【摘要】：內(nèi)蒙古絨山羊是我國絨山羊珍貴品種之一,其中絨纖維經(jīng)濟價值較高,素有“纖維寶石”之稱。通過前人研究,發(fā)現(xiàn)褪黑激素能夠促進絨毛生長,但其信號通路及調控機理尚不清楚。因此,本研究以內(nèi)蒙古絨山羊為研究對象,在耳后皮下埋植褪黑激素,對絨毛生長特性與毛品質進行檢測,并利用Western Blot和免疫熒光技術探討褪黑激素對核受體RORa在內(nèi)蒙古絨山羊2、9、12月份皮膚毛囊中蛋白水平的表達及分布的影響。研究得到結果如下：(1)絨毛生長特性與毛品質檢測結果表明：試驗組與對照組的羊絨細度在9月份和12月份差異顯著(P0.05),其他各月份差異均不顯著(P0.05)；試驗組與對照組羊絨長度在9、10、11、1、2月份和3月份差異均顯著(P0.05),8、12和4月份差異均不顯著(P0.05)；結果表明埋植褪黑素有促進絨毛變長的趨勢；試驗組與對照組的產(chǎn)絨量差異不顯著(P0.05),但產(chǎn)絨量有增加的趨勢。結果表明褪黑激素對絨毛的長度影響較大、對絨毛細度和產(chǎn)絨量影響不大,但是產(chǎn)絨量有提高的趨勢。(2) Western Blot結果表明：RORa蛋白在2、9、12月份的對照組和試驗組的皮膚毛囊中均表達,且2月份表達量最高,9月份下降,12月份最低;2月份試驗組RORa蛋白表達量顯著高于對照組(P0.05);在9、12月份試驗組與對照組組RORa蛋白表達量均差異不顯著(P0.05),結果表明褪黑激素上調了2月份RORa蛋白的表達。(3)免疫熒光染色結果表明：對照組中2、9、12月份RORa蛋白只在毛囊外根鞘表達,而試驗組中3個月份RORa蛋白在毛囊外根鞘和毛乳頭中均有表達。本試驗研究埋植和不埋植褪黑激素對絨毛產(chǎn)量與絨毛品質的影響,利用Western Blot技術和免疫熒光檢測技術,分析埋植褪黑激素對絨山羊皮膚毛囊中RORa表達的影響,結果表明褪黑激素促進絨毛生長是與毛囊外根鞘和毛乳頭中的RORa結合,進而參與調節(jié)毛囊周期性生長。
[Abstract]:Inner Mongolia cashmere goat is one of the precious breeds of cashmere goat in China, in which cashmere fiber is of high economic value and is known as "fiber gem". By previous studies, it was found that melatonin can promote villus growth, but its signaling pathway and regulation mechanism are not clear. Therefore, in this study, Inner Mongolia cashmere goats were implanted subcutaneously with melatonin in the back of ear to detect the growth characteristics and hair quality of hair. The effects of melatonin on the expression and distribution of nuclear receptor RORa in the hair follicles of Inner Mongolia Cashmere Goats were studied by Western Blot and immunofluorescence techniques. The results were as follows: (1) the results showed that the cashmere fineness of the experimental group and the control group were significantly different in September and December (P0.05), but there was no significant difference in the other months (P0.05). The cashmere lengths of the experimental group and the control group were significantly different in 9, 10, 11, 1, 2 and 3 months (P0.05), but there was no significant difference in 8,12 and April (P0.05), the results showed that melatonin implantation had a tendency to promote villus lengthening. There was no significant difference between the treatment group and the control group (P0.05), but there was an increasing trend of the cashmere yield. The results showed that melatonin had a great effect on the length of villus, but had little effect on the fineness and yield of villus. (2) Western Blot results showed that RORa protein was 2,9, The expression in the hair follicles of the control group and the experimental group in December was the highest in February, decreased in September, and the lowest in December. In February, the expression of RORa protein in experimental group was significantly higher than that in control group (P0.05). There was no significant difference in the expression of RORa protein between the experimental group and the control group in September and December (P0.05), the results showed that melatonin upregulated the expression of RORa protein in February. (3) the immunofluorescence staining results showed that in the control group, the expression of RORa protein was significantly increased. The RORa protein was only expressed in the outer root sheath of the hair follicle in December, while the RORa protein was expressed in the outer root sheath and papilla of the hair follicle in the experiment group for three months. In this study, the effects of melatonin on the yield and quality of cashmere were studied. The effects of melatonin on the expression of RORa in the hair follicles of cashmere goats were analyzed by Western Blot technique and immunofluorescence assay, and the effect of melatonin on the expression of melatonin in the hair follicles of cashmere goats was studied. The results showed that melatonin promoted villous growth by binding to RORa in the outer root sheath and papilla of hair follicles, and then involved in regulating the periodic growth of hair follicles.
【學位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S827

【相似文獻】

相關期刊論文 前10條

1 陳世林,趙書紅,余梅,李孟華,李奎;西藏絨山羊、內(nèi)蒙古絨山羊、遼寧絨山羊隨機擴增多態(tài)DNA研究[J];中國草食動物;2002年S1期

2 松杰;董揚;孟麗云;杜琛;蘇蕊;李金泉;張文廣;;內(nèi)蒙古絨山羊皮膚蛋白質雙向電泳條件優(yōu)化及圖譜建立[J];中國畜牧獸醫(yī);2012年07期

