種番鴨源巴泰病毒的分離及鑒定
發(fā)布時(shí)間：2019-03-27 11:59
【摘要】：為確定引起種番鴨產(chǎn)蛋下降的病原,本研究應(yīng)用透射電鏡負(fù)染及理化特性測(cè)定等方法對(duì)1株分離自表現(xiàn)產(chǎn)蛋下降的283日齡種番鴨卵巢的病毒(ZJ1408株)進(jìn)行研究,結(jié)合RT-PCR檢測(cè)及序列分析結(jié)果,確定所分離病毒為布尼亞病毒屬的巴泰病毒(BATV)。該病毒可在鴨胚成纖維細(xì)胞和Vero增殖并引起細(xì)胞病變。對(duì)病毒核蛋白部分序列測(cè)定與分析發(fā)現(xiàn),該病毒與已發(fā)布的鴨源分離株ZJ14、牛源分離株XQ-B及NM/12核苷酸序列同源性在99.5%以上,與近年來(lái)報(bào)道的基因重配變異株Ngari的同源性為92%左右;遺傳進(jìn)化分析表明,該病毒株與早期蚊蟲源BATV分離株MM2222、G-20217及804986等同處于亞-非基因型進(jìn)化分支。以上結(jié)果表明我國(guó)飼養(yǎng)的鴨群存在BATV感染,這提示應(yīng)充分重視該類人畜共患病病原在家禽中流行的監(jiān)測(cè)。
[Abstract]:In order to determine the pathogen causing egg loss of Muscovy duck, transmission electron microscopy (TEM) and physicochemical analysis were used to study the virus (ZJ1408 strain) isolated from the ovary of 283-day-old Muscovy duck with decreased egg production. Combined with the results of RT-PCR detection and sequence analysis, the isolated virus was identified as Bunia virus (BATV)., which belongs to the genus Bunia virus. The virus can proliferate and cause cytopathic effect in duck embryo fibroblasts and Vero. Partial sequencing and analysis of nucleocapsid protein of the virus showed that the nucleotide sequence homology of the virus with the published ZJ14, bovine isolate XQ-B and NM/12 was more than 99.5%. The homology was about 92% with the gene reassorted mutants Ngari reported in recent years. Genetic evolution analysis showed that the virus strain was in sub-nongenotypic evolutionary branch with early mosquito-derived BATV isolates MM2222,G-20217 and 804986. The above results suggest that BATV infection exists in duck herds in China, which suggests that the surveillance of zoonosis in poultry should be paid more attention to.
【作者單位】： 福建省農(nóng)科院畜牧獸醫(yī)研究所福建省畜禽疫病防治工程技術(shù)研究中心/福建省禽病防治重點(diǎn)實(shí)驗(yàn)室;解放軍南京軍區(qū)福州總醫(yī)院病理科;
【基金】：福建省屬公益類科研院所基本科研項(xiàng)目(2017R1023-16)
【分類號(hào)】：S852.65
本文編號(hào)：2448154
[Abstract]:In order to determine the pathogen causing egg loss of Muscovy duck, transmission electron microscopy (TEM) and physicochemical analysis were used to study the virus (ZJ1408 strain) isolated from the ovary of 283-day-old Muscovy duck with decreased egg production. Combined with the results of RT-PCR detection and sequence analysis, the isolated virus was identified as Bunia virus (BATV)., which belongs to the genus Bunia virus. The virus can proliferate and cause cytopathic effect in duck embryo fibroblasts and Vero. Partial sequencing and analysis of nucleocapsid protein of the virus showed that the nucleotide sequence homology of the virus with the published ZJ14, bovine isolate XQ-B and NM/12 was more than 99.5%. The homology was about 92% with the gene reassorted mutants Ngari reported in recent years. Genetic evolution analysis showed that the virus strain was in sub-nongenotypic evolutionary branch with early mosquito-derived BATV isolates MM2222,G-20217 and 804986. The above results suggest that BATV infection exists in duck herds in China, which suggests that the surveillance of zoonosis in poultry should be paid more attention to.
【作者單位】： 福建省農(nóng)科院畜牧獸醫(yī)研究所福建省畜禽疫病防治工程技術(shù)研究中心/福建省禽病防治重點(diǎn)實(shí)驗(yàn)室;解放軍南京軍區(qū)福州總醫(yī)院病理科;
【基金】：福建省屬公益類科研院所基本科研項(xiàng)目(2017R1023-16)
【分類號(hào)】：S852.65
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