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應(yīng)用CNCPS方法和體外產(chǎn)氣法研究豆腐渣飼料的營(yíng)養(yǎng)價(jià)值

發(fā)布時(shí)間:2019-03-28 20:48
【摘要】:為揭示豆腐渣對(duì)反芻動(dòng)物的飼用價(jià)值,本試驗(yàn)采用康奈爾凈碳水化合物—蛋白質(zhì)體系(CNCPS)和體外產(chǎn)氣法對(duì)豆腐渣營(yíng)養(yǎng)價(jià)值進(jìn)行評(píng)定。測(cè)定豆腐渣的CNCPS營(yíng)養(yǎng)成分與體外發(fā)酵參數(shù),應(yīng)用CNCPS公式計(jì)算碳水化合物(CHO)和粗蛋白質(zhì)(CP)中各組分含量。結(jié)果顯示,豆腐渣的CP含量為14%~25%,粗脂肪(EE)含量為1.9%~2.9%,代謝能(ME)為10~12 MJ/kg DM;易在瘤胃中降解的蛋白質(zhì)(PA+PB1)和在瘤胃中緩慢降解的粗蛋白質(zhì)(PB2+PB3)的含量相當(dāng),不可降解真蛋白質(zhì)(PC)含量較少;潛在可利用碳水化合物(CA+CB1+CB2)較高;體外發(fā)酵產(chǎn)氣量均達(dá)到70mL,氨態(tài)氮(NH3-N)濃度在8~23mg/dL,總揮發(fā)性脂肪酸(TVFA)在46~70mmol/L。而不同來(lái)源的豆腐渣營(yíng)養(yǎng)成分和發(fā)酵情況存在顯著差異,且CNCPS碳水化合物、蛋白質(zhì)組分的優(yōu)劣不同。凌源豆腐渣的最大產(chǎn)氣量(B)最高,極顯著高于其他3種蘋果渣(P0.01),而各豆腐渣的產(chǎn)氣速度(C)無(wú)顯著差異(P0.05)。遼源豆腐渣氨態(tài)氮(NH3-N)含量最高,極顯著高于其他飼料(P0.01)。不同地方豆腐渣瘤胃液揮發(fā)性脂肪酸比例差異極顯著(P0.01)。不同來(lái)源的豆腐渣的總可消化養(yǎng)分(TDN)及其他能值差異較大,以新民豆腐渣的能值最低,極顯著低于其他蘋果渣(P0.01)。綜上可知,作為反芻動(dòng)物非糧飼料,豆腐渣為反芻動(dòng)物提供的可利用能值高,利于瘤胃微生物蛋白質(zhì)合成。
[Abstract]:In order to reveal the feeding value of bean curd residue for ruminants, Cornell's net carbohydrate-protein system (CNCPS) and in vitro gas production method were used to evaluate the nutritional value of soybean curd residue. The nutrient composition of CNCPS and fermentation parameters in vitro were determined. The contents of carbohydrate (CHO) and crude protein (CP) were calculated by CNCPS formula. The results showed that the content of CP in dregs of bean curd was 14% and 25%, the content of (EE) in crude fat was 1.9% and 2.9%, and the metabolizable energy (ME) was 10? 12 MJ/kg DM;. The content of protein (PA PB1) easily degraded in rumen was similar to that of crude protein (PB2 PB3) which degraded slowly in rumen, and the content of (PC) in non-degradable real protein was lower, and the potential available carbohydrate (CA CB1 CB2) was higher. The in vitro gas production was 70 mL, the concentration of ammonia nitrogen (NH3-N) was 8-23 mg / L, and the total volatile fatty acid (TVFA) was 46-70 mmol 路L ~ (- 1) 路h ~ (- 1). There were significant differences in nutrient composition and fermentation status of soybean curd residue from different sources, and the advantages and disadvantages of CNCPS carbohydrate and protein components were different. The maximum gas production rate (B) of Lingyuan bean curd dregs was the highest, which was significantly higher than that of the other three kinds of apple dregs (P0.01), but there was no significant difference in the gas production rate (C) of each bean curd dregs (P0.05). The content of ammonia nitrogen (NH3-N) in Liaoyuan bean curd residue was the highest, which was significantly higher than that of other diets (P0.01). There was significant difference in the proportion of volatile fatty acids in rumen juice of bean curd residue between different places (P0.01). The total digestible nutrients (TDN) and other energy values of soybean curd residue from different sources were significantly different. The energy value of Xinmin dregs was the lowest, which was significantly lower than that of other apple dregs (P0.01). In conclusion, as a non-food feed for ruminants, bean curd residue provides high available energy for ruminants, which is beneficial to rumen microbial protein synthesis.
【作者單位】: 中國(guó)農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院動(dòng)物營(yíng)養(yǎng)學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】:國(guó)家自然科學(xué)基金面上項(xiàng)目(31372335) 國(guó)家肉牛牦牛產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-38) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)經(jīng)費(fèi)項(xiàng)目(201503134)
【分類號(hào)】:S816
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本文編號(hào):2449217

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