【摘要】：試驗(yàn)旨在研究拜城油雞公雞中脂肪型脂肪酸結(jié)合蛋白(adipocyte fatty acid binding protein,A-FABP)基因的多態(tài)性及其與生產(chǎn)性能的相關(guān)性,尋找可用于拜城油雞選育的分子遺傳標(biāo)記。隨機(jī)選取92只6周齡拜城油雞公雞,采用PCR-RFLP方法檢測A-FABP基因的多態(tài)性。每種基因型選取15只體重相近的個(gè)體隨機(jī)分為3組,每組每種基因型個(gè)體各5只,分別在12、18、24周齡時(shí)屠宰并對生產(chǎn)性能指標(biāo)進(jìn)行測定。結(jié)果顯示,在拜城油雞公雞中檢測到A-FABP基因有3種基因型:AA、AB、BB,BB為優(yōu)勢基因型,B為優(yōu)勢等位基因,不符合哈代-溫伯格平衡定律(P0.01)。12周齡時(shí),各基因型間各生產(chǎn)性能指標(biāo)差異均不顯著(P0.05);18周齡時(shí),BB基因型肌間脂寬顯著高于AA基因型(P0.05),BB和AB基因型腹脂重極顯著高于AA基因型(P0.01),腹脂率顯著高于AA基因型(P0.05);24周齡時(shí),BB基因型體重、肌間脂寬、腹脂重均顯著高于AA基因型(P0.05)。推測A-FABP基因可以作為與拜城油雞公雞脂肪性狀相關(guān)的候選標(biāo)記之一。
[Abstract]:The purpose of the experiment was to study the polymorphism of fat fatty acid binding protein (adipocyte fatty acid binding protein,A-FABP) gene in Baicheng oil chicken rooster and its correlation with production performance, and to find out the molecular genetic markers which can be used in the breeding of Baicheng oil chicken. 92 6-week-old Baicheng oil chickens were randomly selected and the polymorphism of A-FABP gene was detected by PCR-RFLP. Fifteen individuals with similar body weight were randomly divided into 3 groups, 5 individuals in each group were slaughtered at the age of 12, 18 and 24 weeks, and the performance indexes were measured. The results showed that there were three genotypes of A-FABP gene in Baicheng oil chicken rooster: AA,AB,BB,BB was the dominant genotype and B was the dominant allele, which did not conform to Hardy Weinberg equilibrium law (P01). At the age of 12 weeks, There was no significant difference in production performance among each genotype (P 0.05). At the age of 18 weeks, the intermuscular fat width of BB genotype was significantly higher than that of AA genotype (P 0.05), and the abdominal fat weight of), BB and AB genotype was significantly higher than that of AA genotype (P 0.01), and the abdominal fat percentage was significantly higher than that of AA genotype (P 0.05). At the age of 24 weeks, the body weight, intermuscular fat width and abdominal fat weight of BB genotype were significantly higher than those of AA genotype (P 0.05). It is speculated that A-FABP gene can be used as one of the candidate markers related to fat traits of Baicheng oil chicken rooster.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31260550)
【分類號(hào)】：S831

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