【摘要】：蛋雞脂肪肝出血綜合征(FLHS)是蛋雞體內(nèi)脂肪代謝紊亂引起的以肝臟發(fā)生脂肪變性、出血為特征的一種營(yíng)養(yǎng)代謝病,是高產(chǎn)蛋雞常見(jiàn)多發(fā)病。高能低蛋白日糧是我國(guó)目前生產(chǎn)上誘發(fā)蛋雞脂肪肝出血綜合征的主要原因之一。近年來(lái)研究發(fā)現(xiàn)一磷酸腺苷(AMP)激活的蛋白激酶(AMPK)在能量代謝中起著重要作用(特別是在脂質(zhì)代謝中)。本試驗(yàn)旨在研究AMPK信號(hào)通路與高能低蛋白日糧致蛋雞脂肪肝出血綜合征之間的關(guān)系,選用200羽海蘭褐蛋雞隨機(jī)分成兩組,對(duì)照組和試驗(yàn)組,每組5個(gè)重復(fù),每個(gè)重復(fù)20羽,對(duì)照組飼喂基礎(chǔ)日糧代謝能(2678.99 kcal/kg,粗蛋白15.86%),試驗(yàn)組飼喂高能低蛋白日糧(代謝能3100.00 kcal/kg,粗蛋白12.00%),試驗(yàn)期為100d,分別于試驗(yàn)的第20d、40d、60d、80d和100d每組隨機(jī)選取20羽蛋雞進(jìn)行采樣,采集血液、肝臟和下丘腦組織,制作肝臟組織的病理切片,檢測(cè)血清和肝臟組織中的TG、TC、LDL、HDL和NEFA的含量,檢測(cè)肝臟和下丘腦中AMPK、ACC、LKB1、HMGR、FAS、GPAT等相關(guān)因子的mRNA的表達(dá)量。結(jié)果如下:1.試驗(yàn)組大量肝細(xì)胞腫脹,細(xì)胞膜模糊;胞漿內(nèi)可見(jiàn)數(shù)量不等、大小不一的脂肪空泡;細(xì)胞核被推向一側(cè)或消失。2.與對(duì)照組比較,試驗(yàn)組血清和肝臟組織勻漿中TG、TC、LDL和NEFA的含量顯著上升,HDL的含量顯著下降(P0.05)。3.與對(duì)照組比較,試驗(yàn)組肝臟和下丘腦中的ACC、HMGR、FAS、GPAT、HNF4α和PPARα的mRNA的表達(dá)量顯著上升(P0.05),AMPKα1、LKB1和CPT1的mRNA的表達(dá)量顯著下降(P0.05)。結(jié)論:高能低蛋白日糧誘導(dǎo)的蛋雞脂肪肝出血綜合征能夠引起肝臟的脂肪病變,引起肝臟和下丘腦中AMPK的mRNA的表達(dá)量下降,造成脂肪酸和膽固醇的合成增多,而脂肪酸和膽固醇的分解減少,進(jìn)而加劇了蛋雞肝臟中的膽固醇和脂質(zhì)沉積。
[Abstract]:Fatty liver hemorrhage syndrome (FLHS) is a nutritional metabolic disease characterized by fatty degeneration and hemorrhage of liver caused by fat metabolism disorder in laying hens. It is a common and frequent disease in high-yielding laying hens. High energy and low egg white diet is one of the main causes of fatty liver bleeding syndrome in laying hens. In recent years, it has been found that adenosine monophosphate (AMP)-activated protein kinase (AMPK) plays an important role in energy metabolism (especially in lipid metabolism). The purpose of this study was to study the relationship between AMPK signaling pathway and fatty liver hemorrhage syndrome in laying hens induced by high energy and low egg diet. The two groups were randomly divided into two groups: control group and experimental group. The control group was fed with basal diet (2678.99 kcal/kg, crude protein 15.86%), the experimental group was fed with high energy low egg white diet (metabolic energy 3100.00 kcal/kg, crude protein 12.00%), the experimental period was 100 days, respectively, on the 20th day of the experiment, the experimental group was fed with high energy low egg white diet (3100.00 kcal/kg, crude protein 12.00%). At 40 days, 60 days, 80 days and 100 days, 20 layers of hens in each group were randomly selected to sample the blood, liver and hypothalamus tissue, make pathological sections of liver tissue, and detect the contents of TG,TC,LDL,HDL and NEFA in serum and liver tissue. The expression of mRNA of AMPK,ACC,LKB1,HMGR,FAS,GPAT and other related factors in liver and hypothalamus were detected. The results are as follows: 1. In the experimental group, a large number of hepatocytes were swollen and the cell membrane was blurred; fat vacuoles were seen in the cytoplasm in varying numbers and sizes; the nucleus was pushed to one side or disappeared. Compared with the control group, the contents of TG,TC,LDL and NEFA in serum and liver homogenate of the experimental group increased significantly, while the content of HDL decreased significantly (P0.05). Compared with the control group, the mRNA expression of ACC,HMGR,FAS,GPAT, HNF4偽 and PPAR 偽 in the liver and hypothalamus of the experimental group increased significantly (P0.05), while the mRNA expression of AMPK偽 1, LKB1 and CPT1 decreased significantly (P0.05). Conclusion: fatty liver hemorrhage syndrome induced by high-energy and low-egg diet can cause fatty liver fat lesion, decrease the expression of AMPK mRNA in liver and hypothalamus, and increase the synthesis of fatty acids and cholesterol. The degradation of fatty acids and cholesterol increases cholesterol and lipid deposition in the liver of laying hens.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.31

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