【摘要】：2014年-2015年陜西楊凌地區(qū)部分商品蛋雞場產(chǎn)蛋量下降了10%~40%,剖檢部分雞只發(fā)現(xiàn)肝臟腫大出血。為確診該疫情的病因,從發(fā)病的6個蛋雞場采集了337份血清和268份糞便,利用間接ELISA和套式RT-PCR分別對血清中的禽戊型肝炎病毒(HEV)抗體和糞便中的禽HEV RNA進(jìn)行了實(shí)驗室檢測。ELISA檢測結(jié)果發(fā)現(xiàn),168份血清為禽HEV抗體陽性,陽性率為49.85%;套式RT-PCR檢測結(jié)果發(fā)現(xiàn),63份糞便為禽HEV RNA基因片段陽性,陽性率為23.51%。基因片段的序列同源性分析發(fā)現(xiàn),楊凌地區(qū)的禽HEV分離株與國內(nèi)的同源性為93.0%~99.2%;進(jìn)化樹分析表明,其與國內(nèi)其他地區(qū)的分離株在同一進(jìn)化分支上,同屬禽HEV基因3型。
[Abstract]:From 2014 to 2015, the egg production of some commercial laying hens in Yang Ling area of Shaanxi Province decreased by 10% to 40%. Some chickens were found to have massive liver bleeding. In order to confirm the cause of the epidemic, 337 serum and 268 faeces were collected from 6 laying hens. Indirect ELISA and nest RT-PCR were used to detect avian hepatitis E virus (HEV) antibody in serum and avian HEV RNA in feces, respectively. the results of Elisa showed that 168 serum samples were positive for avian HEV antibody, the positive rate was 49.85%. The results of nest RT-PCR showed that 63 feces were positive for avian HEV RNA gene fragment, the positive rate was 23.51%. The sequence homology analysis of the gene fragment showed that the homology between the avian HEV isolate in Yang Ling area and that in China was 93.0% 鈮，

本文編號：2479705