【摘要】：為揭示南方黃牛肉質(zhì)調(diào)控的分子機(jī)制并挖掘與肌肉嫩度相關(guān)的功能基因,本研究以1周歲左右的云嶺牛、文山牛和中國(guó)西門塔爾牛為試驗(yàn)對(duì)象,屠宰后測(cè)定背最長(zhǎng)肌不同排酸時(shí)間(0、1、2、3、5、7 d)下剪切力變化,揭示不同黃牛品種肌肉嫩度的差異,利用RNA-Seq技術(shù)對(duì)背最長(zhǎng)肌進(jìn)行比較轉(zhuǎn)錄組學(xué)分析,并結(jié)合生物信息學(xué)篩選與肌肉嫩度調(diào)控相關(guān)的信號(hào)通路及差異表達(dá)基因,最后利用q PCR進(jìn)行驗(yàn)證。結(jié)果表明:在宰后成熟過(guò)程中,黃牛肌肉嫩度表現(xiàn)為明顯的下降趨勢(shì),且在同一個(gè)成熟時(shí)間點(diǎn)下,剪切力值表現(xiàn)為西門塔爾牛文山牛云嶺牛,表明云嶺牛嫩度較好,而西門塔爾牛嫩度相對(duì)較差;云嶺牛與西門塔爾牛背最長(zhǎng)肌2組文庫(kù)經(jīng)測(cè)序后,篩選出3 198個(gè)差異表達(dá)基因,其中云嶺牛上調(diào)基因1 750個(gè),KEGG功能注釋發(fā)現(xiàn)2個(gè)與肉質(zhì)調(diào)控相關(guān)的重要信號(hào)通路:脂肪細(xì)胞因子信號(hào)通路和內(nèi)質(zhì)網(wǎng)蛋白加工通路,結(jié)合q PCR驗(yàn)證、基因表達(dá)水平與剪切力值相關(guān)性分析,最終篩選出Leptin和CAPN12個(gè)可能肌肉嫩度相關(guān)的功能基因。本研究篩選與鑒定出南方黃牛肌肉嫩度相關(guān)信號(hào)通路及其關(guān)鍵基因,為今后南方黃牛肉質(zhì)遺傳改良奠定理論基礎(chǔ)。
[Abstract]:In order to reveal the molecular mechanism of meat quality regulation in southern yellow cattle and excavate functional genes related to muscle tenderness, Yunling cattle, Wenshan cattle and Chinese Simmental cattle aged about one year were selected as experimental subjects. In order to reveal the difference of muscle tenderness of different yellow cattle breeds, the muscle tenderness of the longest dorsum muscle was analyzed by RNA-Seq technique, and the changes of shear stress at different times (0, 1, 2, 3, 5, 7 days) were measured after slaughter, and the muscle tenderness of the longissimus dorsi muscle was compared with that of the longissimus dorsi muscle by means of RT-PCR. Combined with bioinformatics, signal pathways and differentially expressed genes related to muscle tenderness regulation were screened and verified by Q-PCR. The results showed that the muscle tenderness of yellow cattle decreased obviously during postmortem ripening, and at the same mature time point, the shear force was that of Simmental Niuwenshan Niu Yunling cattle, which indicated that Yunling cattle had better tenderness. The tenderness of Simmental cattle is relatively poor; After sequencing, 3 198 differentially expressed genes were screened between Yunling cattle and Simmental cattle. Among them, 1,750 genes were up-regulated in Yunling cattle. KEGG functional annotation found two important signaling pathways related to meat quality regulation: adipocytokine signaling pathway and endoplasmic reticulum protein processing pathway. Combined with Q-PCR verification, the correlation between gene expression level and shear force was analyzed. Finally, the functional genes of Leptin and CAPN12 which may be related to muscle tenderness were screened out. In this study, the signal pathway and key genes related to muscle tenderness of southern yellow cattle were screened and identified, which laid a theoretical foundation for genetic improvement of meat quality of southern yellow cattle in the future.
【作者單位】： 揚(yáng)州大學(xué)旅游烹飪學(xué)院;云南省草地與動(dòng)物研究院;
【基金】：863國(guó)家高技術(shù)研究發(fā)展計(jì)劃項(xiàng)目(2013AA102505-5)
【分類號(hào)】：S823.2

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