發(fā)布時(shí)間：2019-04-26 02:09

【摘要】：為研究FoxO4、PRKAA1基因在從江香豬不同組織中的表達(dá)情況,本實(shí)驗(yàn)采用實(shí)時(shí)熒光定量PCR技術(shù),檢測FoxO4、PRKAA1基因的mRNA表達(dá)量,分析FoxO4、PRKAA1在貴州從江香豬不同組織的差異表達(dá)。結(jié)果顯示,FoxO4基因mRNA在從江香豬所檢測的9個(gè)組織樣中均有表達(dá),其中在脂肪組織中表達(dá)量最高,且差異極顯著(p0.01),在背最長肌組織中表達(dá)量最低;PRKAA1基因mRNA在從江香豬所檢測的9個(gè)組織樣中也均有表達(dá),其中在肺組織中表達(dá)量最高,且差異極顯著(p0.01),在脂肪組織中表達(dá)量次之,在背最長肌組織中表達(dá)量最低。FoxO4、PRKAA1基因可能在肌內(nèi)脂肪(IMF)沉積中發(fā)揮重要作用,為進(jìn)一步研究FoxO4、PRKAA1的功能奠定基礎(chǔ)。
[Abstract]:In order to study the expression of FoxO4,PRKAA1 gene in different tissues of Congjiang Xiang pig, the mRNA expression of FoxO4,PRKAA1 gene was detected by real-time fluorescence quantitative PCR, and the differential expression of FoxO4,PRKAA1 in different tissues of Congjiang Xiang pig in Guizhou province was analyzed. The results showed that mRNA of FoxO4 gene was expressed in 9 tissue samples detected from Jiangxiang pig, among which the expression level in adipose tissue was the highest, and the difference was very significant (p0.01), and the expression level in dorsum longissimus muscle was the lowest. MRNA of PRKAA1 gene was also expressed in 9 tissue samples detected from Jiangxiang pig, among which the expression level in lung tissue was the highest, and the difference was very significant (p0.01), and the expression quantity in adipose tissue was the second, and the expression in adipose tissue was higher than that in adipose tissue (p0.01). FoxO4, PRKAA1 gene may play an important role in the deposition of (IMF) in intramuscular fat, which lays a foundation for further study of the function of FoxO4,PRKAA1.
【作者單位】： 高原山地動(dòng)物遺傳育種與繁殖省部共建教育部重點(diǎn)實(shí)驗(yàn)室;貴州大學(xué)動(dòng)物科學(xué)學(xué)院;貴州省動(dòng)物遺傳育種與繁殖重點(diǎn)實(shí)驗(yàn)室;貴州大學(xué)生命科學(xué)學(xué)院;
【基金】：國家科技支撐計(jì)劃“貴州香豬特色性狀評估及特色基因挖掘與功能研究”[2015BAD03B02-3] 貴州畜禽種質(zhì)資源保存創(chuàng)新與利用[黔科合重大專項(xiàng)字(2013)6008] 貴州大學(xué)研究生創(chuàng)新基金[研農(nóng)2016021]項(xiàng)目基金共同資助
【分類號(hào)】：S828
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本文編號(hào)：2465663