量(ADI)(P0.01)有極顯著影響。對(duì)平均日增重(ADG)、料重比(F/G)、全凈膛率、半凈膛率、肉品質(zhì)、胸腺指數(shù)、脾指數(shù)、肝指數(shù)、腸絨毛高度、隱窩深度、V/C均無(wú)顯著影響(P0.05),隨著日糧鈷添加水平的升高,EBW、ADI、半凈膛率、胸腺指數(shù)、十二指腸絨毛高度均先升高后降低,并且都在0.4 mg/kg時(shí)最高。(2)日糧鈷添加水平對(duì)糞氮(FN)、可消化氮(DN)、DN/食入氮(IN)、沉積氮(RN)/DN、總蛋白和白蛋白均無(wú)顯著影響(P0.05),對(duì)IN、UN、RN、RN/IN有顯著影響(P0.05),并且隨著日糧鈷添加水平的升高,IN、RN和RN/IN先降低后升高,UN顯著降低,在鈷添加水平為0.4~1.6 mg/kg時(shí)達(dá)到最大值。日糧鈷添加水平對(duì)胰蛋白酶活有顯著影響(P0.05),在鈷添加水平為1.6 mg/kg時(shí)最高。日糧鈷添加水平對(duì)血清尿素含量有顯著影響(P0.05),隨著日糧鈷添加水平的升高,血清尿素先降低后升高,在鈷添加水平為1.6 mg/kg時(shí)最低。日糧鈷添加水平對(duì)糞、尿、肌肉、脾臟和腎臟中鈷含量有顯著影響(P0.05),并且隨著日糧鈷添加水平的升高,鈷含量均顯著增加。(3)日糧鈷添加水平對(duì)獺兔的皮張面積、皮張重量、皮張厚度沒(méi)有顯著影響(P0.05)。隨著日糧鈷添加水平的升高,皮張重量呈現(xiàn)先增加后降低的趨勢(shì),且在鈷添加水平為0.4mg/kg時(shí)最高。日糧鈷添加水平對(duì)獺兔的被毛長(zhǎng)度、毛囊密度有顯著性影響(P0.05),隨著日糧鈷添加水平的升高,毛囊密度呈現(xiàn)先增加后降低的趨勢(shì),鈷添加水平為6.4mg/kg時(shí)毛囊密度最低可能是因?yàn)殁捥砑恿刻邔?dǎo)致輕微中毒。分別選取鈷添加量為0和6.4 mg/kg的組進(jìn)行熒光定量分析和Western Blotting技術(shù)分析,結(jié)果發(fā)現(xiàn)日糧鈷添加水平對(duì)Tyrosinase、BMP2、BMP4有顯著性影響(P0.05),并且高鈷日糧組比低鈷日糧組表達(dá)水平都升高,表明Tyrosinase、BMP2、BMP4參與了鈷對(duì)毛囊的調(diào)控,同時(shí)AMPK、Wntlob信號(hào)通路沒(méi)有參與鈷對(duì)毛囊的調(diào)控。綜合本試驗(yàn)測(cè)定指標(biāo),生長(zhǎng)獺兔日糧鈷適宜添加水平為0.4 mg/kg(實(shí)際日糧中鈷含量為0.60 mg/kg),其中鈷添加量為6.4mg/kg時(shí)獺兔有輕微中毒癥狀。
[Abstract]:In this study, the effects of different dietary cobalt supplementation levels on the performance, metabolic performance and hair follicles development of growing Rex rabbits were studied. 200 growing Rex rabbits with the same baseline level were randomly divided into 5 groups and fed with different cobalt supplementation levels (0, 0.1, 0.4, 1.6, 6.4 mg / kg). The effect of single factor design on the growth of Rex rabbits was studied. The results were as follows: (1) on the premise that there was no significant difference in initial body weight (IBW), dietary cobalt supplementation level had a significant effect on body weight (EBW) at the end of the experiment (P 0.05). It had a significant effect on the average daily feed intake (ADI) (P 0.01). There were no significant effects on average daily gain (ADG), feed weight ratio (F 鈮，

本文編號(hào)：2492607