3 朱森陽;楊成和;張永斌;李藏蘭;賈志海;;不同配種周期對內(nèi)蒙古絨山羊繁殖和產(chǎn)絨性能的影響[J];中國畜牧雜志;2005年12期

4 鄭琰;席海燕;周歡敏;;5-溴-2-脫氧尿嘧啶核苷標記內(nèi)蒙古絨山羊皮膚組織的免疫組織化學檢測[J];中國畜牧獸醫(yī);2009年04期

5 鄭琰;席海燕;周歡敏;;5-溴-2-脫氧尿嘧啶核苷標記內(nèi)蒙古絨山羊皮膚組織的免疫組織化學檢測[J];飼料博覽;2009年06期

6 鄭竹清;石玲;王夢一;吳敬;劉志紅;張燕軍;蘇蕊;趙艷紅;;褪黑激素受體1a基因在內(nèi)蒙古絨山羊垂體中的表達[J];中國畜牧獸醫(yī);2012年04期

7 趙艷紅;王夢一;蘇蕊;劉志紅;武瑞兵;張燕軍;李金泉;包躍先;;內(nèi)蒙古絨山羊4個群體遺傳多樣性分析[J];湖北農(nóng)業(yè)科學;2012年08期

8 勿都巴拉;阿如汗;吳江鴻;張燕軍;張文廣;李金泉;;內(nèi)蒙古絨山羊角蛋白5基因啟動子克隆及序列分析[J];中國畜牧獸醫(yī);2012年06期

9 李娜;孔德清;張若竹;郁楓;;內(nèi)蒙古絨山羊毛囊形態(tài)發(fā)生規(guī)律[J];西北農(nóng)業(yè)學報;2013年03期

10 稅世榮;內(nèi)蒙古絨山羊分布及其絨量絨質與生態(tài)條件的關系[J];內(nèi)蒙古畜牧科學;1994年02期

相關會議論文 前8條

1 郭俊清;孫海洲;石巖;凌樹禮;張海鷹;包賽娜;;亮氨酸對內(nèi)蒙古絨山羊免疫機能和骨骼肌蛋白合成的影響研究[A];第六次全國飼料營養(yǎng)學術研討會論文集[C];2010年

2 楊成和;朱森陽;孔祥浩;王潤蓮;賈志海;;不同營養(yǎng)水平對內(nèi)蒙古絨山羊生產(chǎn)性能的影響[A];2006中國羊業(yè)進展——第三屆中國羊業(yè)發(fā)展大會論文集[C];2006年

3 周佳萍;朱森陽;賈志海;朱曉萍;何云梅;;營養(yǎng)調控對內(nèi)蒙古絨山羊繁殖和產(chǎn)絨性能的影響[A];2010中國羊業(yè)進展[C];2010年

4 趙艷紅;王國華;玉榮;杜晨光;張文廣;張燕軍;李金泉;;內(nèi)蒙古絨山羊不同月份皮膚組織RORα基因的表達[A];中國動物遺傳育種研究進展——第十五次全國動物遺傳育種學術討論會論文集[C];2009年

5 趙艷紅;玉榮;劉志紅;張燕軍;李金泉;;MTNR1a基因在內(nèi)蒙古絨山羊卵巢中的表達[A];中國畜牧獸醫(yī)學會養(yǎng)羊學分會全國養(yǎng)羊生產(chǎn)與學術研討會議論文集[C];2010年

6 劉海英;岳春旺;朱曉萍;賈志海;;KAP8.1基因多態(tài)對內(nèi)蒙古絨山羊絨毛性狀的影響[A];中國畜牧獸醫(yī)學會養(yǎng)羊學分會全國養(yǎng)羊生產(chǎn)與學術研討會議論文集[C];2010年

7 趙艷紅;劉志紅;杜晨光;張燕軍;蘇蕊;李金泉;;MTNR1a基因在內(nèi)蒙古絨山羊垂體中的表達[A];第十二次全國畜禽遺傳標記研討會論文集[C];2010年

8 張燕軍;李金泉;李榮;尹俊;高愛琴;張文廣;趙艷紅;蘇蕊;王瑞軍;;內(nèi)蒙古絨山羊BMPR-IB基因部分cDNA序列克隆與在毛囊中的表達[A];中國動物遺傳育種研究進展——第十五次全國動物遺傳育種學術討論會論文集[C];2009年

相關重要報紙文章 前3條

1 王欲鳴邋楊一苗;拯救內(nèi)蒙古絨山羊刻不容緩[N];中國畜牧獸醫(yī)報;2008年

2 本報記者 王欲鳴邋楊一苗;內(nèi)蒙古絨山羊“改良”變“改糟”[N];經(jīng)濟參考報;2008年

3 丁銘;內(nèi)蒙古結構調整對羊絨市場影響不大[N];中國鄉(xiāng)鎮(zhèn)企業(yè)報;2000年

相關博士學位論文 前9條

1 趙s，

本文編號：2440424