奶山羊FABP3和FABP4基因的多態(tài)性與乳脂肪酸成分的關(guān)聯(lián)分析
發(fā)布時(shí)間：2019-04-24 07:24
【摘要】：脂肪酸結(jié)合蛋白(Fatty acid binding protein,FABP)是與胞內(nèi)脂質(zhì)相結(jié)合的一類蛋白,其對長鏈脂肪酸具有高度的親和力。長鏈脂肪酸從血液進(jìn)入細(xì)胞后,需與FABP結(jié)合,運(yùn)輸?shù)教囟ǖ陌邢蚣?xì)胞器。因此在動(dòng)物體內(nèi),FABP對脂肪酸的攝取、胞內(nèi)轉(zhuǎn)運(yùn)、脂化、氧化以及合成均有重要作用。在奶山羊的乳腺組織中,FABP3和FABP4基因的表達(dá)量豐富,故FABP3和FABP4基因遺傳信息的變化,將直接或間接地調(diào)控羊奶成分與脂肪酸的組成。本試驗(yàn)以西農(nóng)薩能奶山羊?yàn)檠芯繉ο?分析FABP3和FABP4基因的遺傳多態(tài)性,采集不同月份泌乳羊奶樣(泌乳30 d,60 d,90 d,120 d)并分析其主要乳成分和脂肪酸組成,篩選出顯著性影響奶山羊泌乳性狀的SNP。主要研究結(jié)果如下:(1)采用DNA池測序和PCR-RFLP等方法研究了FABP3和FABP4基因的遺傳變異,檢測到4個(gè)新的多態(tài)位點(diǎn),分別位于FABP3基因第一外顯子的SNP G-986-A,FABP3基因第三內(nèi)含子的SNP C-3064-A,FABP4基因第二外顯子的SNP G-3632-A,FABP4基因第二內(nèi)含子的SNP A-3716-G。其中SNP G-986-A為錯(cuò)義突變(Cys20Tyr),SNP G-3632-A為同義突變(Arg60Arg)。(2)通過對FABP3和FABP4基因多態(tài)性的分析,發(fā)現(xiàn)4個(gè)多態(tài)位點(diǎn)的基因(型)頻率,其多態(tài)信息含量均在30%以上,并符合哈代-溫伯格平衡定律。其中,FABP4基因的SNP G-3632-A和SNP A-3716-G高度連鎖,FABP4基因的單倍型-GA-出現(xiàn)的頻率最高,達(dá)59.0%。(3)羊奶中測得37種脂肪酸,其中油酸含量最高,羊奶中飽和脂肪酸與不飽和脂肪酸含量的比例為2:1。隨著泌乳時(shí)間的延長,羊奶中飽和脂肪酸的含量增加,然而不飽和脂肪酸的含量降低。(4)對FABP3和FABP4基因的多態(tài)位點(diǎn)與羊奶成分進(jìn)行關(guān)聯(lián)分析,發(fā)現(xiàn)FABP3基因的SNP G-986-A位點(diǎn)與奶山羊的乳脂、總脂固形物和長鏈脂肪酸的含量以及脂肪酸的不飽和度顯著相關(guān);FABP3基因的SNP C-3064-A位點(diǎn)與奶山羊的長鏈脂肪酸含量以及脂肪酸的不飽和度顯著相關(guān);FABP4基因的SNP G-3632-A與奶山羊的乳脂、乳蛋白、總脂固形物、非脂固形物和長鏈脂肪酸含量以及脂肪酸的不飽和度顯著相關(guān);FABP4基因的SNP A-3716-G與奶山羊的長鏈脂肪酸含量以及脂肪酸的不飽和度顯著相關(guān)。
[Abstract]:Fatty acid binding protein (Fatty acid binding protein,FABP (fatty acid binding protein) is a kind of protein that binds to intracellular lipid and has high affinity to long chain fatty acid. After entering cells from blood, long chain fatty acids bind to FABP and transport to specific targeted organelles. Therefore, FABP plays an important role in fatty acid uptake, intracellular transport, lipid formation, oxidation and synthesis in animals. The expression levels of FABP3 and FABP4 genes are abundant in breast tissues of dairy goats. Therefore, the changes of genetic information of FABP3 and FABP4 genes will directly or indirectly regulate the composition of goat milk components and fatty acids. The genetic polymorphisms of FABP3 and FABP4 genes were analyzed in Xinong Saanen dairy goats in this experiment. Milk samples (30 d, 60 d, 90 d, 120 d) of lactating sheep in different months were collected and their main milk composition and fatty acid composition were analyzed. Screening of SNP. with significant effect on lactation of Dairy Goat The main results are as follows: (1) the genetic variation of FABP3 and FABP4 genes was studied by DNA pool sequencing and PCR-RFLP. In the third intron of the FABP3 gene, the SNP Cx3064A, the second exon of the FABP4 gene, and the second intron of the FABP4 gene, the SNP GX3632A, and the second intron of the FABP4 gene, SNP Ax3716G. Among them, SNP GX986A is missense mutation (Cys20Tyr), SNP GX3632A is synonymous mutation (Arg60Arg). (2) by analyzing the polymorphism of FABP3 and FABP4 genes, the gene frequencies of four polymorphic loci were found, and the gene frequencies of the four polymorphic loci were detected by the analysis of the polymorphism of the FABP3 and FABP4 genes. The polymorphic information content is more than 30% and accords with Hardy-Weinberg equilibrium law. The haplotype-GA- of FABP4 gene had the highest frequency of 59.0%. (3) 37 kinds of fatty acids were detected in goat milk, among which oleic acid content was the highest, and the frequency of haplotype-GA- of FABP4 gene was the highest in goat milk, the highest in which oleic acid content was found in sheep milk, and the frequency of haplotype-GA- in FABP4 gene was the highest (59.0%). The ratio of saturated fatty acid to unsaturated fatty acid in goat milk is 2. With the prolongation of lactation time, the content of saturated fatty acids in goat milk increased, but the content of unsaturated fatty acids decreased. (4) the polymorphism sites of FABP3 and FABP4 genes were associated with the composition of goat milk. It was found that there was a significant correlation between the SNP G / 986 A site of FABP3 gene and milk fat, total fat solids, long chain fatty acids and unsaturation of fatty acids in dairy goats. There was a significant correlation between the SNP C _ (3 064) A site of FABP3 gene and the content of long chain fatty acids and the unsaturation of fatty acids in dairy goats. The SNP of FABP4 gene was significantly correlated with milk fat, milk protein, total fat solids, non-fat solids, long-chain fatty acids and unsaturation of fatty acids in dairy goats. The SNP of FABP4 gene was significantly correlated with the content of long chain fatty acids and the unsaturation of fatty acids in dairy goats.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S827
