豬偽狂犬病毒gB、gC和gD蛋白多抗制備及交叉中和抗體效價測定
發(fā)布時間：2019-03-05 11:59
【摘要】：為研究豬偽狂犬病毒(PRV)主要免疫原性基因的抗原變異情況,本研究構(gòu)建了PRV變異株JS-2012和弱毒疫苗株Bartha-K61的g B、g C和g D基因重組真核表達質(zhì)粒。通過基因槍途徑免疫新西蘭大白兔,對制備的兔源多克隆抗體進行交叉中和抗體效價的測定。結(jié)果顯示,JS-2012和Bartha-K61株的g C蛋白和g D蛋白多抗對PRV JS-2012變異株、SC經(jīng)典強毒株和Bartha-K61弱毒疫苗株的交叉中和效價均無顯著差異;而兩個病毒株的g B蛋白多抗對JS-2012和SC株的中和效價較低,分別為1∶29.0~56.7和1∶56.2~137.0,對Bartha-K61株以及其他毒力基因缺失弱毒疫苗株的中和效價較高,分別為1∶75.0~490.0和1∶198.6~986.9,差異顯著,推測該結(jié)果可能與PRV毒力基因缺失有關(guān)。本研究為進一步鑒定PRV流行株的抗原變異奠定基礎(chǔ)。
[Abstract]:In order to study the antigen variation of major immunogenic genes of porcine pseudorabies virus (PRV), the recombinant eukaryotic expression plasmids of GB, GC and GD genes of PRV variant JS-2012 and attenuated vaccine strain Bartha-K61 were constructed. New Zealand white rabbits were immunized by gene gun. The cross neutralizing antibody titer of rabbit-derived polyclonal antibody was determined. The results showed that there was no significant difference in cross neutralization titer between JS-2012 strain and Bartha-K61 strain against PRV JS-2012 variant strain, SC classical virulent strain and Bartha-K61 attenuated vaccine strain. The neutralization titers of g B protein polyclonal antibody against JS-2012 and SC strains were lower than those of Bartha-K61 strain and SC strain, respectively, and the neutralization titers to Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain were higher than those of Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain, respectively, and the neutralization titers of GB protein polyclonal antibody against Bartha-K61 strain and other virulent genes were higher than those of other virulent genes. The results were 1? 75.0? 490.0 and 1? 198.6? 986.9, respectively. The difference was significant, which suggested that the result might be related to the deletion of virulence gene in PRV. This study lays a foundation for further identification of antigen variation of PRV epidemic strains.
【作者單位】： 中國農(nóng)業(yè)科學院上海獸醫(yī)研究所;
【基金】：上海市自然科學基金項目(14ZR1448900) 上海市科技興農(nóng)重點攻關(guān)項目[滬農(nóng)科攻字(2015)第1-7號] 中央級公益性科研院所基本科研業(yè)務(wù)費專項資金項目(2014JB02)
【分類號】：S852.4
本文編號：2434884
[Abstract]:In order to study the antigen variation of major immunogenic genes of porcine pseudorabies virus (PRV), the recombinant eukaryotic expression plasmids of GB, GC and GD genes of PRV variant JS-2012 and attenuated vaccine strain Bartha-K61 were constructed. New Zealand white rabbits were immunized by gene gun. The cross neutralizing antibody titer of rabbit-derived polyclonal antibody was determined. The results showed that there was no significant difference in cross neutralization titer between JS-2012 strain and Bartha-K61 strain against PRV JS-2012 variant strain, SC classical virulent strain and Bartha-K61 attenuated vaccine strain. The neutralization titers of g B protein polyclonal antibody against JS-2012 and SC strains were lower than those of Bartha-K61 strain and SC strain, respectively, and the neutralization titers to Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain were higher than those of Bartha-K61 strain and other virulence gene deletion weak virus vaccine strain, respectively, and the neutralization titers of GB protein polyclonal antibody against Bartha-K61 strain and other virulent genes were higher than those of other virulent genes. The results were 1? 75.0? 490.0 and 1? 198.6? 986.9, respectively. The difference was significant, which suggested that the result might be related to the deletion of virulence gene in PRV. This study lays a foundation for further identification of antigen variation of PRV epidemic strains.
【作者單位】： 中國農(nóng)業(yè)科學院上海獸醫(yī)研究所;
【基金】：上海市自然科學基金項目(14ZR1448900) 上海市科技興農(nóng)重點攻關(guān)項目[滬農(nóng)科攻字(2015)第1-7號] 中央級公益性科研院所基本科研業(yè)務(wù)費專項資金項目(2014JB02)
【分類號】：S852.4
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