【摘要】：�；撬�(Tau)具有良好的抗氧化性,為研究Tau與抗生素聯(lián)合使用對豬精液常溫保存效果的影響,以不含抗生素Modena稀釋液為對照基礎(chǔ)液設(shè)計3組試驗,設(shè)Modena稀釋液+慶大霉素(2×10~5 IU)+復(fù)方新諾明(2.5×10~4 IU)為處理1,Modena基礎(chǔ)液上分別添加0.5、1、5和10mmol/L的Tau為處理2,處理1配伍處理2則為處理3,每24h檢測精子活率、頂體完整率、丙二醛(MDA)濃度以及總抗氧化能力(TAOC),分析Tau與抗生素復(fù)合劑對豬精液常溫保存效果的影響。結(jié)果表明,處理3效果最好,處理2次之,處理1最差,各處理間差異顯著,當(dāng)處理3中Tau的添加量為5mmol/L時,豬精液保存5d后,精子活率為64.11%,頂體完整率為72.10%,T-AOC活性和MDA濃度分別為4.76U/mL和1.42nmol/mL。因此,Tau與抗生素復(fù)合劑可以提高豬精液的常溫保存效果,最適添加濃度為5mmol/L。
[Abstract]:Taurine (Tau) has good antioxidant properties. In order to study the effect of combined use of Tau and antibiotics on the preservation of porcine semen at room temperature, three groups of tests were designed with no antimicrobial diluent as control. The Modena diluent gentamicin (2.5 脳 10 ~ 4 IU) was treated with 0.5, 1, 5 and 10mmol/L Tau as treatment 2, and treatment 1 was treated with 3, 3, 10 ~ 4 IU, respectively, with Tau of 5 and 10mmol/L as treatment 2, 2 as treatment 1, and 2.5 脳 10 ~ 4 IU as treatment 1, and Tau as treatment 2, 5 and 10mmol/L as treatment 2, respectively. Sperm viability, acrosome integrity rate, malondialdehyde (MDA) concentration and total antioxidant capacity (TAOC),) were measured every 24 hours. The effects of Tau and antibiotics on pig semen preservation at room temperature were analyzed. The results showed that the effect of treatment 3 was the best, treatment 2 was the second, treatment 1 was the worst, and there was significant difference among the three treatments. When the addition of Tau in treatment 3 was 5mmol/L, the sperm viability was 64.11% after 5 days of preservation of pig semen. The percentage of intact acrosome was 72.10%, and the activity of 4.76U/mL and the concentration of MDA were 1.42 nmol 路L-1 and 1.42 nmol 路L-1 路L-1, respectively. Therefore, the combination of Tau and antibiotics could improve the storage effect of pig semen at room temperature, and the optimum concentration was 5 mmol / L 路L ~ (- 1) 路h ~ (- 1).
【作者單位】： 西北農(nóng)林科技大學(xué)動物科技學(xué)院;
【基金】：國家生豬產(chǎn)業(yè)技術(shù)體系(CARS-36) 楊凌示范區(qū)科技計劃(2014NY-22) 西北農(nóng)林科技大學(xué)試驗示范站(基地)科技成果推廣(TGZX2015-18)~~
【分類號】：S828

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