本文編號：2464246
[Abstract]:Fatty acid binding protein (Fatty acid binding protein,FABP (fatty acid binding protein) is a kind of protein that binds to intracellular lipid and has high affinity to long chain fatty acid. After entering cells from blood, long chain fatty acids bind to FABP and transport to specific targeted organelles. Therefore, FABP plays an important role in fatty acid uptake, intracellular transport, lipid formation, oxidation and synthesis in animals. The expression levels of FABP3 and FABP4 genes are abundant in breast tissues of dairy goats. Therefore, the changes of genetic information of FABP3 and FABP4 genes will directly or indirectly regulate the composition of goat milk components and fatty acids. The genetic polymorphisms of FABP3 and FABP4 genes were analyzed in Xinong Saanen dairy goats in this experiment. Milk samples (30 d, 60 d, 90 d, 120 d) of lactating sheep in different months were collected and their main milk composition and fatty acid composition were analyzed. Screening of SNP. with significant effect on lactation of Dairy Goat The main results are as follows: (1) the genetic variation of FABP3 and FABP4 genes was studied by DNA pool sequencing and PCR-RFLP. In the third intron of the FABP3 gene, the SNP Cx3064A, the second exon of the FABP4 gene, and the second intron of the FABP4 gene, the SNP GX3632A, and the second intron of the FABP4 gene, SNP Ax3716G. Among them, SNP GX986A is missense mutation (Cys20Tyr), SNP GX3632A is synonymous mutation (Arg60Arg). (2) by analyzing the polymorphism of FABP3 and FABP4 genes, the gene frequencies of four polymorphic loci were found, and the gene frequencies of the four polymorphic loci were detected by the analysis of the polymorphism of the FABP3 and FABP4 genes. The polymorphic information content is more than 30% and accords with Hardy-Weinberg equilibrium law. The haplotype-GA- of FABP4 gene had the highest frequency of 59.0%. (3) 37 kinds of fatty acids were detected in goat milk, among which oleic acid content was the highest, and the frequency of haplotype-GA- of FABP4 gene was the highest in goat milk, the highest in which oleic acid content was found in sheep milk, and the frequency of haplotype-GA- in FABP4 gene was the highest (59.0%). The ratio of saturated fatty acid to unsaturated fatty acid in goat milk is 2. With the prolongation of lactation time, the content of saturated fatty acids in goat milk increased, but the content of unsaturated fatty acids decreased. (4) the polymorphism sites of FABP3 and FABP4 genes were associated with the composition of goat milk. It was found that there was a significant correlation between the SNP G / 986 A site of FABP3 gene and milk fat, total fat solids, long chain fatty acids and unsaturation of fatty acids in dairy goats. There was a significant correlation between the SNP C _ (3 064) A site of FABP3 gene and the content of long chain fatty acids and the unsaturation of fatty acids in dairy goats. The SNP of FABP4 gene was significantly correlated with milk fat, milk protein, total fat solids, non-fat solids, long-chain fatty acids and unsaturation of fatty acids in dairy goats. The SNP of FABP4 gene was significantly correlated with the content of long chain fatty acids and the unsaturation of fatty acids in dairy goats.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S827
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 許會(huì)芬;羅軍;朱越;朱江江;李君;杜鵑;;日糧中不同能量和蛋白水平對西農(nóng)薩能羊泌乳量、乳成分及血漿生化指標(biāo)的影響[J];黑龍江畜牧獸醫(yī);2013年01期
，本文編號：2464246
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