【摘要】：以礦化效率為評價標(biāo)準(zhǔn),對豬圓環(huán)病毒2型(PCV2)生物礦化的反應(yīng)溫度、反應(yīng)時間及Ca/P比、抗原初始p H、添加劑用量等條件進(jìn)行優(yōu)化,并通過小鼠實(shí)驗(yàn)檢測礦化病毒的免疫原性。結(jié)果顯示,生物礦化最優(yōu)處理?xiàng)l件為初始p H 6.5,Ca/P 1.67,4℃礦化12 h,Mg2+/Ca2+為1.0。在該反應(yīng)條件下,病毒粒子能夠形成球形結(jié)構(gòu)的鈣磷PCV2粒子,粒徑為200~500 nm。不同批次PCV2抗原礦化率皆在90%以上,且該方法可去除大量雜蛋白。小鼠免疫礦化PCV2病毒顆粒后14~28 d,抗體水平與商品苗對照組、未礦化PCV2病毒組基本相同,28 d后對照組抗體水平下降明顯,而PCV2礦化顆粒組的抗體基本維持在原水平,表明PCV2礦化顆粒具有緩釋抗原的作用。
[Abstract]:The reaction temperature, reaction time and Ca/P ratio of porcine circovirus type 2 (PCV2) biomineralization, the initial antigen pH, the dosage of additives and so on were optimized. The immunogenicity of mineralized virus was detected by mouse experiment. The results showed that the optimal treatment conditions for biomineralization were as follows: initial pH 6.5 Ca / P 1.67 鈩，

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